BRENDA - Enzyme Database
show all sequences of 3.5.1.84

New family of biuret hydrolases involved in s-triazine ring metabolism

Cameron, S.; Durchschein, K.; Richman, J.; Sadowsky, M.; Wackett, L.; ACS Catal. 1, 1075-1082 (2011)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli DH5alpha cells
Rhizobium leguminosarum bv. viciae
General Stability
General Stability
Organism
potassium phosphate buffer yields optimal catalytic rates
Rhizobium leguminosarum bv. viciae
Inhibitors
Inhibitors
Commentary
Organism
Structure
N-ethylmaleimide
more than 90% loss of activity at 0.05 mM; more than 90% loss of activity at 0.5 mM
Rhizobium leguminosarum bv. viciae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.023
-
Biuret
in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
the addition of ZnSO4 or CoSO4 (0.05 mM and 1 mM) to enzyme assays gives only nominal increases in activity. No metal cofactors are detected
Rhizobium leguminosarum bv. viciae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27121
-
x * 27121, calculated from amino acid sequence
Rhizobium leguminosarum bv. viciae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
biuret + H2O
Rhizobium leguminosarum bv. viciae
the enzyme acts uniquely on biuret physiologically
allophanic acid + NH3
-
-
ir
biuret + H2O
Rhizobium leguminosarum bv. viciae 3841
the enzyme acts uniquely on biuret physiologically
allophanic acid + NH3
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhizobium leguminosarum bv. viciae
-
-
-
Rhizobium leguminosarum bv. viciae 3841
-
-
-
Purification (Commentary)
Commentary
Organism
nickel affinity column chromatography
Rhizobium leguminosarum bv. viciae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.02
-
below 0.02 micromol/min/mg, using 1,1-dimethylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 1-carbamimidoylthiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 1-ethylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 1-hydroxyurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 1-methylthiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 1-methylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 1-prop-2-en-1-ylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 2-cyanoacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 2-methylpropanamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 3-oxobutanamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using acetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using butanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using carbamoylcarbamate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using dicarbonodithioimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using (E)-diazene-1,2-dicarboxamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using ethanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using ethanethioamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using ethyl acetate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using ethyl carbamoylcarbamate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using formamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using hexanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using hydrazinecarbothioamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using hydrazinecarboxamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using N-carbamothioylacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using N-hydroxydicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using N-nitrodicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using prop-2-enamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using propanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using sulfuric diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using thiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using urea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
0.04
-
using N-carbamoylacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
0.17
-
using 1-formylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
6.08
-
using dicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
8.9
-
using biuret as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(E)-diazene-1,2-dicarboxamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
(E)-diazene-1,2-dicarboxamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
1,1-dimethylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-carbamimidoylthiourea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-ethylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-formylurea + H2O
-
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-formylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-hydroxyurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-methylthiourea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-methylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-prop-2-en-1-ylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
2-cyanoacetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
2-methylpropanamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3-oxobutanamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
acetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
acetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
biuret + H2O
the enzyme acts uniquely on biuret physiologically
718459
Rhizobium leguminosarum bv. viciae
allophanic acid + NH3
-
-
-
ir
biuret + H2O
the enzyme acts uniquely on biuret physiologically
718459
Rhizobium leguminosarum bv. viciae 3841
allophanic acid + NH3
-
-
-
ir
butanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
carbamoylcarbamate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
dicarbonimidic diamide + H2O
-
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
dicarbonimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
dicarbonodithioimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
ethanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
ethanethioamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
ethyl acetate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
ethyl acetate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
ethyl carbamoylcarbamate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
formamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
hexanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
hydrazinecarbothioamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
hydrazinecarboxamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
N-carbamothioylacetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
N-carbamoylacetamide + H2O
-
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
N-carbamoylacetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
N-hydroxydicarbonimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
N-nitrodicarbonimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
prop-2-enamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
propanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
sulfuric diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
thiourea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
urea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
urea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 27121, calculated from amino acid sequence
Rhizobium leguminosarum bv. viciae
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4
-
Biuret
in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Rhizobium leguminosarum bv. viciae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
11
about 50% activity at pH 4.5 and pH 11.0
Rhizobium leguminosarum bv. viciae
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Rhizobium leguminosarum bv. viciae
calculated from amino acid sequence
-
5.7
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli DH5alpha cells
Rhizobium leguminosarum bv. viciae
General Stability (protein specific)
General Stability
Organism
potassium phosphate buffer yields optimal catalytic rates
Rhizobium leguminosarum bv. viciae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
N-ethylmaleimide
more than 90% loss of activity at 0.05 mM; more than 90% loss of activity at 0.5 mM
Rhizobium leguminosarum bv. viciae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.023
-
Biuret
in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
the addition of ZnSO4 or CoSO4 (0.05 mM and 1 mM) to enzyme assays gives only nominal increases in activity. No metal cofactors are detected
Rhizobium leguminosarum bv. viciae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27121
-
x * 27121, calculated from amino acid sequence
Rhizobium leguminosarum bv. viciae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
biuret + H2O
Rhizobium leguminosarum bv. viciae
the enzyme acts uniquely on biuret physiologically
allophanic acid + NH3
-
-
ir
biuret + H2O
Rhizobium leguminosarum bv. viciae 3841
the enzyme acts uniquely on biuret physiologically
allophanic acid + NH3
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
nickel affinity column chromatography
Rhizobium leguminosarum bv. viciae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.02
-
below 0.02 micromol/min/mg, using 1,1-dimethylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 1-carbamimidoylthiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 1-ethylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 1-hydroxyurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 1-methylthiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 1-methylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 1-prop-2-en-1-ylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 2-cyanoacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 2-methylpropanamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using 3-oxobutanamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using acetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using butanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using carbamoylcarbamate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using dicarbonodithioimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using (E)-diazene-1,2-dicarboxamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using ethanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using ethanethioamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using ethyl acetate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using ethyl carbamoylcarbamate as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using formamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using hexanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using hydrazinecarbothioamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using hydrazinecarboxamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using N-carbamothioylacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using N-hydroxydicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using N-nitrodicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using prop-2-enamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using propanediamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using sulfuric diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using thiourea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C; below 0.02 micromol/min/mg, using urea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
0.04
-
using N-carbamoylacetamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
0.17
-
using 1-formylurea as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
6.08
-
using dicarbonimidic diamide as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
8.9
-
using biuret as substrate, in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(E)-diazene-1,2-dicarboxamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
(E)-diazene-1,2-dicarboxamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
1,1-dimethylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-carbamimidoylthiourea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-ethylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-formylurea + H2O
-
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-formylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-hydroxyurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-methylthiourea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-methylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
1-prop-2-en-1-ylurea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
2-cyanoacetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
2-methylpropanamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
3-oxobutanamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
acetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
acetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
biuret + H2O
the enzyme acts uniquely on biuret physiologically
718459
Rhizobium leguminosarum bv. viciae
allophanic acid + NH3
-
-
-
ir
biuret + H2O
the enzyme acts uniquely on biuret physiologically
718459
Rhizobium leguminosarum bv. viciae 3841
allophanic acid + NH3
-
-
-
ir
butanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
carbamoylcarbamate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
dicarbonimidic diamide + H2O
-
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
dicarbonimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
dicarbonodithioimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
ethanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
ethanethioamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
ethyl acetate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
ethyl acetate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
ethyl carbamoylcarbamate + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
formamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
hexanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
hydrazinecarbothioamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
hydrazinecarboxamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
N-carbamothioylacetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
N-carbamoylacetamide + H2O
-
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
N-carbamoylacetamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
N-hydroxydicarbonimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
N-nitrodicarbonimidic diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
prop-2-enamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
propanediamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
sulfuric diamide + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
thiourea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
urea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae
?
-
-
-
?
urea + H2O
low specific activity
718459
Rhizobium leguminosarum bv. viciae 3841
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 27121, calculated from amino acid sequence
Rhizobium leguminosarum bv. viciae
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4
-
Biuret
in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Rhizobium leguminosarum bv. viciae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
11
about 50% activity at pH 4.5 and pH 11.0
Rhizobium leguminosarum bv. viciae
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Rhizobium leguminosarum bv. viciae
calculated from amino acid sequence
-
5.7
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
170
-
Biuret
in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
170
-
Biuret
in 0.1 M potassium phosphate buffer, pH 8.5, at 22C
Rhizobium leguminosarum bv. viciae
Other publictions for EC 3.5.1.84
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746233
Esquirol
Structural and biochemical ch ...
Rhizobium leguminosarum bv. viciae 3841, Rhizobium leguminosarum bv. viciae
PLoS One
13
e0192736
2018
-
-
1
1
9
-
-
9
-
-
-
2
-
2
-
-
1
-
-
-
-
-
4
-
-
-
-
8
-
-
-
-
-
-
-
-
-
1
-
1
9
-
-
-
-
9
-
-
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
8
-
-
-
-
-
1
1
-
9
9
718459
Cameron
-
New family of biuret hydrolase ...
Rhizobium leguminosarum bv. viciae, Rhizobium leguminosarum bv. viciae 3841
ACS Catal.
1
1075-1082
2011
-
-
1
-
-
1
1
1
-
1
1
2
-
2
-
-
1
-
-
-
5
-
43
1
-
-
-
1
1
1
-
-
-
1
-
-
-
1
-
-
-
1
-
1
-
1
-
1
1
2
-
-
-
1
-
-
5
-
43
1
-
-
-
1
1
1
-
1
-
-
-
-
1
1
246621
Karns
-
Genes encoding s-triazine degr ...
Klebsiella pneumoniae, Klebsiella pneumoniae 99
J. Agric. Food Chem.
45
1017-1022
1997
-
1
1
-
-
-
-
-
-
-
-
4
-
5
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246626
Cook
-
Biodegradation of s-triazine x ...
Gordonia rubripertincta, Gordonia rubripertincta DSM 10347 / NRRLB-15444R, Hormodendrum sp., Klebsiella pneumoniae, Klebsiella pneumoniae 90, Klebsiella pneumoniae 99, Penicillium spp., Pseudomonas sp., Sporothrix schenckii
FEMS Microbiol. Rev.
46
93-116
1987
-
2
-
-
-
-
-
-
-
-
-
6
-
12
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246620
Cook
Ring cleavage and degradative ...
Klebsiella pneumoniae, Klebsiella pneumoniae 99, Pseudomonas sp.
Biochem. J.
231
25-30
1985
-
-
-
-
-
5
3
-
-
-
-
8
-
6
-
-
1
-
-
-
2
1
8
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
3
-
-
-
-
-
8
-
-
-
1
-
-
2
1
8
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-