BRENDA - Enzyme Database
show all sequences of 3.5.1.84

Genes encoding s-triazine degradation are plasmid-borne in Klebsiella pneumoniae strain 99

Karns, J.S.; Eaton, R.W.; J. Agric. Food Chem. 45, 1017-1022 (1997)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
agriculture
one step in the mineralization of s-triazine compounds, extensive used as herbicides or pesticides in agriculture worldwide, and hence in their complete removal from environment
Klebsiella pneumoniae
Cloned(Commentary)
Commentary
Organism
strain 99, cloning large conjugal plasmid pPDL12, carrying the genes encoding the s-triazine degradation pathway, including the trzE gene encoding biuret amidohydrolase in Klebsiella planticola ATCC 33531, expression in Klebsiella planticola ATCC 33531
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
biuret + H2O
Klebsiella pneumoniae
imidodicarbonic diamide
urea + CO2 + NH3
-
-
?
biuret + H2O
Klebsiella pneumoniae
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
urea + CO2 + NH3
-
-
?
biuret + H2O
Klebsiella pneumoniae
involved in a pathway by which herbicides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
urea + CO2 + NH3
-
-
?
biuret + H2O
Klebsiella pneumoniae
second enzyme of degradative pathway of cyanuric acid
urea + CO2 + NH3
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella pneumoniae
-
wild-type and mutants
-
Klebsiella pneumoniae 99
-
wild-type and mutants
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
biuret + H2O
imidodicarbonic diamide
246621
Klebsiella pneumoniae
urea + CO2 + NH3
-
-
-
?
biuret + H2O
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
246621
Klebsiella pneumoniae
urea + CO2 + NH3
-
-
-
?
biuret + H2O
involved in a pathway by which herbicides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
246621
Klebsiella pneumoniae
urea + CO2 + NH3
-
-
-
?
biuret + H2O
second enzyme of degradative pathway of cyanuric acid
246621
Klebsiella pneumoniae
urea + CO2 + NH3
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
agriculture
one step in the mineralization of s-triazine compounds, extensive used as herbicides or pesticides in agriculture worldwide, and hence in their complete removal from environment
Klebsiella pneumoniae
Cloned(Commentary) (protein specific)
Commentary
Organism
strain 99, cloning large conjugal plasmid pPDL12, carrying the genes encoding the s-triazine degradation pathway, including the trzE gene encoding biuret amidohydrolase in Klebsiella planticola ATCC 33531, expression in Klebsiella planticola ATCC 33531
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
biuret + H2O
Klebsiella pneumoniae
imidodicarbonic diamide
urea + CO2 + NH3
-
-
?
biuret + H2O
Klebsiella pneumoniae
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
urea + CO2 + NH3
-
-
?
biuret + H2O
Klebsiella pneumoniae
involved in a pathway by which herbicides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
urea + CO2 + NH3
-
-
?
biuret + H2O
Klebsiella pneumoniae
second enzyme of degradative pathway of cyanuric acid
urea + CO2 + NH3
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
biuret + H2O
imidodicarbonic diamide
246621
Klebsiella pneumoniae
urea + CO2 + NH3
-
-
-
?
biuret + H2O
involved in metabolism of s-triazine ring structure, component of several important herbicides, including atrazine
246621
Klebsiella pneumoniae
urea + CO2 + NH3
-
-
-
?
biuret + H2O
involved in a pathway by which herbicides based on the symmetrical triazine ring, s-triazine, including atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, are degraded in bacteria
246621
Klebsiella pneumoniae
urea + CO2 + NH3
-
-
-
?
biuret + H2O
second enzyme of degradative pathway of cyanuric acid
246621
Klebsiella pneumoniae
urea + CO2 + NH3
-
-
-
?
Other publictions for EC 3.5.1.84
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746233
Esquirol
Structural and biochemical ch ...
Rhizobium leguminosarum bv. viciae 3841, Rhizobium leguminosarum bv. viciae
PLoS One
13
e0192736
2018
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1
1
9
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9
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2
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2
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1
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4
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8
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1
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1
9
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9
-
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2
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1
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-
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4
-
-
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8
-
-
-
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-
1
1
-
9
9
718459
Cameron
-
New family of biuret hydrolase ...
Rhizobium leguminosarum bv. viciae, Rhizobium leguminosarum bv. viciae 3841
ACS Catal.
1
1075-1082
2011
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1
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1
1
1
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1
1
2
-
2
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-
1
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-
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5
-
43
1
-
-
-
1
1
1
-
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-
1
-
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-
1
-
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-
1
-
1
-
1
-
1
1
2
-
-
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1
-
-
5
-
43
1
-
-
-
1
1
1
-
1
-
-
-
-
1
1
246621
Karns
-
Genes encoding s-triazine degr ...
Klebsiella pneumoniae, Klebsiella pneumoniae 99
J. Agric. Food Chem.
45
1017-1022
1997
-
1
1
-
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-
-
-
-
-
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4
-
5
-
-
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-
-
-
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4
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1
1
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4
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4
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-
246626
Cook
-
Biodegradation of s-triazine x ...
Gordonia rubripertincta, Gordonia rubripertincta DSM 10347 / NRRLB-15444R, Hormodendrum sp., Klebsiella pneumoniae, Klebsiella pneumoniae 90, Klebsiella pneumoniae 99, Penicillium spp., Pseudomonas sp., Sporothrix schenckii
FEMS Microbiol. Rev.
46
93-116
1987
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2
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6
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12
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6
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2
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6
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6
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246620
Cook
Ring cleavage and degradative ...
Klebsiella pneumoniae, Klebsiella pneumoniae 99, Pseudomonas sp.
Biochem. J.
231
25-30
1985
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5
3
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8
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6
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1
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2
1
8
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1
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5
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3
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8
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1
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2
1
8
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1
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