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Literature summary for 3.5.1.82 extracted from

  • Sakai, K.; Oshima, K.; Moriguchi, M.
    Purification and characterization of N-acyl-D-glutamate amidohydrolase from Pseudomonas sp. strain 5f-1 (1991), Appl. Environ. Microbiol., 57, 2540-2543.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.82

Activating Compound

Activating Compound Comment Organism Structure
acetate
-
 Pseudomonas sp.

General Stability

General Stability Organism
Na+, K+, Mg2+, Ba2+ or glycerol stabilizes Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Pseudomonas sp.
EDTA
-
 Pseudomonas sp.
Fe3+
-
 Pseudomonas sp.
Hg2+
-
 Pseudomonas sp.
Zn2+
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.67
-
 N-Acetyl-D-Glu
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 gel filtration Pseudomonas sp.
55000
-
 1 * 55000, SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas sp. the enzyme is inducibly produced by D-isomers of N-acetylglutamate, Glu, Asp, and Asn ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
589
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glycyl-D-Glu + H2O 0.79% of the activity with N-formyl-D-Glu Pseudomonas sp. Gly + D-Glu
-
 ?
additional information the enzyme is inducibly produced by D-isomers of N-acetylglutamate, Glu, Asp, and Asn Pseudomonas sp. ?
-
 ?
N-Acetyl-D-Glu + H2O 58% of the activity with N-formyl-D-Glu Pseudomonas sp. acetate + D-Glu
-
 ?
N-Butyl-D-Glu + H2O 21% of the activity with N-formyl-D-Glu Pseudomonas sp. Butanoate + D-Glu
-
 ?
N-Chloroacetyl-D-Glu + H2O 7.5% of the activity with N-formyl-D-Glu Pseudomonas sp. Chloroacetate + D-Glu
-
 ?
N-Formyl-D-Glu + H2O
-
 Pseudomonas sp. Formate + D-Glu
-
 ?
N-Propionyl-D-Glu + H2O 16% of the activity with N-formyl-D-Glu Pseudomonas sp. Propanoate + D-Glu
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
41
-
 10 min, in absence of a stabilizer, 50% loss of activity Pseudomonas sp.
48
-
 10 min, in presence of Mg2+, 50% loss of activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
9 9.5 45°C, quite stable, regardless of the presence or absence of MgCl2 Pseudomonas sp.