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Literature summary for 3.5.1.82 extracted from

  • Wakayama, M.; Miura, Y.; Oshima, K.; Sakai, K.; Moriguchi, M.
    Metal-characterization of N-acyl-D-glutamate amidohydrolase from Pseudomonas sp. strain 5f-1 (1995), Biosci. Biotechnol. Biochem., 59, 1489-1492.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline rapid inactivation even at an alkaline pH, 9.3 Pseudomonas sp.
Cu2+
-
 Pseudomonas sp.
EDTA reactivation: Zn2+, 66%, Co2+, 40%, or Mn2+ 9% Pseudomonas sp.
Fe2+
-
 Pseudomonas sp.
Zn2+
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc contains 2.06-2.61 gatom of Zn per mol of enzyme. The zinc atom is required for the catalytic activity and stability Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 gel filtration Pseudomonas sp.
55000
-
 1 * 55000, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. 5f-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-Acetyl-D-Glu + H2O
-
 Pseudomonas sp. acetate + D-Glu
-
 ?
N-Acetyl-D-Glu + H2O
-
 Pseudomonas sp. 5f-1 acetate + D-Glu
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 Zn2+ increases heat stability. Thermal stability increases about 10°C in the presence of Zn2+ or Co2+. The optimal concentration for Zn2+ at 45°C and pH 6.5 is 0.03-0.05 mM. The optimal concentration for Co2+ at 45°C, pH 6.5 is 1.0-2.0 mM Pseudomonas sp.
45
-
 10 min, in absence of added metal ions, 60-70% loss of activity Pseudomonas sp.