Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.81 extracted from

  • Wakayama, M.; Kitahata, S.; Manoch, L.; Tachiki, T.; Yoshimune, K.; Moriguchi, M.
    Production, purification and properties of D-aminoacylase from a newly isolated Trichoderma sp. SKW-36 (2004), Process Biochem., 39, 1119-1124.
No PubMed abstract available
Search for more literature for 3.5.1.81

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is not inducible by N-acyl-D-amino acids and D-amino acid Trichoderma sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.89
-
 N-acetyl-D-methionine pH 8.0, 50°C Trichoderma sp.
2.04
-
 N-acetyl-D-leucine pH 8.0, 50°C Trichoderma sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 gel filtration Trichoderma sp.

Organism

Organism UniProt Comment Textmining
Trichoderma sp.
-
 isolated from soil, constitutive enzyme
-
Trichoderma sp. SKW-36
-
 isolated from soil, constitutive enzyme
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration Trichoderma sp.

Source Tissue

Source Tissue Comment Organism Textmining
additional information constitutive enzyme Trichoderma sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
42.1
-
 purified enzyme Trichoderma sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-leucine + H2O
-
 Trichoderma sp. acetate + D-leucine
-
 ?
N-acetyl-D-leucine + H2O
-
 Trichoderma sp. SKW-36 acetate + D-leucine
-
 ?
N-acetyl-D-methionine + H2O
-
 Trichoderma sp. acetate + D-methionine
-
 ?
N-acetyl-D-methionine + H2O
-
 Trichoderma sp. SKW-36 acetate + D-methionine
-
 ?
N-acetyl-D-phenylalanine + H2O
-
 Trichoderma sp. acetate + D-phenylalanine
-
 ?
N-acetyl-D-phenylalanine + H2O
-
 Trichoderma sp. SKW-36 acetate + D-phenylalanine
-
 ?
N-acetyl-D-valine + H2O
-
 Trichoderma sp. acetate + D-valine
-
 ?
N-acetyl-D-valine + H2O
-
 Trichoderma sp. SKW-36 acetate + D-valine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Trichoderma sp.

Synonyms

Synonyms Comment Organism
D-Aminoacylase
-
 Trichoderma sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Trichoderma sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified enzyme, fully stable up to 40°C Trichoderma sp.
60
-
 purified enzyme, inactivation Trichoderma sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Trichoderma sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 purified enzyme, fully stable Trichoderma sp.

pI Value

Organism Comment pI Value Maximum pI Value
Trichoderma sp.
-
-
 4.5