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Literature summary for 3.5.1.81 extracted from

  • Kameda, Y.; Hase, T.; Kanatomo, S.; Kita, Y.
    Studies on acylase activity and micro-organisms. XXVL. Purification and properties of D-acylase (N-acyl-D-amino-acid amidohydrolase) from AAA 6029 (Pseudomonas sp.) (1978), Chem. Pharm. Bull., 26, 2698-2704.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
L-Cys slight stimulation Pseudomonas sp.

General Stability

General Stability Organism
not stabilized in presence of 10% glycerol Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
 Pseudomonas sp.
PCMB 1 mM Pseudomonas sp.
Zn2+
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. AAA 6029
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40.6
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-Acetyl-D-Ala + H2O
-
 Pseudomonas sp. acetate + D-Ala
-
 ?
N-Acetyl-D-Ala + H2O
-
 Pseudomonas sp. AAA 6029 acetate + D-Ala
-
 ?
N-acetyl-D-Leu + H2O
-
 Pseudomonas sp. acetate + D-Leu
-
 ?
N-acetyl-D-Leu + H2O
-
 Pseudomonas sp. AAA 6029 acetate + D-Leu
-
 ?
N-Acetyl-D-Met + H2O
-
 Pseudomonas sp. acetate + D-Met
-
 ?
N-Acetyl-D-Met + H2O
-
 Pseudomonas sp. AAA 6029 acetate + D-Met
-
 ?
N-Acetyl-D-Phe + H2O
-
 Pseudomonas sp. acetate + D-Phe
-
 ?
N-Acetyl-D-Phe + H2O
-
 Pseudomonas sp. AAA 6029 acetate + D-Phe
-
 ?
N-Benzoyl-Ala + H2O
-
 Pseudomonas sp. Benzoate + Ala
-
 ?
N-Benzoyl-D-Leu + H2O
-
 Pseudomonas sp. Benzoate + D-Leu
-
 ?
N-Benzoyl-D-Met + H2O
-
 Pseudomonas sp. Benzoate + D-Met
-
 ?
N-Benzoyl-D-Phe + H2O
-
 Pseudomonas sp. Benzoate + D-Phe
-
 ?
N-Benzoyl-D-Phe + H2O
-
 Pseudomonas sp. AAA 6029 Benzoate + D-Phe
-
 ?
N-Benzoyl-Val + H2O
-
 Pseudomonas sp. Benzoate + Val
-
 ?
N-Formyl-D-Phe + H2O
-
 Pseudomonas sp. Formate + D-Phe
-
 ?
N-Phenylacetyl-D-Phe + H2O
-
 Pseudomonas sp. Phenylacetate + D-Phe
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 pH 8.0, 5 min, stable up to Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 N-acetyl-D-Phe, N-formyl-D-Phe Pseudomonas sp.
7.5
-
 N-phenylacetyl-D-Phe Pseudomonas sp.
10.5
-
 N-phenylacetyl-D-Phe Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 4°C, 48 h, relatively stable between pH 7 and pH 9 Pseudomonas sp.