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Literature summary for 3.5.1.77 extracted from
- Improving the functional expression of N-carbamoylase by directed evolution using the green fluorescent protein fusion reporter system (2004), Methods Enzymol., 388, 187-195.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| T262A | 1.5fold increased activity, improvement in expression levels, mutation results in a significant improvement in both oxidative stability and thermostability, increases stability of N-carbamoylase in vivo, thereby preventing it from degradation by cellular proteases | Agrobacterium tumefaciens |
| V40A | 2fold increased activity, improvement in expression levels, mutation mainly gives rise to the increase in oxidative stability rather than thermostability | Agrobacterium tumefaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium tumefaciens | - |
NRRL B11291 | - |
| Agrobacterium tumefaciens NRRL B11291 | - |
NRRL B11291 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Agrobacterium tumefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-carbamoyl-D-hydroxyphenylglycine + H2O | - |
Agrobacterium tumefaciens | D-hydroxyphenylglycine + NH3 + CO2 | - |
? |  |
| N-carbamoyl-D-hydroxyphenylglycine + H2O | - |
Agrobacterium tumefaciens NRRL B11291 | D-hydroxyphenylglycine + NH3 + CO2 | - |
? | |
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Release 2023.1 (January 2023)
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