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Literature summary for 3.5.1.77 extracted from

  • Wu, S.; Liu, Y.; Zhao, G.; Wang, J.; Sun, W.
    Thermostable D-carbamoylase from Sinorhizobium morelens S-5: purification, characterization and gene expression in Escherichia coli (2006), Biochimie, 88, 237-244.
    View publication on PubMed
Search for more literature for 3.5.1.77

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ensifer adhaerens

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 2 mM, 97.6% inhibition Ensifer adhaerens
Ba2+ 2 mM, 8% inhibition Ensifer adhaerens
Co2+ 2 mM, 67% inhibition Ensifer adhaerens
Cu2+ 2 mM, 98.4% inhibition Ensifer adhaerens
H2O2 32% loss of activity after treatment with 0.1 mM H2O2 for 30 min, 57% loss of activity after treatment with 0.2 mM H2O2 for 30 min Ensifer adhaerens
Mg2+ 2 mM, 24.6% inhibition Ensifer adhaerens
Mn2+ 2 mM, 58% inhibition Ensifer adhaerens
Sn2+ 2 mM, 46.5% inhibition Ensifer adhaerens
Zn2+ 2 mM, 98% inhibition Ensifer adhaerens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.76
-
 N-carbamoyl-D-p-hydroxyphenylglycine pH 7.0, 40°C Ensifer adhaerens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 2 mM, enhances activity to about 110% Ensifer adhaerens
Fe2+ 2 mM, enhances activity to about 110% Ensifer adhaerens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34363
-
 4 * 34363, calculated from sequence Ensifer adhaerens
38000
-
 4 * 38000, SDS-PAGE Ensifer adhaerens
150000
-
 gel filtration Ensifer adhaerens

Organism

Organism UniProt Comment Textmining
Ensifer adhaerens Q5S260
-
-
Ensifer adhaerens S-5 Q5S260
-
-

Oxidation Stability

Oxidation Stability Organism
high oxidative stability Ensifer adhaerens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Ensifer adhaerens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.25
-
 enzyme from Sinorhizobium morelens Ensifer adhaerens
21.4
-
 enzyme recombinantly expressed in Escherichia coli Ensifer adhaerens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information strict specificity towards N-carbamoyl-D-amino acids, no activity with N-carbamoyl-L-amino acids Ensifer adhaerens ?
-
 ?
additional information strict specificity towards N-carbamoyl-D-amino acids, no activity with N-carbamoyl-L-amino acids Ensifer adhaerens S-5 ?
-
 ?
N-carbamoyl-D-Ala + H2O 13% of activity with N-carbamoyl-D-p-hydroxyphenylglycine Ensifer adhaerens D-Ala + NH3 + CO2
-
 ?
N-carbamoyl-D-Ala + H2O 13% of activity with N-carbamoyl-D-p-hydroxyphenylglycine Ensifer adhaerens S-5 D-Ala + NH3 + CO2
-
 ?
N-carbamoyl-D-Leu + H2O 43% of activity with N-carbamoyl-D-p-hydroxyphenylglycine Ensifer adhaerens D-Leu + NH3 + CO2
-
 ?
N-carbamoyl-D-Leu + H2O 43% of activity with N-carbamoyl-D-p-hydroxyphenylglycine Ensifer adhaerens S-5 D-Leu + NH3 + CO2
-
 ?
N-carbamoyl-D-Met + H2O 48% of activity with N-carbamoyl-D-p-hydroxyphenylglycine Ensifer adhaerens D-Met + NH3 + CO2
-
 ?
N-carbamoyl-D-p-hydroxyphenylglycine + H2O
-
 Ensifer adhaerens D-p-hydroxyphenylglycine + NH3 + CO2
-
 ?
N-carbamoyl-D-p-hydroxyphenylglycine + H2O
-
 Ensifer adhaerens S-5 D-p-hydroxyphenylglycine + NH3 + CO2
-
 ?
N-carbamoyl-D-phenylglycine + H2O 58% of activity with N-carbamoyl-D-p-hydroxyphenylglycine Ensifer adhaerens D-phenylglycine + NH3 + CO2
-
 ?
N-carbamoyl-D-phenylglycine + H2O 58% of activity with N-carbamoyl-D-p-hydroxyphenylglycine Ensifer adhaerens S-5 D-phenylglycine + NH3 + CO2
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 38000, SDS-PAGE Ensifer adhaerens
tetramer 4 * 34363, calculated from sequence Ensifer adhaerens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Ensifer adhaerens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 65 45°C: about 50% of maximal activity, 65°C: about 75% of maximal activity Ensifer adhaerens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 7.0, 30 min, stable up to Ensifer adhaerens
55
-
 pH 7.0, 30 min, 10% loss of activity Ensifer adhaerens
60
-
 pH 7.0, 30 min, about 50% loss of activity Ensifer adhaerens
65
-
 pH 7.0, 30 min, about 90% of maximal activity Ensifer adhaerens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Ensifer adhaerens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5 pH 6.5: about 90% of maximal activity, pH 7.5: about 95% of maximal activity Ensifer adhaerens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8.3 30 min, 30 min, stable Ensifer adhaerens