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Literature summary for 3.5.1.77 extracted from

  • Chen, C.Y.; Chiu, W.C.; Liu, J.S.; Hsu, W.H.; Wang, W.C.
    Structural basis for catalysis and substrate specificity of Agrobacterium radiobacter N-carbamoyl-D-amino acid amidohydrolase (2003), J. Biol. Chem., 278, 26194-26201.
    View publication on PubMed
Search for more literature for 3.5.1.77

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor-diffusion method. Crystal structure of mutant enzymes C172A, C172S, R175A, N173A and the complexes of mutant enzymes R175A and N173A with the substrate N-carbamoyl-D-p-hydroxyphenylglycine Agrobacterium tumefaciens

Protein Variants

Protein Variants Comment Organism
C172A inactive mutant enzyme Agrobacterium tumefaciens
C172S less than 0.1% of the wild-type enzyme Agrobacterium tumefaciens
N173A KM-value for N-carbamoyl-D-p-hydroxyphenylglycine is 40% of the wild-type value. The ratio of turnover number to Km-value for N-carbamoyl-D-p-hydroxyphenylglycine is 5.5fold lower than wild-type ratio. Tm is 68°C compared to 63° for wild-type enzyme Agrobacterium tumefaciens
R175A inactive mutant enzyme Agrobacterium tumefaciens
R175K KM-value for N-carbamoyl-D-p-hydroxyphenylglycine is 2.5fold higher than the wild-type value. The ratio of turnover number to Km-value for N-carbamoyl-D-p-hydroxyphenylglycine is 4706fold lower than wild-type ratio. Tm is 65°C compared to 63° for wild-type enzyme Agrobacterium tumefaciens
R176A inactive mutant enzyme Agrobacterium tumefaciens
R176K KM-value for N-carbamoyl-D-p-hydroxyphenylglycine is 4.2fold higher than the wild-type value. The ratio of turnover number to Km-value for N-carbamoyl-D-p-hydroxyphenylglycine is 364fold lower than wild-type ratio. Tm is 65°C compared to 63° for wild-type enzyme Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
 N-carbamoyl-D-p-hydroxyphenylglycine pH 7.0, 37°C, mutant enzyme N173A Agrobacterium tumefaciens
1.3
-
 N-carbamoyl-D-p-hydroxyphenylglycine pH 7.0, 37°C, wild-type enzyme Agrobacterium tumefaciens
3.2
-
 N-carbamoyl-D-p-hydroxyphenylglycine pH 7.0, 37°C, mutant enzyme R175K Agrobacterium tumefaciens
5.4
-
 N-carbamoyl-D-p-hydroxyphenylglycine pH 7.0, 37°C, mutant enzyme R176K Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 x * 36000, wild-type enzyme and mutant enzymes C172S, C172A, R175A, R175K, R176A, R176K and N173A Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbamoyl-D-p-hydroxyphenylglycine + H2O
-
 Agrobacterium tumefaciens D-p-hydroxyphenylglycine + NH3 + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 36000, wild-type enzyme and mutant enzymes C172S, C172A, R175A, R175K, R176A, R176K and N173A Agrobacterium tumefaciens