Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is suitable for use in the production of D-amino acids | Agrobacterium sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Agrobacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Agrobacterium sp. | |
Cu2+ | - |
Agrobacterium sp. | |
Hg2+ | - |
Agrobacterium sp. | |
NH4+ | - |
Agrobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34285 | - |
2 * 34285, calculation from nucleotide sequence | Agrobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium sp. | - |
- |
- |
Agrobacterium sp. KNK712 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Agrobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbamoyl-D-Ala + H2O | 81% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. | D-Ala + NH3 + CO2 | - |
? | |
N-carbamoyl-D-Ala + H2O | 81% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. KNK712 | D-Ala + NH3 + CO2 | - |
? | |
N-carbamoyl-D-Asp + H2O | 81% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. | D-Asp + NH3 + CO2 | - |
? | |
N-carbamoyl-D-Ile + H2O | 72% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. | D-Ile + NH3 + CO2 | - |
? | |
N-carbamoyl-D-Leu + H2O | as active as with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. | D-Leu + NH3 + CO2 | - |
? | |
N-carbamoyl-D-Leu + H2O | as active as with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. KNK712 | D-Leu + NH3 + CO2 | - |
? | |
N-carbamoyl-D-p-hydroxyphenylglycine + H2O | - |
Agrobacterium sp. | D-p-hydroxyphenylglycine + NH3 + CO2 | - |
? | |
N-carbamoyl-D-Phe + H2O | 53% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. | D-Phe + NH3 + CO2 | - |
? | |
N-carbamoyl-D-Phe + H2O | 53% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. KNK712 | D-Phe + NH3 + CO2 | - |
? | |
N-carbamoyl-D-phenylglycine + H2O | 110% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. | D-phenylglycine + NH3 + CO2 | - |
? | |
N-carbamoyl-D-Ser + H2O | 55% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. | D-Ser + NH3 + CO2 | - |
? | |
N-carbamoyl-D-Ser + H2O | 55% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. KNK712 | D-Ser + NH3 + CO2 | - |
? | |
N-carbamoyl-D-Trp + H2O | 42% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. | D-Trp + NH3 + CO2 | - |
? | |
N-carbamoyl-D-Val + H2O | 38% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. | D-Val + NH3 + CO2 | - |
? | |
N-carbamoyl-D-Val + H2O | 38% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. KNK712 | D-Val + NH3 + CO2 | - |
? | |
N-Carbamoyl-DL-2-amino-n-butyric acid + H2O | 110% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. | ? | - |
? | |
N-Carbamoyl-DL-Met + H2O | 160% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. | ? | - |
? | |
N-Carbamoyl-DL-norleucine + H2O | 105% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. | ? | - |
? | |
N-Carbamoyl-DL-norvaline + H2O | 76% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. | ? | - |
? | |
N-Carbamoyl-DL-Thr + H2O | 27% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. | ? | - |
? | |
N-Carbamoyl-Gly + H2O | 12% of the activity with N-carbamoyl-D-p-hydroxyphenylglycine | Agrobacterium sp. | Gly + NH3 + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34285, calculation from nucleotide sequence | Agrobacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Agrobacterium sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | 40°C: about 50% of maximal activity, 70°C: about 70% of maximal activity | Agrobacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
pH 7.0, 10 min, stable | Agrobacterium sp. |
60 | - |
pH 7.0, 10 min, about 25% loss of activity | Agrobacterium sp. |
65 | - |
pH 7.0, 10 min, complete loss of activity | Agrobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Agrobacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.2 | pH 6.0: about 50% of maximal activity, pH 8.2: about 65% loss of maximal activity | Agrobacterium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
40°C, 5 h, stable | Agrobacterium sp. |