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show all sequences of 3.5.1.73

Purification and characterisation of a microbial L-carnitine amidase

Joeres, U.; Kula, M.R.; Appl. Microbiol. Biotechnol. 40, 606-610 (1994)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
10 mM, 18% loss of activity
Bacteria
5,5'-dithiobis(2-nitrobenzoate)
1 mM, 66% loss of activity
Bacteria
CoCl2
10 mM, 24% loss of activity
Bacteria
EDTA
10 mM, 27% loss of activity
Bacteria
FeCl2
10 mM, 43% loss of activity
Bacteria
glutathione
10 mM, 28% loss of activity
Bacteria
HgCl2
1 mM, complete loss of activity
Bacteria
iodoacetate
1 mM, 28% loss of activity
Bacteria
KCN
10 mM, 12% loss of activity
Bacteria
MnCl2
10 mM, 24% loss of activity
Bacteria
p-hydroxymercuribenzoate
1 mM, 49% loss of activity
Bacteria
phenylmethanesulfonyl fluoride
1 mM, 77% loss of activity
Bacteria
ZnCl2
1 mM, 94% loss of activity
Bacteria
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
11.6
-
L-Carnitine amide
-
Bacteria
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
66000
-
2 * 66000, SDS-PAGE
Bacteria
125000
-
gel filtration
Bacteria
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacteria
-
DSM 6230, unidentified
-
Purification (Commentary)
Commentary
Organism
-
Bacteria
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
328
-
-
Bacteria
Storage Stability
Storage Stability
Organism
-20°C, stable for several months
Bacteria
25°C, in absence of substrate, half-life is 2.6 days
Bacteria
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-carnitine amide + H2O
-
288939
Bacteria
L-carnitine + NH3
-
288939
Bacteria
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 66000, SDS-PAGE
Bacteria
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Bacteria
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
50
about 40% of maximal activity at 20°C and at 50°C
Bacteria
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
-
in absence of substrate, half-life: 2.6 days
Bacteria
30
-
stable up to
Bacteria
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Bacteria
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10
pH 6.0: about 40% of maximal activity, pH 10.0: about 35% of maximal activity
Bacteria
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
9.5
30°C, 30 min, less than 20% loss of activity in the pH-range 5.0-9.5
Bacteria
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
10 mM, 18% loss of activity
Bacteria
5,5'-dithiobis(2-nitrobenzoate)
1 mM, 66% loss of activity
Bacteria
CoCl2
10 mM, 24% loss of activity
Bacteria
EDTA
10 mM, 27% loss of activity
Bacteria
FeCl2
10 mM, 43% loss of activity
Bacteria
glutathione
10 mM, 28% loss of activity
Bacteria
HgCl2
1 mM, complete loss of activity
Bacteria
iodoacetate
1 mM, 28% loss of activity
Bacteria
KCN
10 mM, 12% loss of activity
Bacteria
MnCl2
10 mM, 24% loss of activity
Bacteria
p-hydroxymercuribenzoate
1 mM, 49% loss of activity
Bacteria
phenylmethanesulfonyl fluoride
1 mM, 77% loss of activity
Bacteria
ZnCl2
1 mM, 94% loss of activity
Bacteria
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
11.6
-
L-Carnitine amide
-
Bacteria
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
66000
-
2 * 66000, SDS-PAGE
Bacteria
125000
-
gel filtration
Bacteria
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bacteria
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
328
-
-
Bacteria
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable for several months
Bacteria
25°C, in absence of substrate, half-life is 2.6 days
Bacteria
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-carnitine amide + H2O
-
288939
Bacteria
L-carnitine + NH3
-
288939
Bacteria
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 66000, SDS-PAGE
Bacteria
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Bacteria
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
50
about 40% of maximal activity at 20°C and at 50°C
Bacteria
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
-
in absence of substrate, half-life: 2.6 days
Bacteria
30
-
stable up to
Bacteria
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Bacteria
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10
pH 6.0: about 40% of maximal activity, pH 10.0: about 35% of maximal activity
Bacteria
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
9.5
30°C, 30 min, less than 20% loss of activity in the pH-range 5.0-9.5
Bacteria
Other publictions for EC 3.5.1.73
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
288941
Kula
-
New microbial amidases ...
Bacteria
Ann. N. Y. Acad. Sci.
799
725-728
1996
-
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288940
Joeres
-
Studies on the kinetics and ap ...
Bacteria
Biocatal. Biotransform.
12
27-36
1995
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288939
Joeres
Purification and characterisat ...
Bacteria
Appl. Microbiol. Biotechnol.
40
606-610
1994
-
-
-
-
-
-
13
1
-
-
2
-
-
1
-
-
1
-
-
-
1
2
1
1
1
1
2
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
13
-
1
-
-
2
-
-
-
-
1
-
-
1
2
1
1
1
1
2
-
1
1
1
-
-
-
-
-
-
-