BRENDA - Enzyme Database
show all sequences of 3.5.1.69

Comprehensive characterization of sphingolipid ceramide N-deacylase for the synthesis and fatty acid remodeling of glycosphingolipids

Han, Y.B.; Wu, L.; Rich, J.R.; Huang, F.T.; Withers, S.G.; Feng, Y.; Yang, G.Y.; Appl. Microbiol. Biotechnol. 99, 6715-6726 (2015)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Triton X-100
activates the hydrolytic reaction, best at 0.5% w/v, but inhibits the synthetic reaction
Shewanella algae
Application
Application
Commentary
Organism
synthesis
the enzyme can serve as a biocatalyst in the enzymatic synthesis of glycosphingolipids since it catalyzes the reversible hydrolysis/synthesis of the amide linkage between the fatty acid and the sphingosine base in the ceramide moiety of glycosphingolipids
Pseudomonas sp.
synthesis
the enzyme can serve as a biocatalyst in the enzymatic synthesis of glycosphingolipids since it catalyzes the reversible hydrolysis/synthesis of the amide linkage between the fatty acid and the sphingosine base in the ceramide moiety of glycosphingolipids
Shewanella algae
Cloned(Commentary)
Cloned (Commentary)
Organism
the gene encoding the mature SA_SCD protein, which lacks its 38-residue N-terminal secretion signal sequence and from which the 277-residue C-terminal sequence is deleted, is codon-optimized and expressed in Escherichia coli strain BL21 (DE3) pLysS as Histagged enzyme
Shewanella algae
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Co2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Cu2+
inhibits the hydrolytic activity and the synthetic activity of the enzyme
Shewanella algae
deoxycholic acid
complete inhibition of the hydrolytic and synthetic activity
Shewanella algae
dimethoxyethane
addition of 5% of DME strongly promoted the activity, further increasing the DME concentrations progressively inhibited synthetic activity
Shewanella algae
DMSO
low concentrations of DMSO, up to a maximum at 10%, enhance synthetic activity, while higher concentrations inhibit
Shewanella algae
Fe2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Mg2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Mn2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Ni2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
taurodeoxycholate
complete inhibition of the hydrolytic and synthetic activity
Shewanella algae
Triton X-100
activates the hydrolytic reaction, best at 0.5% w/v, but inhibits the synthetic reaction
Shewanella algae
Zn2+
inhibits the hydrolytic activity and the synthetic activity of the enzyme
Shewanella algae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Co2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Fe2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Mg2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Mn2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
additional information
poor effect by EDTA
Shewanella algae
Ni2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas sp.
-
-
-
Shewanella algae
Q8RTY6
-
-
Shewanella algae G8
Q8RTY6
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant His-tagged enzyme, lacking the secretion signal, from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography
Shewanella algae
Source Tissue
Source Tissue
Commentary
Organism
Textmining
commercial preparation
-
Pseudomonas sp.
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
16-(acetylsulfanyl)hexadecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
16-(acetylsulfanyl)hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
16-(acetylsulfanyl)hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
16-[(prop-2-yn-1-yl)oxy]hexadecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
16-[(prop-2-yn-1-yl)oxy]hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
16-[(prop-2-yn-1-yl)oxy]hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
2-hydroxydodecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
2-hydroxydodecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
2-hydroxydodecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
2-hydroxyhexadecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
2-hydroxyhexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
2-hydroxyhexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
arachidic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
arachidic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
behenic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
behenic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
caproic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
caproic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
caprylic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
caprylic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
decanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
decanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
docosahexaenoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
docosahexaenoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
eicosapentaenoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
eicosapentaenoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
GM1a + H2O
-
733197
Pseudomonas sp.
stearic acid + lyso-GM1a
-
-
-
r
GM1a + H2O
-
733197
Shewanella algae
stearic acid + lyso-GM1a
-
-
-
r
GM3 + H2O
-
733197
Pseudomonas sp.
stearic acid + lyso-GM3
-
-
-
r
GM3 + H2O
-
733197
Shewanella algae
stearic acid + lyso-GM3
-
-
-
r
hexadecanedioic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
hexadecanedioic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
hexadecanedioic acid methyl ester + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
hexadecanedioic acid methyl ester + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
lauric acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
lauric acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
lignoceric acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
lignoceric acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
additional information
the enzyme catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. PS_SCD also prefers glycosphingolipids possessing larger sugar moieties, but it does not show preference towards glycosphingolipids with charged head groups, the enzyme hydrolyzes sulfatide faster than GM1a and GM3, specificity of the hydrolytic and synthetic reactions of PS_SCD for hydrophilic head groups, overview
733197
Pseudomonas sp.
?
-
-
-
?
additional information
the enzyme catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. The enzyme shows high catalytic efficiency and has a very broad substrate specificity both in hydrolysis and synthesis, especially for unsaturated fatty acids and fatty acids with very short or long acyl chains. The enzyme SA_SCD hydrolyzes GM1a and GM3 fast, specificity of the hydrolytic and synthetic reactions of SA_SCD for hydrophilic head groups, overview
733197
Shewanella algae
?
-
-
-
?
additional information
the enzyme catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. The enzyme shows high catalytic efficiency and has a very broad substrate specificity both in hydrolysis and synthesis, especially for unsaturated fatty acids and fatty acids with very short or long acyl chains. The enzyme SA_SCD hydrolyzes GM1a and GM3 fast, specificity of the hydrolytic and synthetic reactions of SA_SCD for hydrophilic head groups, overview
733197
Shewanella algae G8
?
-
-
-
?
myristic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
myristic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
oleic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
oleic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
omega-azido palmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
omega-azido palmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
omega-bromo-palmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
omega-bromo-palmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
omega-hydroxypalmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
omega-hydroxypalmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
palmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
palmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
PS_SCD
-
Pseudomonas sp.
SA_SCD
-
Shewanella algae
SCDase
-
Pseudomonas sp.
SCDase
-
Shewanella algae
sphingolipid ceramide N-deacylase
-
Pseudomonas sp.
sphingolipid ceramide N-deacylase
-
Shewanella algae
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Pseudomonas sp.
37
-
assay at
Shewanella algae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at, forward direction
Pseudomonas sp.
6
-
hydrolytic activity optimum
Shewanella algae
7.5
-
assay at, reverse direction
Pseudomonas sp.
7.5
-
synthetic activity optimum
Shewanella algae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
9
activity range, hydrolysis
Shewanella algae
5.5
10
activity range, synthesis
Shewanella algae
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Triton X-100
activates the hydrolytic reaction, best at 0.5% w/v, but inhibits the synthetic reaction
Shewanella algae
Application (protein specific)
Application
Commentary
Organism
synthesis
the enzyme can serve as a biocatalyst in the enzymatic synthesis of glycosphingolipids since it catalyzes the reversible hydrolysis/synthesis of the amide linkage between the fatty acid and the sphingosine base in the ceramide moiety of glycosphingolipids
Pseudomonas sp.
synthesis
the enzyme can serve as a biocatalyst in the enzymatic synthesis of glycosphingolipids since it catalyzes the reversible hydrolysis/synthesis of the amide linkage between the fatty acid and the sphingosine base in the ceramide moiety of glycosphingolipids
Shewanella algae
Cloned(Commentary) (protein specific)
Commentary
Organism
the gene encoding the mature SA_SCD protein, which lacks its 38-residue N-terminal secretion signal sequence and from which the 277-residue C-terminal sequence is deleted, is codon-optimized and expressed in Escherichia coli strain BL21 (DE3) pLysS as Histagged enzyme
Shewanella algae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Co2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Cu2+
inhibits the hydrolytic activity and the synthetic activity of the enzyme
Shewanella algae
deoxycholic acid
complete inhibition of the hydrolytic and synthetic activity
Shewanella algae
dimethoxyethane
addition of 5% of DME strongly promoted the activity, further increasing the DME concentrations progressively inhibited synthetic activity
Shewanella algae
DMSO
low concentrations of DMSO, up to a maximum at 10%, enhance synthetic activity, while higher concentrations inhibit
Shewanella algae
Fe2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Mg2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Mn2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Ni2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
taurodeoxycholate
complete inhibition of the hydrolytic and synthetic activity
Shewanella algae
Triton X-100
activates the hydrolytic reaction, best at 0.5% w/v, but inhibits the synthetic reaction
Shewanella algae
Zn2+
inhibits the hydrolytic activity and the synthetic activity of the enzyme
Shewanella algae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Co2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Fe2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Mg2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Mn2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
additional information
poor effect by EDTA
Shewanella algae
Ni2+
promotes the hydrolytic activity but inhibits the synthetic activity of the enzyme
Shewanella algae
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme, lacking the secretion signal, from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography
Shewanella algae
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
commercial preparation
-
Pseudomonas sp.
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
16-(acetylsulfanyl)hexadecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
16-(acetylsulfanyl)hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
16-(acetylsulfanyl)hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
16-[(prop-2-yn-1-yl)oxy]hexadecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
16-[(prop-2-yn-1-yl)oxy]hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
16-[(prop-2-yn-1-yl)oxy]hexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
2-hydroxydodecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
2-hydroxydodecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
2-hydroxydodecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
2-hydroxyhexadecanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
2-hydroxyhexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
2-hydroxyhexadecanoic acid + lyso-GM1a
-
733197
Shewanella algae G8
? + H2O
-
-
-
r
arachidic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
arachidic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
behenic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
behenic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
caproic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
caproic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
caprylic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
caprylic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
decanoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
decanoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
docosahexaenoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
docosahexaenoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
eicosapentaenoic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
eicosapentaenoic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
GM1a + H2O
-
733197
Pseudomonas sp.
stearic acid + lyso-GM1a
-
-
-
r
GM1a + H2O
-
733197
Shewanella algae
stearic acid + lyso-GM1a
-
-
-
r
GM3 + H2O
-
733197
Pseudomonas sp.
stearic acid + lyso-GM3
-
-
-
r
GM3 + H2O
-
733197
Shewanella algae
stearic acid + lyso-GM3
-
-
-
r
hexadecanedioic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
hexadecanedioic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
hexadecanedioic acid methyl ester + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
hexadecanedioic acid methyl ester + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
lauric acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
lauric acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
lignoceric acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
lignoceric acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
additional information
the enzyme catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. PS_SCD also prefers glycosphingolipids possessing larger sugar moieties, but it does not show preference towards glycosphingolipids with charged head groups, the enzyme hydrolyzes sulfatide faster than GM1a and GM3, specificity of the hydrolytic and synthetic reactions of PS_SCD for hydrophilic head groups, overview
733197
Pseudomonas sp.
?
-
-
-
?
additional information
the enzyme catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. The enzyme shows high catalytic efficiency and has a very broad substrate specificity both in hydrolysis and synthesis, especially for unsaturated fatty acids and fatty acids with very short or long acyl chains. The enzyme SA_SCD hydrolyzes GM1a and GM3 fast, specificity of the hydrolytic and synthetic reactions of SA_SCD for hydrophilic head groups, overview
733197
Shewanella algae
?
-
-
-
?
additional information
the enzyme catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. The enzyme shows high catalytic efficiency and has a very broad substrate specificity both in hydrolysis and synthesis, especially for unsaturated fatty acids and fatty acids with very short or long acyl chains. The enzyme SA_SCD hydrolyzes GM1a and GM3 fast, specificity of the hydrolytic and synthetic reactions of SA_SCD for hydrophilic head groups, overview
733197
Shewanella algae G8
?
-
-
-
?
myristic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
myristic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
oleic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
oleic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
omega-azido palmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
omega-azido palmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
omega-bromo-palmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
omega-bromo-palmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
omega-hydroxypalmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
omega-hydroxypalmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
palmitic acid + lyso-GM1a
-
733197
Pseudomonas sp.
? + H2O
-
-
-
r
palmitic acid + lyso-GM1a
-
733197
Shewanella algae
? + H2O
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Pseudomonas sp.
37
-
assay at
Shewanella algae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at, forward direction
Pseudomonas sp.
6
-
hydrolytic activity optimum
Shewanella algae
7.5
-
assay at, reverse direction
Pseudomonas sp.
7.5
-
synthetic activity optimum
Shewanella algae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
9
activity range, hydrolysis
Shewanella algae
5.5
10
activity range, synthesis
Shewanella algae
Other publictions for EC 3.5.1.69
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733197
Han
Comprehensive characterization ...
Pseudomonas sp., Shewanella algae, Shewanella algae G8
Appl. Microbiol. Biotechnol.
99
6715-6726
2015
1
2
1
-
-
-
13
-
-
7
-
-
-
9
-
-
1
-
-
1
-
-
53
-
6
2
-
-
-
4
2
-
-
-
-
-
1
2
1
-
-
-
-
-
13
-
-
-
7
-
-
-
-
-
1
-
1
-
-
53
-
2
-
-
-
4
2
-
-
-
-
-
-
-
-
754363
Huang
A facile method for controlli ...
Shewanella algae, Shewanella algae G8
J. Lipid Res.
56
1836-1842
2015
4
1
1
-
-
-
-
-
-
4
-
-
-
4
-
-
1
-
-
-
-
-
11
-
2
1
-
-
-
1
-
-
-
-
-
-
4
1
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
11
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
720087
Frank
-
Microscale analysis of glycosp ...
Pseudomonas sp.
J. Carbohydr. Chem.
30
233-248
2011
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712680
Li
Mass spectrometry of fluorocar ...
Pseudomonas sp. TK4
J. Mass Spectrom.
45
504-519
2010
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713488
Kuchar
Semisynthesis of C17:0 isoform ...
Pseudomonas sp. TK4
Rapid Commun. Mass Spectrom.
24
2393-2399
2010
-
2
-
-
-
1
-
-
-
-
-
-
-
4
-
-
-
1
-
1
-
1
1
-
2
1
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
652249
Furusato
Molecular cloning and characte ...
Shewanella algae, Shewanella algae G8
J. Biol. Chem.
277
17300-17307
2002
2
-
1
-
-
-
1
-
-
3
2
-
-
4
-
1
1
-
-
-
1
-
10
1
-
-
-
-
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
-
1
-
-
-
3
2
-
-
-
1
1
-
-
1
-
10
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
288938
Ito
A novel enzyme that cleaves th ...
Pseudomonas sp.
J. Biol. Chem.
41
24370-24374
1995
-
-
-
-
-
-
5
-
-
-
1
-
-
1
-
-
1
-
-
-
-
1
12
1
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
1
-
-
-
-
1
-
-
-
1
12
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
171986
Hirabayashi
A novel glycosphingolipid hydr ...
Nocardia sp.
J. Biochem.
103
1-4
1988
1
-
-
-
-
-
6
-
1
2
-
-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
2
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
6
-
-
1
2
-
-
-
-
-
-
-
-
-
-
8
-
-
-
2
-
1
-
1
-
-
-
-
-
-
-