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Literature summary for 3.5.1.69 extracted from

  • Furusato, M.; Sueyoshi, N.; Mitsutake, S.; Sakaguchi, K.; Kita, K.; Okino, N.; Ichinose, S.; Omori, A.; Ito, M.
    Molecular cloning and characterization of sphingolipid ceramide N-deacylase from a marine bacterium, Shewanella alga G8 (2002), J. Biol. Chem., 277, 17300-17307.
    View publication on PubMed
Search for more literature for 3.5.1.69

Activating Compound

Activating Compound Comment Organism Structure
taurodeoxycholate may substitute for Triton X-100 at 0.5% Shewanella algae
Triton X-100 required, 0.1% Shewanella algae

Cloned(Commentary)

Cloned (Comment) Organism
-
 Shewanella algae

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, strong Shewanella algae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Shewanella algae
Mg2+ activation Shewanella algae
Mn2+ activation Shewanella algae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 1 * 109800, deduced from gene sequence, 1 * 75000, SDS-PAGE Shewanella algae
109800
-
 1 * 109800, deduced from gene sequence, 1 * 75000, SDS-PAGE Shewanella algae

Organism

Organism UniProt Comment Textmining
Shewanella algae Q8RTY6
-
-
Shewanella algae G8 Q8RTY6
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification C-terminal processing of enzyme is necessary for full activity Shewanella algae

Purification (Commentary)

Purification (Comment) Organism
-
 Shewanella algae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30.4
-
 pH 6.0, 37°C Shewanella algae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
asialoganglioside GM1 + H2O
-
 Shewanella algae fatty acid + ?
-
 ?
asialoganglioside GM1 + H2O
-
 Shewanella algae G8 fatty acid + ?
-
 ?
ganglioside GD1b + H2O
-
 Shewanella algae fatty acid + ?
-
 ?
ganglioside GD1b + H2O
-
 Shewanella algae G8 fatty acid + ?
-
 ?
ganglioside GM1a + H2O
-
 Shewanella algae fatty acid + ?
-
 ?
ganglioside GM1a + H2O
-
 Shewanella algae G8 fatty acid + ?
-
 ?
ganglioside GM3 + H2O
-
 Shewanella algae fatty acid + ?
-
 ?
ganglioside GM3 + H2O
-
 Shewanella algae G8 fatty acid + ?
-
 ?
ganglioside GT1b + H2O
-
 Shewanella algae fatty acid + ?
-
 ?
ganglioside GT1b + H2O
-
 Shewanella algae G8 fatty acid + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 109800, deduced from gene sequence, 1 * 75000, SDS-PAGE Shewanella algae

pH Range

pH Minimum pH Maximum Comment Organism
5.5 6.5
-
 Shewanella algae