Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Lubrol Px | enzyme solubilized, activation | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | no or slight inhibition | Rattus norvegicus | |
ATP | 0.1 mM: 78% inhibition, 1 mM: 97% inhibition; strong competitive inhibition | Rattus norvegicus | |
citrate | 0.1 mM: 77% inhibition, 1 mM: 98% inhibition | Rattus norvegicus | |
EDTA | inhibition completely reversed by Mn2+, activity 30% restored by Ca2+ and 13% restored by Mg2+ | Rattus norvegicus | |
EGTA | - |
Rattus norvegicus | |
glutamate | 0.1 mM: 34% inhibition, 1 mM: 81% inhibition | Rattus norvegicus | |
GMP | no or slight inhibition | Rattus norvegicus | |
GTP | 0.1 mM: 90% inhibition, 1 mM: 99% inhibition; strong competitive inhibition | Rattus norvegicus | |
L-Quisqualate | 0.1 mM: 80% inhibition, 1 mM: 96% inhibition; non-competitive potent inhibitor | Rattus norvegicus | |
additional information | no or very slight inhibition by kinate, N-methyl-D-aspartate | Rattus norvegicus | |
nucleotides | strong inhibition | Rattus norvegicus | |
phosphate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
beta-Citryl-L-glutamate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | localized in particulate fractions | Rattus norvegicus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for full activity, can replace Mn2+ to some extent | Rattus norvegicus | |
Divalent metal ions | required | Rattus norvegicus | |
Fe2+ | no activation | Rattus norvegicus | |
Mg2+ | required for full activity, can replace Mn2+ to some extent | Rattus norvegicus | |
Mn2+ | required for full activity | Rattus norvegicus | |
Mn2+ | maximal activity at 0.5-1 mM | Rattus norvegicus | |
additional information | enzyme is a metalloenzyme | Rattus norvegicus | |
additional information | no activation by Ni2+, Zn2+ | Rattus norvegicus | |
additional information | no activation by Cu2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
350000 | - |
PAGE, after digestion with N-glycosidase F | Rattus norvegicus |
380000 | - |
gel filtration | Rattus norvegicus |
420000 | - |
PAGE, before digestion with N-glycosidase F | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Wistar | - |
Rattus norvegicus Wistar | - |
Wistar | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
solubilized by 0.5% Lubrol PX, most effective detergent | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0192 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-citryl-L-glutamate + H2O | deacylation of beta-citryl-L-glutamate | Rattus norvegicus | citrate + L-glutamate | - |
ir | |
beta-citryl-L-glutamate + H2O | best substrate, high substrate specificity for beta-citryl-L-glutamate | Rattus norvegicus | citrate + L-glutamate | - |
ir | |
beta-citryl-L-glutamine + H2O | at about 1/30 of that for beta-citryl-L-glutamate | Rattus norvegicus | citrate + L-glutamine | - |
ir | |
additional information | substrate specificity | Rattus norvegicus | ? | - |
? | |
additional information | little or no activity with N-formyl-L-aspartate, N-formyl-L-glutamine, N-acetyl-L-glutamate, alpha-citryl-L-glutamate, alpha- and beta-citryl-alpha-L-glutamylglutamate | Rattus norvegicus | ? | - |
? | |
additional information | no activity with N-acetyl-alpha-aspartylglutamate | Rattus norvegicus | ? | - |
? | |
additional information | little or no activity with N-acetyl-L-glutamate | Rattus norvegicus | ? | - |
? | |
additional information | substrate specificity | Rattus norvegicus Wistar | ? | - |
? | |
additional information | little or no activity with N-formyl-L-aspartate, N-formyl-L-glutamine, N-acetyl-L-glutamate, alpha-citryl-L-glutamate, alpha- and beta-citryl-alpha-L-glutamylglutamate | Rattus norvegicus Wistar | ? | - |
? | |
additional information | no activity with N-acetyl-alpha-aspartylglutamate | Rattus norvegicus Wistar | ? | - |
? | |
additional information | little or no activity with N-acetyl-L-glutamate | Rattus norvegicus Wistar | ? | - |
? | |
N-acetyl-L-aspartate + H2O | poor substrate | Rattus norvegicus | acetate + L-aspartate | - |
? | |
N-formyl-L-glutamate + H2O | poor substrate | Rattus norvegicus | formate + L-glutamate | - |
? | |
N-formyl-L-glutamate + H2O | poor substrate | Rattus norvegicus Wistar | formate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | composed of 90000, 100000, 115000 and 130000 Da subunits, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI of about pH 4.3 | Rattus norvegicus |
6.5 | 7 | in the presence of Mn2+ | Rattus norvegicus |