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Literature summary for 3.5.1.67 extracted from

  • Powell, G.K.; Dekker, E.E.
    Purification and properties of a 4-methylene-L-glutamine amidohydrolase from peanut leaves (1983), J. Biol. Chem., 258, 8677-8683.
    View publication on PubMed
Search for more literature for 3.5.1.67

Activating Compound

Activating Compound Comment Organism Structure
additional information activation studies Arachis hypogaea
additional information no activation by thiols or sulfhydryl-reacting reagents Arachis hypogaea
SDS activation Arachis hypogaea
SDS 0.001-1%, 2-3fold stimulation, 4-methylene-L-glutamine or glutamine as substrate Arachis hypogaea

Inhibitors

Inhibitors Comment Organism Structure
arsenate no inhibition Arachis hypogaea
BO33- strong inhibition, 1 mM: 50% inhibition Arachis hypogaea
Cu2+ no inhibition Arachis hypogaea
additional information inhibition studies; no inhibition by thiols, sulfhydryl-reacting reagents, glutaramic acid, glutarimide, acrylamide, methacrylamide, succinamic acid, acetamide, N-benzoyl-4-methylene-L-glutamine, isoglutamine, 4-methyleneisoglutamine, N-ethylmaleimide, p-mercuribenzoate, 5,5'-dithiobis(2-nitrobenzoic acid), dithiothreitol, chelating agents, EDTA, EGTA, 8-hydroxyquinoline, 8-hydroxyquinoline sulfonic acid, citrate, 2,2'-dipyridyl, 1 mM of following metal ions: Ca2+, Cd2+, Cu2+, Fe2+, Fe3+, Hg2+, Mg2+, Mn2+, Ni2+, Zn2+, 0.1-10 mM: CO32-, SO32-, SO42-, NO3-, AsO43- Arachis hypogaea
Urea slight inhibition, 10 mM: 10% inhibition Arachis hypogaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data Arachis hypogaea
3
-
 4-methylene-L-glutamine
-
 Arachis hypogaea
19.5
-
 L-glutamine
-
 Arachis hypogaea

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol present in soluble fraction Arachis hypogaea 5829
-
additional information subcellular localization Arachis hypogaea
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal ion not required Arachis hypogaea
phosphate no activation observed Arachis hypogaea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 gel filtration Arachis hypogaea
48600
-
 1 * 48600, SDS-PAGE Arachis hypogaea
73000
-
 sucrose density gradient centrifugation, possibly the result of aggregation in the presence of high sucrose concentrations Arachis hypogaea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-methylene-L-glutamine + H2O Arachis hypogaea highly specific ?
-
 ?
4-methylene-L-glutamine + H2O Arachis hypogaea implicated in the process of N-translocation ?
-
 ?
4-methylene-L-glutamine + H2O Arachis hypogaea initial enzyme for catalyzing 4-methylene-L-glutamine degradation ?
-
 ?
4-methylene-L-glutamine + H2O Arachis hypogaea implicated in the transport of nitrogen to the developing shoots and leaves of germinating peanuts, involved in releasing nitrogen to the plants ?
-
 ?
additional information Arachis hypogaea implicated in the process of N-translocation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
 multiple forms: AH-1, AH-2, AH-3
-
Arachis hypogaea
-
 multiple electrophoretically distinct forms
-
Arachis hypogaea
-
 Valencia peanut
-
Arachis hypogaea
-
 peanut, groundnut
-

Purification (Commentary)

Purification (Comment) Organism
from fresh or frozen leaves Arachis hypogaea
multiple electrophoretically distinct forms Arachis hypogaea
partial Arachis hypogaea

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon 27% of total activity Arachis hypogaea
-
hypocotyl 2% of total activity Arachis hypogaea
-
leaf 14- to 16-day germinated peanuts Arachis hypogaea
-
leaf 68% of total activity Arachis hypogaea
-
additional information tissue distribution, not in root, imbibed seeds and mature plants Arachis hypogaea
-
additional information level of 4-methylene-L-glutamine amidohydrolase activity increases rapidly with germination to a maximum after 3 weeks Arachis hypogaea
-
stem 3% of total activity Arachis hypogaea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
49.3 106 3 multiple forms of 4-methylene-L-glutamine amidohydrolase: AH-1: 49.3 micromol/min/mg, AH-2: 106.0 micromol/min/mg, AH-3: 77.3 micromol/min/mg Arachis hypogaea

Storage Stability

Storage Stability Organism
4°C, stable for at least 4 months Arachis hypogaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methyl-L-glutamine + H2O about 9% of the rate of 4-methylene-L-glutamine hydrolysis Arachis hypogaea 4-methyl-L-glutamate + NH3
-
 ?
4-methylene-L-glutamine + H2O highly specific Arachis hypogaea 4-methylene-L-glutamate + NH3
-
 ?
4-methylene-L-glutamine + H2O highly specific Arachis hypogaea ?
-
 ?
4-methylene-L-glutamine + H2O implicated in the process of N-translocation Arachis hypogaea ?
-
 ?
4-methylene-L-glutamine + H2O initial enzyme for catalyzing 4-methylene-L-glutamine degradation Arachis hypogaea ?
-
 ?
4-methylene-L-glutamine + H2O implicated in the transport of nitrogen to the developing shoots and leaves of germinating peanuts, involved in releasing nitrogen to the plants Arachis hypogaea ?
-
 ?
L-glutamine + H2O about 20% of the rate of 4-methylene-L-glutamine hydrolysis Arachis hypogaea L-glutamate + NH3
-
 ?
additional information substrate specificity Arachis hypogaea ?
-
 ?
additional information 5-carbon amino acid amide structure is essential for activity Arachis hypogaea ?
-
 ?
additional information L-asparagine: not a substrate Arachis hypogaea ?
-
 ?
additional information glutaramic acid, glutarimide, acrylamide, methacrylamide, succinamic acid, acetamide, urea, N-benzoyl-4-methylene-L-glutamine, isoglutamine, 4-methyleneisoglutamine: no substrates Arachis hypogaea ?
-
 ?
additional information implicated in the process of N-translocation Arachis hypogaea ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 48600, SDS-PAGE Arachis hypogaea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Arachis hypogaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9 broad pH optimum Arachis hypogaea

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10.5 pH 6.5: 47% of maximal activity, pH 10.5: 27% of maximal activity Arachis hypogaea