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Literature summary for 3.5.1.67 extracted from

  • Winter, H.C.; Dekker, E.E.
    4-Methyleneglutamine amidohydrolase from peanut leaves. Preparation, catalytic properties, and immunological responses of a highly purified form of the enzyme (1991), Plant Physiol., 95, 206-212.
    View publication on PubMed
Search for more literature for 3.5.1.67

Activating Compound

Activating Compound Comment Organism Structure
ADP slight activation Arachis hypogaea
benzalkonium chloride activation, 126% activity Arachis hypogaea
bovine serum albumin activation, 0.5%: 180% activity Arachis hypogaea
Detergents ionic or non-ionic detergents, 3-4fold activation Arachis hypogaea
methionine sulfoximine very slight activation Arachis hypogaea
additional information activation studies Arachis hypogaea
additional information no activation by dithiothreitol Arachis hypogaea
rabbit preimmune serum activation, 0.5%: 180% activity Arachis hypogaea
SDS activation Arachis hypogaea
SDS 0.001-1%, 2-3fold stimulation, 4-methylene-L-glutamine or glutamine as substrate Arachis hypogaea
sodium deoxycholate activation, 160% activity Arachis hypogaea
sodium octanoate activation, 130% activity Arachis hypogaea
Triton X-100 activation, 180% activity Arachis hypogaea

Inhibitors

Inhibitors Comment Organism Structure
4-methylene-L-glutamate slight inhibition Arachis hypogaea
4-methylene-L-glutamine competitive, glutamine as substrate, Ki: 30 mM Arachis hypogaea
albizziine 3-ureidoalanine, glutamine analog, competitive, 4 mM: 33% inhibition, 10 mM: 50% inhibition, 20 mM: 68% inhibition Arachis hypogaea
BO33- strong inhibition, 1 mM: 55% inhibition, 2 mM: 70% inhibition, 5 mM: 87% inhibition Arachis hypogaea
diazo-oxo-norleucine very slight inhibition, 4 mM: 7% inhibition Arachis hypogaea
L-glutamine competitive, 20 mM: 50% inhibition, 4-methylene-L-glutamine as substrate Arachis hypogaea
additional information inhibition studies; no inhibition by methionine sulfoximine, ADP Arachis hypogaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data Arachis hypogaea
5
-
 4-methylene-L-glutamine
-
 Arachis hypogaea
20
-
 L-glutamine
-
 Arachis hypogaea

Metals/Ions

Metals/Ions Comment Organism Structure
phosphate slight activation Arachis hypogaea
Phosphate esters slight activation Arachis hypogaea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-methylene-L-glutamine + H2O Arachis hypogaea implicated in the process of N-translocation ?
-
 ?
4-methylene-L-glutamine + H2O Arachis hypogaea key evidence in establishing N-translocating role for this nonprotein amino acid ?
-
 ?
4-methylene-L-glutamine + H2O Arachis hypogaea highly specific ?
-
 ?
additional information Arachis hypogaea implicated in the process of N-translocation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
 Valencia, cv Tennessee Red
-
Arachis hypogaea
-
 two electrophoretically distinct, immunologically identical forms with similar catalytic properties
-
Arachis hypogaea
-
 multiple electrophoretically distinct forms
-
Arachis hypogaea
-
 peanut, groundnut
-

Purification (Commentary)

Purification (Comment) Organism
multiple electrophoretically distinct forms Arachis hypogaea
two catalytically active forms Arachis hypogaea

Source Tissue

Source Tissue Comment Organism Textmining
leaf 14- to 16-day germinated peanuts Arachis hypogaea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
349
-
-
 Arachis hypogaea

Storage Stability

Storage Stability Organism
4°C, in solution, stable for many months Arachis hypogaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylene-L-glutamine + H2O highly specific Arachis hypogaea 4-methylene-L-glutamate + NH3
-
 ?
4-methylene-L-glutamine + H2O implicated in the process of N-translocation Arachis hypogaea ?
-
 ?
4-methylene-L-glutamine + H2O key evidence in establishing N-translocating role for this nonprotein amino acid Arachis hypogaea ?
-
 ?
4-methylene-L-glutamine + H2O highly specific Arachis hypogaea ?
-
 ?
L-glutamine + H2O
-
 Arachis hypogaea L-glutamate + NH3
-
 ?
additional information implicated in the process of N-translocation Arachis hypogaea ?
-
 ?

Subunits

Subunits Comment Organism
additional information two electrophoretically distinct catalytically active forms: band I: 57000 and 50000 Arachis hypogaea
additional information probably a heterodimer, band II: 50000, probably a homodimer, PAGE Arachis hypogaea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Arachis hypogaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 AH-3: pI: 5.1 Arachis hypogaea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
30
-
 4-methylene-L-glutamine competitive, glutamine as substrate Arachis hypogaea