A novel enzyme, Nalpha-benzyloxycarbonyl amino acid urethane hydrolase IV
Matsumura, E.; Shin, T.; Murao, S.; Sakaguchi, M.; Kawano, T.; Agric. Biol. Chem. 49, 3643-3645 (1985)
No PubMed abstract available
Data extracted from this reference:
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
1 mM: complete inhibition
Soil bacterium
iodoacetate
1 mM: strong inhibition
Soil bacterium
p-chloromercuribenzoate
1 mM: strong inhibition
Soil bacterium
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
required for activity, optimum concentration about 1 mM
Soil bacterium
divalent cations
required for activity
Soil bacterium
Zn2+
required for activity, optimum concentration about 1 mM
Soil bacterium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
strain F-96, x * 39000, SDS-PAGE
Soil bacterium
116000
-
strain F-96, gel filtration
Soil bacterium
Organism
Organism
UniProt
Commentary
Textmining
Soil bacterium
-
-
-
Soil bacterium F-96
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
strain F-96
Soil bacterium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
substrate specificity
172071
Soil bacterium
?
-
-
-
?
additional information
hydrolyzes urethane bonds in Nalpha-benzyloxycarbonyl amino acids
172071
Soil bacterium
?
-
-
-
?
additional information
broadly selective amino acid specificity
172071
Soil bacterium
?
-
-
-
?
additional information
specific for L-isomers
172071
Soil bacterium
?
-
-
-
?
additional information
no activity with Nalpha-benzyloxycarbonyl-Trp, Nalpha-benzyloxycarbonyl-Pro, Nalpha-benzyloxycarbonyl-Arg, Nalpha-benzyloxycarbonyl-Lys, Nalpha-benzyloxycarbonyl-beta-Ala, Nalpha-benzyloxycarbonyl-D-Asp, Nalpha-benzyloxycarbonyl-D-Glu, Nalpha-benzyloxycarbonyl-D-Gln, Nalpha-benzyloxycarbonyl-Leu-NH2, Nalpha-tert-butoxycarbonyl-Trp, Nalpha-tert-butoxycarbonyl-Pro, Nalpha-tert-butoxycarbonyl-Arg, Nalpha-tert-butoxycarbonyl-Lys, Nalpha-tert-butoxycarbonyl-beta-Ala, Nalpha-tert-butoxycarbonyl-D-Leu, Nalpha-tert-butoxycarbonyl-D-Met, Nalpha-acetyl-Pro, Nalpha-acetyl-Arg, Nalpha-acetyl-Lys, Nalpha-methyl-Asp, Nalpha-amidino-Asp, Nalpha-tosyl-Glu, folic acid, methotrexate or casein
172071
Soil bacterium
?
-
-
-
?
additional information
inactive toward Nalpha-benzyloxycarbonyl-D-amino acids
172071
Soil bacterium
?
-
-
-
?
additional information
requires substrates with free carboxyl group of amino acids
172071
Soil bacterium
?
-
-
-
?
additional information
substrate specificity
172071
Soil bacterium F-96
?
-
-
-
?
additional information
hydrolyzes urethane bonds in Nalpha-benzyloxycarbonyl amino acids
172071
Soil bacterium F-96
?
-
-
-
?
additional information
broadly selective amino acid specificity
172071
Soil bacterium F-96
?
-
-
-
?
additional information
specific for L-isomers
172071
Soil bacterium F-96
?
-
-
-
?
additional information
no activity with Nalpha-benzyloxycarbonyl-Trp, Nalpha-benzyloxycarbonyl-Pro, Nalpha-benzyloxycarbonyl-Arg, Nalpha-benzyloxycarbonyl-Lys, Nalpha-benzyloxycarbonyl-beta-Ala, Nalpha-benzyloxycarbonyl-D-Asp, Nalpha-benzyloxycarbonyl-D-Glu, Nalpha-benzyloxycarbonyl-D-Gln, Nalpha-benzyloxycarbonyl-Leu-NH2, Nalpha-tert-butoxycarbonyl-Trp, Nalpha-tert-butoxycarbonyl-Pro, Nalpha-tert-butoxycarbonyl-Arg, Nalpha-tert-butoxycarbonyl-Lys, Nalpha-tert-butoxycarbonyl-beta-Ala, Nalpha-tert-butoxycarbonyl-D-Leu, Nalpha-tert-butoxycarbonyl-D-Met, Nalpha-acetyl-Pro, Nalpha-acetyl-Arg, Nalpha-acetyl-Lys, Nalpha-methyl-Asp, Nalpha-amidino-Asp, Nalpha-tosyl-Glu, folic acid, methotrexate or casein
172071
Soil bacterium F-96
?
-
-
-
?
Nalpha-(4-aminobenzoyl)-L-Glu + H2O
poor substrate
172071
Soil bacterium
1-amino-4-hydroxybenzene + CO2 + L-Glu
-
-
-
?
Nalpha-(4-hydroxybenzoyl)-L-Asp + H2O
poor substrate
172071
Soil bacterium
1,4-dihydroxybenzene + CO2 + L-Asp
-
-
-
?
Nalpha-acetyl-L-Asp + H2O
poor substrate
172071
Soil bacterium
methanol + CO2 + L-Asp
-
-
-
?
Nalpha-acetyl-L-Glu + H2O
poor substrate
172071
Soil bacterium
methanol + CO2 + L-Glu
-
-
-
?
Nalpha-acetyl-L-Leu + H2O
40% of the rate of Nalpha-benzyloxycarbonyl-Leu hydrolysis
172071
Soil bacterium
methanol + CO2 + L-Leu
-
-
-
?
Nalpha-benzoyl-L-Glu + H2O
-
172071
Soil bacterium
phenol + CO2 + L-Glu
-
-
-
?
Nalpha-benzoyl-L-Leu + H2O
-
172071
Soil bacterium
phenol + CO2 + L-Leu
-
-
-
?
Nalpha-benzyloxycarbonyl-Gly + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + Gly
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Ala + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Ala
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Asn + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Asn
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Asp + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Asp
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Cys + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Cys
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Gln + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Gln
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Glu + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Glu
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-His + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-His
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Ile + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Ile
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Met + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Met
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Phe + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Phe
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Ser + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Ser
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Thr + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Thr
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Tyr + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Tyr
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Val + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Val
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-Leu + H2O
best substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Leu
-
172071
Soil bacterium
?
Nalpha-tert-butoxycarbonyl-Gly + H2O
-
172071
Soil bacterium
tert-butanol + CO2 + Gly
-
-
-
?
Nalpha-tert-butoxycarbonyl-L-Ala + H2O
-
172071
Soil bacterium
tert-butanol + CO2 + L-Ala
-
-
-
?
Nalpha-tert-butoxycarbonyl-L-Asp + H2O
-
172071
Soil bacterium
tert-butanol + CO2 + L-Asp
-
-
-
?
Nalpha-tert-butoxycarbonyl-L-Gln + H2O
poor substrate
172071
Soil bacterium
tert-butanol + CO2 + L-Gln
-
-
-
?
Nalpha-tert-butoxycarbonyl-L-Leu + H2O
80% of the rate of Nalpha-benzyloxycarbonyl-Leu hydrolysis
172071
Soil bacterium
tert-butanol + CO2 + L-Leu
-
-
-
?
Nalpha-tert-butoxycarbonyl-L-Met + H2O
poor substrate
172071
Soil bacterium
tert-butanol + CO2 + L-Met
-
-
-
?
Nalpha-tert-butoxycarbonyl-L-Val + H2O
poor substrate
172071
Soil bacterium
tert-butanol + CO2 + L-Val
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
strain F-96, x * 39000, SDS-PAGE
Soil bacterium
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Soil bacterium
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
17 h, pH 6-7, stable at
Soil bacterium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 3.31
Soil bacterium
5.1
-
-
Soil bacterium
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
7
17 h, 37°C, stable at
Soil bacterium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
1 mM: complete inhibition
Soil bacterium
iodoacetate
1 mM: strong inhibition
Soil bacterium
p-chloromercuribenzoate
1 mM: strong inhibition
Soil bacterium
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
required for activity, optimum concentration about 1 mM
Soil bacterium
divalent cations
required for activity
Soil bacterium
Zn2+
required for activity, optimum concentration about 1 mM
Soil bacterium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
strain F-96, x * 39000, SDS-PAGE
Soil bacterium
116000
-
strain F-96, gel filtration
Soil bacterium
Purification (Commentary) (protein specific)
Commentary
Organism
strain F-96
Soil bacterium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
substrate specificity
172071
Soil bacterium
?
-
-
-
?
additional information
hydrolyzes urethane bonds in Nalpha-benzyloxycarbonyl amino acids
172071
Soil bacterium
?
-
-
-
?
additional information
broadly selective amino acid specificity
172071
Soil bacterium
?
-
-
-
?
additional information
specific for L-isomers
172071
Soil bacterium
?
-
-
-
?
additional information
no activity with Nalpha-benzyloxycarbonyl-Trp, Nalpha-benzyloxycarbonyl-Pro, Nalpha-benzyloxycarbonyl-Arg, Nalpha-benzyloxycarbonyl-Lys, Nalpha-benzyloxycarbonyl-beta-Ala, Nalpha-benzyloxycarbonyl-D-Asp, Nalpha-benzyloxycarbonyl-D-Glu, Nalpha-benzyloxycarbonyl-D-Gln, Nalpha-benzyloxycarbonyl-Leu-NH2, Nalpha-tert-butoxycarbonyl-Trp, Nalpha-tert-butoxycarbonyl-Pro, Nalpha-tert-butoxycarbonyl-Arg, Nalpha-tert-butoxycarbonyl-Lys, Nalpha-tert-butoxycarbonyl-beta-Ala, Nalpha-tert-butoxycarbonyl-D-Leu, Nalpha-tert-butoxycarbonyl-D-Met, Nalpha-acetyl-Pro, Nalpha-acetyl-Arg, Nalpha-acetyl-Lys, Nalpha-methyl-Asp, Nalpha-amidino-Asp, Nalpha-tosyl-Glu, folic acid, methotrexate or casein
172071
Soil bacterium
?
-
-
-
?
additional information
inactive toward Nalpha-benzyloxycarbonyl-D-amino acids
172071
Soil bacterium
?
-
-
-
?
additional information
requires substrates with free carboxyl group of amino acids
172071
Soil bacterium
?
-
-
-
?
additional information
substrate specificity
172071
Soil bacterium F-96
?
-
-
-
?
additional information
hydrolyzes urethane bonds in Nalpha-benzyloxycarbonyl amino acids
172071
Soil bacterium F-96
?
-
-
-
?
additional information
broadly selective amino acid specificity
172071
Soil bacterium F-96
?
-
-
-
?
additional information
specific for L-isomers
172071
Soil bacterium F-96
?
-
-
-
?
additional information
no activity with Nalpha-benzyloxycarbonyl-Trp, Nalpha-benzyloxycarbonyl-Pro, Nalpha-benzyloxycarbonyl-Arg, Nalpha-benzyloxycarbonyl-Lys, Nalpha-benzyloxycarbonyl-beta-Ala, Nalpha-benzyloxycarbonyl-D-Asp, Nalpha-benzyloxycarbonyl-D-Glu, Nalpha-benzyloxycarbonyl-D-Gln, Nalpha-benzyloxycarbonyl-Leu-NH2, Nalpha-tert-butoxycarbonyl-Trp, Nalpha-tert-butoxycarbonyl-Pro, Nalpha-tert-butoxycarbonyl-Arg, Nalpha-tert-butoxycarbonyl-Lys, Nalpha-tert-butoxycarbonyl-beta-Ala, Nalpha-tert-butoxycarbonyl-D-Leu, Nalpha-tert-butoxycarbonyl-D-Met, Nalpha-acetyl-Pro, Nalpha-acetyl-Arg, Nalpha-acetyl-Lys, Nalpha-methyl-Asp, Nalpha-amidino-Asp, Nalpha-tosyl-Glu, folic acid, methotrexate or casein
172071
Soil bacterium F-96
?
-
-
-
?
Nalpha-(4-aminobenzoyl)-L-Glu + H2O
poor substrate
172071
Soil bacterium
1-amino-4-hydroxybenzene + CO2 + L-Glu
-
-
-
?
Nalpha-(4-hydroxybenzoyl)-L-Asp + H2O
poor substrate
172071
Soil bacterium
1,4-dihydroxybenzene + CO2 + L-Asp
-
-
-
?
Nalpha-acetyl-L-Asp + H2O
poor substrate
172071
Soil bacterium
methanol + CO2 + L-Asp
-
-
-
?
Nalpha-acetyl-L-Glu + H2O
poor substrate
172071
Soil bacterium
methanol + CO2 + L-Glu
-
-
-
?
Nalpha-acetyl-L-Leu + H2O
40% of the rate of Nalpha-benzyloxycarbonyl-Leu hydrolysis
172071
Soil bacterium
methanol + CO2 + L-Leu
-
-
-
?
Nalpha-benzoyl-L-Glu + H2O
-
172071
Soil bacterium
phenol + CO2 + L-Glu
-
-
-
?
Nalpha-benzoyl-L-Leu + H2O
-
172071
Soil bacterium
phenol + CO2 + L-Leu
-
-
-
?
Nalpha-benzyloxycarbonyl-Gly + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + Gly
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Ala + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Ala
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Asn + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Asn
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Asp + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Asp
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Cys + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Cys
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Gln + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Gln
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Glu + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Glu
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-His + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-His
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Ile + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Ile
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Met + H2O
-
172071
Soil bacterium
benzyl alcohol + CO2 + L-Met
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Phe + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Phe
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Ser + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Ser
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Thr + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Thr
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Tyr + H2O
very poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Tyr
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-L-Val + H2O
poor substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Val
-
172071
Soil bacterium
?
Nalpha-benzyloxycarbonyl-Leu + H2O
best substrate
172071
Soil bacterium
benzyl alcohol + CO2 + L-Leu
-
172071
Soil bacterium
?
Nalpha-tert-butoxycarbonyl-Gly + H2O
-
172071
Soil bacterium
tert-butanol + CO2 + Gly
-
-
-
?
Nalpha-tert-butoxycarbonyl-L-Ala + H2O
-
172071
Soil bacterium
tert-butanol + CO2 + L-Ala
-
-
-
?
Nalpha-tert-butoxycarbonyl-L-Asp + H2O
-
172071
Soil bacterium
tert-butanol + CO2 + L-Asp
-
-
-
?
Nalpha-tert-butoxycarbonyl-L-Gln + H2O
poor substrate
172071
Soil bacterium
tert-butanol + CO2 + L-Gln
-
-
-
?
Nalpha-tert-butoxycarbonyl-L-Leu + H2O
80% of the rate of Nalpha-benzyloxycarbonyl-Leu hydrolysis
172071
Soil bacterium
tert-butanol + CO2 + L-Leu
-
-
-
?
Nalpha-tert-butoxycarbonyl-L-Met + H2O
poor substrate
172071
Soil bacterium
tert-butanol + CO2 + L-Met
-
-
-
?
Nalpha-tert-butoxycarbonyl-L-Val + H2O
poor substrate
172071
Soil bacterium
tert-butanol + CO2 + L-Val
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
strain F-96, x * 39000, SDS-PAGE
Soil bacterium
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Soil bacterium
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
17 h, pH 6-7, stable at
Soil bacterium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
additional information
-
pI: 3.31
Soil bacterium
5.1
-
-
Soil bacterium
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
7
17 h, 37°C, stable at
Soil bacterium
Other publictions for EC 3.5.1.64
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
172071
Matsumura
-
A novel enzyme, Nalpha-benzylo ...
Soil bacterium, Soil bacterium F-96
Agric. Biol. Chem.
49
3643-3645
1985
-
-
-
-
-
-
3
-
-
3
2
-
-
2
-
-
1
-
-
-
-
-
42
1
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
3
2
-
-
-
-
1
-
-
-
-
42
1
1
-
1
-
2
-
1
-
-
-
-
-
-
-