Literature summary for 3.5.1.60 extracted from

Sakura, Y.; Tsuboi, K.; Uyama, T.; Zhang, X.; Taoka, R.; Sugimoto, M.; Kakehi, Y.; Ueda, N.
A quantitative study on splice variants of N-acylethanolamine acid amidase in human prostate cancer cells and other cells (2016), Biochim. Biophys. Acta, 1861, 1951-1958 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene NAAA, genotyping, six splice variants (a1, b1, c1, a2, b2, and c2), whereof four major splice variants (a1, a2, b2, and c2) are identified in a human prostate cancer cell line LNCaP, they are composed of exons 1-11, exons 1-10 and 12, exons 1-9 and 12, and exons 1-8 and 12, respectively, quantitative PCR expression analysis, method development for individual quantification. Variants a1 and a2 encode the same full-length NAAA protein, which is catalytically active, while b2 and c2 are translated to C-terminally truncated proteins. Recombinant expression in HEK-293 cells. The truncated forms are detected as catalytically inactive precursor proteins, but not as mature forms, expression of N-terminally FLAG-tagged isoform A of human NAAA, corresponding to splice variant a1, in HEK-293 cells. The mature enzyme form loses the N-terminal FLAG tag	Homo sapiens

Cloned (Comment)

Organism

gene NAAA, genotyping, six splice variants (a1, b1, c1, a2, b2, and c2), whereof four major splice variants (a1, a2, b2, and c2) are identified in a human prostate cancer cell line LNCaP, they are composed of exons 1-11, exons 1-10 and 12, exons 1-9 and 12, and exons 1-8 and 12, respectively, quantitative PCR expression analysis, method development for individual quantification. Variants a1 and a2 encode the same full-length NAAA protein, which is catalytically active, while b2 and c2 are translated to C-terminally truncated proteins. Recombinant expression in HEK-293 cells. The truncated forms are detected as catalytically inactive precursor proteins, but not as mature forms, expression of N-terminally FLAG-tagged isoform A of human NAAA, corresponding to splice variant a1, in HEK-293 cells. The mature enzyme form loses the N-terminal FLAG tag

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
L325A	site-directed mutagenesis of isozyme A, the mutant shows reduced activity compared to wild-type	Homo sapiens
L325T	site-directed mutagenesis of isozyme A, the mutant shows reduced activity compared to wild-type	Homo sapiens
T335A	site-directed mutagenesis of isozyme A, the mutant is almost inactive	Homo sapiens
T335V	site-directed mutagenesis of isozyme A, the mutant is almost inactive	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
lysosome	-	Homo sapiens	5764	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
palmitoylethanolamide + H2O	Homo sapiens	-	palmitate + ethanolamine	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q02083	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
CMK cell	-	Homo sapiens	-
DU-145 cell	-	Homo sapiens	-
HEK-293 cell	-	Homo sapiens	-
HeLa cell	low expression level	Homo sapiens	-
LNCaP cell	high expression level	Homo sapiens	-
MCF-7 cell	-	Homo sapiens	-
additional information	all cell types examined express NAAA but its expression levels vary by cell type, expression patterns of splicing variants a1, a2, b2, and c2, overview. Splicing variants a1 and b2 show the highest and lowest expression levels, respectively, among the four variants in all of the prostate cells and other human cells	Homo sapiens	-
PC-3 cell	-	Homo sapiens	-
prostate gland	-	Homo sapiens	-
prostate gland cancer cell	the total levels of NAAA mRNAs in androgen-sensitive cells like LNCaP are higher than those in androgen-insensitive cells	Homo sapiens	-
THP-1 cell	high expression level	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
palmitoylethanolamide + H2O	-	Homo sapiens	palmitate + ethanolamine	-	?

Subunits

Subunits	Comment	Organism
?	x * 26300, recombinant untagged isozyme A, SDS-PAGE, x * 24200, isozyme B, SDS-PAGE, x * 222000, isozyme C, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
N-acylethanolamine acid amidase	-	Homo sapiens
NAAA	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
additional information	residues Leu325 and Thr335 are catalytically important for isozyme A, and the latter one is indispensable for the enzyme activity of NAAA	Homo sapiens