BRENDA - Enzyme Database show
show all sequences of 3.5.1.6

Potential application of N-carbamoyl-beta-alanine amidohydrolase from Agrobacterium tumefaciens C58 for beta-amino acid production

Martinez-Gomez, A.I.; Martinez-Rodriguez, S.; Pozo-Dengra, J.; Tessaro, D.; Servi, S.; Clemente-Jimenez, J.M.; Rodriguez-Vico, F.; Las Heras-Vazquez, F.J.; Appl. Environ. Microbiol. 75, 514-520 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Agrobacterium tumefaciens
Engineering
Amino acid exchange
Commentary
Organism
R291E
R291 is the key residue for recognition of the substrate carboxyl group
Agrobacterium tumefaciens
R291K
R291 is the key residue for recognition of the substrate carboxyl group
Agrobacterium tumefaciens
R291Q
R291 is the key residue for recognition of the substrate carboxyl group
Agrobacterium tumefaciens
Inhibitors
Inhibitors
Commentary
Organism
Structure
8-Hydroxyquinoline-5-sulfonic acid
-
Agrobacterium tumefaciens
Cu2+
strong inhibition
Agrobacterium tumefaciens
EDTA
-
Agrobacterium tumefaciens
Hg2+
slight inhibition
Agrobacterium tumefaciens
Zn2+
slight inhibition
Agrobacterium tumefaciens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
2-amino-3-ureidopropionic acid
-
Agrobacterium tumefaciens
2.14
-
3-ureidopropionic acid
-
Agrobacterium tumefaciens
3.44
-
3-methyl-3-ureidopropionic acid
-
Agrobacterium tumefaciens
6.59
-
2-methyl-3-ureidopropionic acid
-
Agrobacterium tumefaciens
10.58
-
2-ureidoethanesulfonic acid
-
Agrobacterium tumefaciens
20.15
-
2-ureidoethane phosphonic acid
-
Agrobacterium tumefaciens
84.37
-
2-phenyl-3-ureidopropionic acid
-
Agrobacterium tumefaciens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
-
Agrobacterium tumefaciens
Mn2+
-
Agrobacterium tumefaciens
Ni2+
-
Agrobacterium tumefaciens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Agrobacterium tumefaciens
90000
-
gel filtration
Agrobacterium tumefaciens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agrobacterium tumefaciens
-
-
-
Agrobacterium tumefaciens C58 / ATCC 33970
-
-
-
Purification (Commentary)
Commentary
Organism
-
Agrobacterium tumefaciens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens
3-aminoalanine + CO2 + NH3
-
-
-
?
2-amino-3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens C58 / ATCC 33970
3-aminoalanine + CO2 + NH3
-
-
-
?
2-methyl-3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens
alpha-methyl-beta-alanine + CO2 + NH3
-
-
-
?
2-methyl-3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens C58 / ATCC 33970
alpha-methyl-beta-alanine + CO2 + NH3
-
-
-
?
2-phenyl-3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens
3-amino-2-phenylpropanoic acid + CO2 + NH3
-
-
-
?
2-phenyl-3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens C58 / ATCC 33970
3-amino-2-phenylpropanoic acid + CO2 + NH3
-
-
-
?
2-ureidoethane phosphonic acid + H2O
-
695782
Agrobacterium tumefaciens
ciliatine + CO2 + NH3
-
-
-
?
2-ureidoethane phosphonic acid + H2O
-
695782
Agrobacterium tumefaciens C58 / ATCC 33970
ciliatine + CO2 + NH3
-
-
-
?
2-ureidoethanesulfonic acid + H2O
-
695782
Agrobacterium tumefaciens
taurine + CO2 + NH3
-
-
-
?
3-methyl-3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens
beta-homoalanine + CO2 + NH3
-
-
-
?
3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens
beta-alanine + CO2 + NH3
-
-
-
?
N-carbamoyl-beta-alanine + H2O
-
695782
Agrobacterium tumefaciens
beta-alanine + CO2 + NH3
-
-
-
?
N-carbamoyl-beta-alanine + H2O
-
695782
Agrobacterium tumefaciens C58 / ATCC 33970
beta-alanine + CO2 + NH3
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 45000, SDS-PAGE
Agrobacterium tumefaciens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Agrobacterium tumefaciens
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
after 60 min 20% activity
Agrobacterium tumefaciens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.13
-
2-phenyl-3-ureidopropionic acid
-
Agrobacterium tumefaciens
0.15
-
2-amino-3-ureidopropionic acid
-
Agrobacterium tumefaciens
0.2
-
3-methyl-3-ureidopropionic acid
-
Agrobacterium tumefaciens
0.23
-
2-ureidoethane phosphonic acid
-
Agrobacterium tumefaciens
5.84
-
2-ureidoethanesulfonic acid
-
Agrobacterium tumefaciens
24.39
-
2-methyl-3-ureidopropionic acid
-
Agrobacterium tumefaciens
25.71
-
3-ureidopropionic acid
-
Agrobacterium tumefaciens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Agrobacterium tumefaciens
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Agrobacterium tumefaciens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
R291E
R291 is the key residue for recognition of the substrate carboxyl group
Agrobacterium tumefaciens
R291K
R291 is the key residue for recognition of the substrate carboxyl group
Agrobacterium tumefaciens
R291Q
R291 is the key residue for recognition of the substrate carboxyl group
Agrobacterium tumefaciens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
8-Hydroxyquinoline-5-sulfonic acid
-
Agrobacterium tumefaciens
Cu2+
strong inhibition
Agrobacterium tumefaciens
EDTA
-
Agrobacterium tumefaciens
Hg2+
slight inhibition
Agrobacterium tumefaciens
Zn2+
slight inhibition
Agrobacterium tumefaciens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
2-amino-3-ureidopropionic acid
-
Agrobacterium tumefaciens
2.14
-
3-ureidopropionic acid
-
Agrobacterium tumefaciens
3.44
-
3-methyl-3-ureidopropionic acid
-
Agrobacterium tumefaciens
6.59
-
2-methyl-3-ureidopropionic acid
-
Agrobacterium tumefaciens
10.58
-
2-ureidoethanesulfonic acid
-
Agrobacterium tumefaciens
20.15
-
2-ureidoethane phosphonic acid
-
Agrobacterium tumefaciens
84.37
-
2-phenyl-3-ureidopropionic acid
-
Agrobacterium tumefaciens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
-
Agrobacterium tumefaciens
Mn2+
-
Agrobacterium tumefaciens
Ni2+
-
Agrobacterium tumefaciens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Agrobacterium tumefaciens
90000
-
gel filtration
Agrobacterium tumefaciens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Agrobacterium tumefaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens
3-aminoalanine + CO2 + NH3
-
-
-
?
2-amino-3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens C58 / ATCC 33970
3-aminoalanine + CO2 + NH3
-
-
-
?
2-methyl-3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens
alpha-methyl-beta-alanine + CO2 + NH3
-
-
-
?
2-methyl-3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens C58 / ATCC 33970
alpha-methyl-beta-alanine + CO2 + NH3
-
-
-
?
2-phenyl-3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens
3-amino-2-phenylpropanoic acid + CO2 + NH3
-
-
-
?
2-phenyl-3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens C58 / ATCC 33970
3-amino-2-phenylpropanoic acid + CO2 + NH3
-
-
-
?
2-ureidoethane phosphonic acid + H2O
-
695782
Agrobacterium tumefaciens
ciliatine + CO2 + NH3
-
-
-
?
2-ureidoethane phosphonic acid + H2O
-
695782
Agrobacterium tumefaciens C58 / ATCC 33970
ciliatine + CO2 + NH3
-
-
-
?
2-ureidoethanesulfonic acid + H2O
-
695782
Agrobacterium tumefaciens
taurine + CO2 + NH3
-
-
-
?
3-methyl-3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens
beta-homoalanine + CO2 + NH3
-
-
-
?
3-ureidopropionic acid + H2O
-
695782
Agrobacterium tumefaciens
beta-alanine + CO2 + NH3
-
-
-
?
N-carbamoyl-beta-alanine + H2O
-
695782
Agrobacterium tumefaciens
beta-alanine + CO2 + NH3
-
-
-
?
N-carbamoyl-beta-alanine + H2O
-
695782
Agrobacterium tumefaciens C58 / ATCC 33970
beta-alanine + CO2 + NH3
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 45000, SDS-PAGE
Agrobacterium tumefaciens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Agrobacterium tumefaciens
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
after 60 min 20% activity
Agrobacterium tumefaciens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.13
-
2-phenyl-3-ureidopropionic acid
-
Agrobacterium tumefaciens
0.15
-
2-amino-3-ureidopropionic acid
-
Agrobacterium tumefaciens
0.2
-
3-methyl-3-ureidopropionic acid
-
Agrobacterium tumefaciens
0.23
-
2-ureidoethane phosphonic acid
-
Agrobacterium tumefaciens
5.84
-
2-ureidoethanesulfonic acid
-
Agrobacterium tumefaciens
24.39
-
2-methyl-3-ureidopropionic acid
-
Agrobacterium tumefaciens
25.71
-
3-ureidopropionic acid
-
Agrobacterium tumefaciens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Agrobacterium tumefaciens
Other publictions for EC 3.5.1.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733675
Lam
NMR-based urinalysis for rapid ...
Homo sapiens
Clin. Chim. Acta
440
201-204
2015
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1
1
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734810
Meijer
Publishers note. Identificatio ...
Homo sapiens
Nucleosides Nucleotides Nucleic Acids
33
639-645
2014
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1
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3
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1
1
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734855
Shen
Expression of a gene encoding ...
Solanum lycopersicum, Solanum lycopersicum CL5915, Solanum lycopersicum L4783
Physiol. Plant.
150
425-435
2014
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1
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733382
Van Kuilenburg
beta-Ureidopropionase deficien ...
Homo sapiens
Biochim. Biophys. Acta
1822
1096-1108
2012
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1
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6
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1
2
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1
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1
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3
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2
1
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1
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6
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2
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1
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3
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2
1
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3
3
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734844
Fidlerova
Contribution of the beta-ureid ...
Homo sapiens
Pharmacol. Rep.
64
1234-1242
2012
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1
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2
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720104
Martinez-Gomez
N-Carbamoyl-beta-alanine amido ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
J. Chromatogr. B
879
3277-3282
2011
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1
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21
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1
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20
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1
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26
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1
21
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21
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26
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1
21
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21
21
719454
Janowitz
The 3-ureidopropionase of Caen ...
Caenorhabditis elegans
FEBS J.
277
4100-4109
2010
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1
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1
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3
1
1
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1
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695782
Martinez-Gomez
Potential application of N-car ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58 / ATCC 33970
Appl. Environ. Microbiol.
75
514-520
2009
-
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1
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3
-
5
7
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3
2
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21
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1
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13
1
1
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1
7
1
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1
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3
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5
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7
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3
2
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1
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13
1
1
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1
7
1
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700487
Zrenner
A functional analysis of the p ...
Arabidopsis thaliana
New Phytol.
183
117-132
2009
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
1
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-
712679
Kuhara
Five cases of beta-ureidopropi ...
Homo sapiens
J. Mass Spectrom.
44
214-221
2009
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1
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1
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2
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1
1
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685395
Schnackerz
Amidohydrolases of the reducti ...
Arabidopsis thaliana, Bos taurus, Caenorhabditis elegans, Dictyostelium discoideum, Drosophila melanogaster, Euglena gracilis, Homo sapiens, Lachancea kluyveri, Pseudomonas putida, Rattus norvegicus, Zea mays
Biochim. Biophys. Acta
1784
431-444
2008
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5
6
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4
7
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13
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4
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2
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22
8
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3
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5
3
6
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4
7
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4
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2
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22
8
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Ogawa
beta-Ureidopropionase with N-c ...
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Ogawa
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Matthews
Regulation of N-carbamoyl-beta ...
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Traut
Pyrimidine catabolism: individ ...
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Kaspari
-
Repression of beta-ureidopropi ...
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Wasternack
Pyrimidine-degrading enzymes p ...
Clostridium uracilicum, Clostridium uracilicum M5-2, Euglena gracilis, Mus musculus, Rattus rattus
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Campbell
Reductive degradation of pyrim ...
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Enzymatic decarbamylation of c ...
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