BRENDA - Enzyme Database
show all sequences of 3.5.1.57

Crystallization and characterization of a novel aminopeptidase from Trichosporon cutaneum

Iwayama, A.; Kimura, T.; Adachi, O.; Ameyama, M.; Agric. Biol. Chem. 47, 2483-2493 (1983)
No PubMed abstract available

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
-
Cutaneotrichosporon cutaneum
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
weak
Cutaneotrichosporon cutaneum
2,2'-dipyridyl
-
Cutaneotrichosporon cutaneum
monoiodoacetate
weak
Cutaneotrichosporon cutaneum
NEM
-
Cutaneotrichosporon cutaneum
PCMB
weak
Cutaneotrichosporon cutaneum
PMSF
weak
Cutaneotrichosporon cutaneum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.6
-
L-Tryptophanamide
-
Cutaneotrichosporon cutaneum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Cutaneotrichosporon cutaneum
5829
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
some activation
Cutaneotrichosporon cutaneum
Mn2+
required for activity
Cutaneotrichosporon cutaneum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
4 * 68000, SDS-PAGE
Cutaneotrichosporon cutaneum
270000
-
gel filtration
Cutaneotrichosporon cutaneum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophanamide + H2O
Cutaneotrichosporon cutaneum
-
L-tryptophan + NH3
-
Cutaneotrichosporon cutaneum
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cutaneotrichosporon cutaneum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Cutaneotrichosporon cutaneum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1110
-
-
Cutaneotrichosporon cutaneum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-alaninamide + H2O
14% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-alanine + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-asparagine + H2O
22% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-aspartate + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-citrullin + H2O
22% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
?
-
-
-
?
L-glutamine + H2O
18% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-glutamate + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-leucinamide + H2O
26% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-leucine + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-methioninamide + H2O
14% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-methionine + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-phenylalaninamide + H2O
47% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-phenylalanine + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-serinamide + H2O
7% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-serine + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-tryptophanamide + H2O
-
81203
Cutaneotrichosporon cutaneum
L-tryptophan + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-tyrosinamide + H2O
22% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-tyrosine + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-valinamide + H2O
15% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-valine + NH3
-
81203
Cutaneotrichosporon cutaneum
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 68000, SDS-PAGE
Cutaneotrichosporon cutaneum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
45
crystalline enzyme
Cutaneotrichosporon cutaneum
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
35
60
crystalline enzyme, 35C: about 75% of activity maximum, 60C: about 35% of activity maximum
Cutaneotrichosporon cutaneum
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
crystalline enzyme, 10 min, stable
Cutaneotrichosporon cutaneum
60
-
crystalline enzyme, rapid inactivation
Cutaneotrichosporon cutaneum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
crystalline enzyme
Cutaneotrichosporon cutaneum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
8.5
crystalline enzyme, pH 7.5, 20 h, room temperature
Cutaneotrichosporon cutaneum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Cutaneotrichosporon cutaneum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
weak
Cutaneotrichosporon cutaneum
2,2'-dipyridyl
-
Cutaneotrichosporon cutaneum
monoiodoacetate
weak
Cutaneotrichosporon cutaneum
NEM
-
Cutaneotrichosporon cutaneum
PCMB
weak
Cutaneotrichosporon cutaneum
PMSF
weak
Cutaneotrichosporon cutaneum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.6
-
L-Tryptophanamide
-
Cutaneotrichosporon cutaneum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Cutaneotrichosporon cutaneum
5829
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
some activation
Cutaneotrichosporon cutaneum
Mn2+
required for activity
Cutaneotrichosporon cutaneum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
4 * 68000, SDS-PAGE
Cutaneotrichosporon cutaneum
270000
-
gel filtration
Cutaneotrichosporon cutaneum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophanamide + H2O
Cutaneotrichosporon cutaneum
-
L-tryptophan + NH3
-
Cutaneotrichosporon cutaneum
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Cutaneotrichosporon cutaneum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1110
-
-
Cutaneotrichosporon cutaneum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-alaninamide + H2O
14% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-alanine + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-asparagine + H2O
22% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-aspartate + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-citrullin + H2O
22% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
?
-
-
-
?
L-glutamine + H2O
18% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-glutamate + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-leucinamide + H2O
26% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-leucine + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-methioninamide + H2O
14% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-methionine + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-phenylalaninamide + H2O
47% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-phenylalanine + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-serinamide + H2O
7% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-serine + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-tryptophanamide + H2O
-
81203
Cutaneotrichosporon cutaneum
L-tryptophan + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-tyrosinamide + H2O
22% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-tyrosine + NH3
-
81203
Cutaneotrichosporon cutaneum
?
L-valinamide + H2O
15% of the activity with L-tryptophanamide
81203
Cutaneotrichosporon cutaneum
L-valine + NH3
-
81203
Cutaneotrichosporon cutaneum
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 68000, SDS-PAGE
Cutaneotrichosporon cutaneum
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
45
crystalline enzyme
Cutaneotrichosporon cutaneum
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
35
60
crystalline enzyme, 35C: about 75% of activity maximum, 60C: about 35% of activity maximum
Cutaneotrichosporon cutaneum
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
crystalline enzyme, 10 min, stable
Cutaneotrichosporon cutaneum
60
-
crystalline enzyme, rapid inactivation
Cutaneotrichosporon cutaneum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
crystalline enzyme
Cutaneotrichosporon cutaneum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7.5
8.5
crystalline enzyme, pH 7.5, 20 h, room temperature
Cutaneotrichosporon cutaneum
Other publictions for EC 3.5.1.57
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
668755
Adachi
-
Tryptophanyl aminopeptidase ...
Cutaneotrichosporon cutaneum
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
1
1014-1015
2004
-
1
-
-
-
-
3
-
1
2
2
-
-
1
-
-
-
-
-
-
-
-
12
1
1
-
2
-
1
-
1
-
-
1
-
-
1
-
-
-
-
-
-
3
-
-
1
2
2
-
-
-
-
-
-
-
-
-
12
1
1
-
2
-
1
-
1
1
-
-
-
-
-
-
209203
Murphy
-
Localization and characterizat ...
Arabidopsis sp.
Plant Physiol. Biochem.
37
431-443
1999
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
1
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
81203
Iwayama
-
Crystallization and characteri ...
Cutaneotrichosporon cutaneum
Agric. Biol. Chem.
47
2483-2493
1983
-
-
-
1
-
-
6
1
1
2
2
1
-
1
-
-
1
-
-
-
1
-
11
1
1
1
2
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
6
-
1
1
2
2
1
-
-
-
1
-
-
1
-
11
1
1
1
2
-
1
-
1
-
-
-
-
-
-
-