Cloned (Comment) | Organism |
---|---|
encoded on plasmid pLVM1, DNA and amino acid sequence determination and analysis, genetic organization, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Methylobacterium sp. DM1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight inhibition | Methylobacterium sp. DM1 | |
Co2+ | slight inhibition | Methylobacterium sp. DM1 | |
Cr2+ | slight inhibition | Methylobacterium sp. DM1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
27.6 | - |
N,N-Dimethylformamide | pH 6.5, 37°C, recombinant enzyme | Methylobacterium sp. DM1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | slight activation | Methylobacterium sp. DM1 | |
Fe2+ | slight activation | Methylobacterium sp. DM1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | - |
recombinant His6-tagged enzyme, native PAGE | Methylobacterium sp. DM1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N-dimethylformamide + H2O | Methylobacterium sp. DM1 | - |
dimethylamine + formate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylobacterium sp. DM1 | A0A2Y9CJ10 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Methylobacterium sp. DM1 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | strain DM1 can utilize N,N-dimethylformamide, DMA, MA, methanol, ethanol, formamide, toluene, glycerol, and trisodium citrate as the sole source of carbon or carbon and nitrogen | Methylobacterium sp. DM1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N-dimethylformamide + H2O | - |
Methylobacterium sp. DM1 | dimethylamine + formate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | recombinent enzyme, alpha2beta2, 2 * 20000, alpha-subunit, + 2 * 80000, beta-subunit, SDS-PAGE | Methylobacterium sp. DM1 |
More | secondary structure analysis | Methylobacterium sp. DM1 |
Synonyms | Comment | Organism |
---|---|---|
C0214_26885 | locus name | Methylobacterium sp. DM1 |
dmfA1 | - |
Methylobacterium sp. DM1 |
dmfA2 | - |
Methylobacterium sp. DM1 |
DMFase | - |
Methylobacterium sp. DM1 |
N,N-dimethylformamidase | - |
Methylobacterium sp. DM1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Methylobacterium sp. DM1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
recombinant enzyme | Methylobacterium sp. DM1 |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is important and initial in the N,N-dimethylformamide (DMF) degradation pathway, transcriptional analysis of genes specific to DMF degradation, overview | Methylobacterium sp. DM1 |
physiological function | the enzyme is responsible for N,N-dimethylformamide degradation, molecular mechanism, overview | Methylobacterium sp. DM1 |