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Literature summary for 3.5.1.56 extracted from

  • Veeranagouda, Y.; Emmanuel Paul, P.V.; Gorla, P.; Siddavattam, D.; Karegoudar, T.B.
    Complete mineralisation of dimethylformamide by Ochrobactrum sp. DGVK1 isolated from the soil samples collected from the coalmine leftovers (2006), Appl. Microbiol. Biotechnol., 71, 369-375.
    View publication on PubMed
Search for more literature for 3.5.1.56

Application

Application Comment Organism
environmental protection the enzyme activity is useful to treat industrial effluent containing dimethylformamide obtained from pharmaceutical industry Ochrobactrum sp.

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichai coli Ochrobactrum sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N,N-dimethylformamide + H2O Ochrobactrum sp. catabolic pathway and related enzymes, degradation by strain DGVK1 leads to accumulation of ammonia and dimethylamine, overview dimethylamine + formate
-
 ?
N,N-dimethylformamide + H2O Ochrobactrum sp. DGVK1 catabolic pathway and related enzymes, degradation by strain DGVK1 leads to accumulation of ammonia and dimethylamine, overview dimethylamine + formate
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 ?

Organism

Organism UniProt Comment Textmining
Ochrobactrum sp.
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 isolated from soil samples collected from coalmine leftovers
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Ochrobactrum sp.
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 DGVK1
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Ochrobactrum sp. DGVK1
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 isolated from soil samples collected from coalmine leftovers
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Ochrobactrum sp. DGVK1
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 DGVK1
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information strain DGVK1 is able to grow on N,N-dimethylformamide as sole carbon and nitrogen source, overview Ochrobactrum sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.481
-
 DMFase in cell-free extract of strain DGVK1 Ochrobactrum sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N-dimethylformamide + H2O
-
 Ochrobactrum sp. dimethylamine + formate
-
 ?
N,N-dimethylformamide + H2O catabolic pathway and related enzymes, degradation by strain DGVK1 leads to accumulation of ammonia and dimethylamine, overview Ochrobactrum sp. dimethylamine + formate
-
 ?
N,N-dimethylformamide + H2O
-
 Ochrobactrum sp. DGVK1 dimethylamine + formate
-
 ?
N,N-dimethylformamide + H2O catabolic pathway and related enzymes, degradation by strain DGVK1 leads to accumulation of ammonia and dimethylamine, overview Ochrobactrum sp. DGVK1 dimethylamine + formate
-
 ?

Synonyms

Synonyms Comment Organism
dimethylformamidase
-
 Ochrobactrum sp.
DMFase
-
 Ochrobactrum sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Ochrobactrum sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Ochrobactrum sp.