Purification and characterisation of long chain acyl aminoacylase from Penicillium sp. B-001

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

DTT

-

Penicillium sp.

Hg2+

95% inhibition

Penicillium sp.

PCMB

-

Penicillium sp.

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.21

-

N-oleoyl-L-glutamate

-

Penicillium sp.

0.21

-

N-oleolyl-L-glutamate

-

Penicillium sp.

Metals/Ions

Commentary

Organism

Structure

Ca2+

activates

Penicillium sp.

Cd2+

activates

Penicillium sp.

Cu2+

activates

Penicillium sp.

Mg2+

activates

Penicillium sp.

Ni2+

activates

Penicillium sp.

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

additional information

not: acetyl-L-glutamate

209199

Penicillium sp.

?

-

-

-

?

additional information

not: acetyl-L-glutamate

209199

Penicillium sp. B-001

?

-

-

-

?

N-arachidonoyl-L-glutamate + H2O

32% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp.

arachidonate + L-glutamate

-

209199

Penicillium sp.

?

N-benzoyl-L-glutamate + H2O

74% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp.

benzoate + L-glutamate

-

209199

Penicillium sp.

?

N-decanoyl-L-glutamate + H2O

best substrate

209199

Penicillium sp.

decanoate + L-glutamate

-

209199

Penicillium sp.

?

N-decanoyl-L-glutamate + H2O

best substrate

209199

Penicillium sp. B-001

decanoate + L-glutamate

-

209199

Penicillium sp. B-001

?

N-lauroyl-L-glutamate + H2O

88% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp.

lauroate + L-glutamate

-

209199

Penicillium sp.

?

N-lauroyl-L-glutamate + H2O

88% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp. B-001

lauroate + L-glutamate

-

209199

Penicillium sp. B-001

?

N-myristoyl-L-glutamate + H2O

-

209199

Penicillium sp.

myristoate + L-glutamate

-

209199

Penicillium sp.

?

N-myristoyl-L-glutamate + H2O

-

209199

Penicillium sp. B-001

myristoate + L-glutamate

-

209199

Penicillium sp. B-001

?

N-octanoyl-L-glutamate + H2O

80% of the activity with decanoyl-L-glutamate

209199

Penicillium sp.

octanoate + L-glutamate

-

209199

Penicillium sp.

?

N-oleolyl-L-glutamate + H2O

-

209199

Penicillium sp.

oleate + L-glutamate

-

-

-

?

N-oleoyl-L-glutamate + H2O

67% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp.

oleate + L-glutamate

-

209199

Penicillium sp.

?

N-palmitoyl-L-glutamate + H2O

49% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp.

palmitate + L-glutamate

-

-

-

?

N-palmitoyl-L-glutamate + H2O

49% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp. B-001

palmitate + L-glutamate

-

-

-

?

N-stearoyl-L-glutamate + H2O

67% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp.

stearate + L-glutamate

-

209199

Penicillium sp.

?

Inhibitors

Commentary

Organism

Structure

DTT

-

Penicillium sp.

Hg2+

95% inhibition

Penicillium sp.

PCMB

-

Penicillium sp.

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.21

-

N-oleoyl-L-glutamate

-

Penicillium sp.

0.21

-

N-oleolyl-L-glutamate

-

Penicillium sp.

Metals/Ions

Commentary

Organism

Structure

Ca2+

activates

Penicillium sp.

Cd2+

activates

Penicillium sp.

Cu2+

activates

Penicillium sp.

Mg2+

activates

Penicillium sp.

Ni2+

activates

Penicillium sp.

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

additional information

not: acetyl-L-glutamate

209199

Penicillium sp.

?

-

-

-

?

additional information

not: acetyl-L-glutamate

209199

Penicillium sp. B-001

?

-

-

-

?

N-arachidonoyl-L-glutamate + H2O

32% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp.

arachidonate + L-glutamate

-

209199

Penicillium sp.

?

N-benzoyl-L-glutamate + H2O

74% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp.

benzoate + L-glutamate

-

209199

Penicillium sp.

?

N-decanoyl-L-glutamate + H2O

best substrate

209199

Penicillium sp.

decanoate + L-glutamate

-

209199

Penicillium sp.

?

N-decanoyl-L-glutamate + H2O

best substrate

209199

Penicillium sp. B-001

decanoate + L-glutamate

-

209199

Penicillium sp. B-001

?

N-lauroyl-L-glutamate + H2O

88% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp.

lauroate + L-glutamate

-

209199

Penicillium sp.

?

N-lauroyl-L-glutamate + H2O

88% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp. B-001

lauroate + L-glutamate

-

209199

Penicillium sp. B-001

?

N-myristoyl-L-glutamate + H2O

-

209199

Penicillium sp.

myristoate + L-glutamate

-

209199

Penicillium sp.

?

N-myristoyl-L-glutamate + H2O

-

209199

Penicillium sp. B-001

myristoate + L-glutamate

-

209199

Penicillium sp. B-001

?

N-octanoyl-L-glutamate + H2O

80% of the activity with decanoyl-L-glutamate

209199

Penicillium sp.

octanoate + L-glutamate

-

209199

Penicillium sp.

?

N-oleolyl-L-glutamate + H2O

-

209199

Penicillium sp.

oleate + L-glutamate

-

-

-

?

N-oleoyl-L-glutamate + H2O

67% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp.

oleate + L-glutamate

-

209199

Penicillium sp.

?

N-palmitoyl-L-glutamate + H2O

49% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp.

palmitate + L-glutamate

-

-

-

?

N-palmitoyl-L-glutamate + H2O

49% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp. B-001

palmitate + L-glutamate

-

-

-

?

N-stearoyl-L-glutamate + H2O

67% of the activity with N-decanoyl-L-glutamate

209199

Penicillium sp.

stearate + L-glutamate

-

209199

Penicillium sp.

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

209199

Murase

-

Purification and characterisat ...

Penicillium sp., Penicillium sp. B-001

Biosci. Biotechnol. Biochem.

57

854-855

1993

-

-

-

-

-

-

3

2

-

5

1

-

-

2

-

-

1

-

-

1

1

-

16

-

-

-

-

1

-

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

3

-

2

-

5

1

-

-

-

-

1

-

1

1

-

16

-

-

-

1

-

1

-

1

-

-

-

-

-

-

-

209198

Shintani

Isolation and characterization ...

Brevundimonas diminuta

J. Biochem.

96

637-643

1984

4

-

-

-

-

-

3

6

-

-

3

-

-

1

-

-

1

-

-

-

1

-

10

2

-

1

-

2

-

3

-

1

-

-

-

-

4

-

-

-

-

-

-

-

3

-

6

-

-

3

-

-

-

-

1

-

-

1

-

10

2

1

-

2

-

3

-

1

-

-

-

-

-

-

-

209197

Fukuda

A new enzyme: long acyl aminoa ...

Brevundimonas diminuta

J. Biochem.

91

1731-1738

1982

-

-

-

-

-

-

3

4

-

-

1

-

-

1

-

-

1

-

-

-

1

-

9

-

-

1

-

2

-

3

-

2

-

-

-

-

-

-

-

-

-

-

-

-

3

-

4

-

-

1

-

-

-

-

1

-

-

1

-

9

-

1

-

2

-

3

-

2

-

-

-

-

-

-

-