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Literature summary for 3.5.1.54 extracted from

  • Yuvarajan, S.; Hameed, A.; Arun, A.B.; Saptami, K.; Rekha, P.D.
    Urease-negative uropathogen Kalamiella piersonii YU22 metabolizes urea by urea carboxylase and allophanate hydrolase enzyme system (2022), Microbiol. Res., 263, 127142.
    View publication on PubMed
Search for more literature for 3.5.1.54

Cloned(Commentary)

Cloned (Comment) Organism
gene atzF, qualitative and quantitative RT-PCR enzyme expression analysis Pantoea piersonii

Protein Variants

Protein Variants Comment Organism
additional information significantly higher concentrations of extracellular NH4-N are detected in the cultures along with overexpression of urea carboxylase (UC) and allophanate hydrolase (AH) genes. The bacterium forms biofilm, and displays swimming and swarming motilities in presence of urea. Additional glucose supply to urea boosts the colonization but ameliorates the media alkalization and ammonification through suppression of gene expressions encoding UC and AH Pantoea piersonii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urea-1-carboxylate + H2O Pantoea piersonii
-
 2 CO2 + 2 NH3
-
 ?
urea-1-carboxylate + H2O Pantoea piersonii YU22
-
 2 CO2 + 2 NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pantoea piersonii A0AAJ5UBK2 Kalamiella piersonii strain YU22 / MCC3118
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Pantoea piersonii YU22 A0AAJ5UBK2 Kalamiella piersonii strain YU22 / MCC3118
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Source Tissue

Source Tissue Comment Organism Textmining
additional information Growth kinetics of YU22 cells in M9 media containing urea, urea + glucose, L-asparagine, L-asparagine + glucose and glucose alone, overview Pantoea piersonii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urea-1-carboxylate + H2O
-
 Pantoea piersonii 2 CO2 + 2 NH3
-
 ?
urea-1-carboxylate + H2O
-
 Pantoea piersonii YU22 2 CO2 + 2 NH3
-
 ?

Synonyms

Synonyms Comment Organism
AtzF
-
 Pantoea piersonii

General Information

General Information Comment Organism
metabolism presence of an alternate enzyme system for urea breakdown namely urea carboxylase (UC, EC 6.3.4.6) and allophanate hydrolase (AH) in comparison to the major pathway through the activity of the enzyme urease. The urease-negative strain YU22 can utilize the UC and AH system for urea metabolism. Altered gene expression shows the evidence for active UC and AH pathway for urea degradation, overview. Significant higher expression of UC (7.6-26.7fold), and AH (18.7-39.3-old) genes are found in the presence of urea during 24-72 h of incubation, whereas, their expressions declines marginally at 96 h Pantoea piersonii
physiological function many urease-negative pathogenic bacteria may utilize the urea carboxylase (UC) and allophanate hydrolase (AH) enzyme system to gain advantages for their survival in different host conditions Pantoea piersonii