Cloned (Comment) | Organism |
---|---|
gene atzF, expression of the His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
K91A | site-directed mutagenesis, inactive mutant | Pseudomonas sp. |
S165A | site-directed mutagenesis, inactive mutant | Pseudomonas sp. |
S189A | site-directed mutagenesis, inactive mutant | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phenyl phosphorodiamidate | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
allophanate | pH 9.5, recombinant enzyme | Pseudomonas sp. | |
5.3 | - |
malonamic acid | pH 9.5, recombinant enzyme | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66223 | - |
4 * 66223, sequence calculation | Pseudomonas sp. |
260000 | - |
gel filtration, recombinant enzyme | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
allophanate + H2O | Pseudomonas sp. | - |
NH3 + CO2 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | Q936X2 | gene atzF | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
allophanate + H2O | - |
Pseudomonas sp. | NH3 + CO2 | - |
? | |
allophanate + H2O | - |
Pseudomonas sp. | NH3 + CO2 | - |
ir | |
biuret + H2O | - |
Pseudomonas sp. | ? | - |
? | |
malonamic acid + H2O | - |
Pseudomonas sp. | ? | - |
? | |
malonamic acid + hydroxylamine | hydroxylamine trapping activity | Pseudomonas sp. | malonohydroxamate + ? | - |
? | |
additional information | substrate specificity, no activity with methyl allophanate, hydantoic acid, oxamic acid, hydroxyurea, methyl carbamate, N-methylurea, acetylurea, 1-acetyl-2-thiourea, and semicarbazide, no activity with rhodanine, rhodanine-3-acetic acid, 3-aminorhodanine, (4R)-(-)-2-thioxo-4-thiazolidinecarboxylic acid, (-)-2-oxo-4-thiazolinecarboxylic acid, and 2-amino-5-bromothiazol | Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 66223, sequence calculation | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
AtzF | - |
Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
malonamic acid | pH 9.5, recombinant enzyme | Pseudomonas sp. | |
16.4 | - |
allophanate | pH 9.5, recombinant enzyme | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
recombinant enzyme | Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 11.3 | recombinant enzyme | Pseudomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
phenyl phosphorodiamidate | pH 9.5, recombinant enzyme | Pseudomonas sp. |