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Literature summary for 3.5.1.54 extracted from
- Allophanate hydrolase of Oleomonas sagaranensis involved in an ATP-dependent degradation pathway specific to urea (2005), FEMS Microbiol. Lett., 245, 61-65.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli strain BL21(DE3) | Oleomonas sagaranensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.042 | - |
allophanate | pH 9.5, recombinant enzyme | Oleomonas sagaranensis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61999 | - |
2 * 61999, sequence calculation | Oleomonas sagaranensis |
| 138000 | - |
gel filtration, recombinant enzyme | Oleomonas sagaranensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allophanate + H2O | Oleomonas sagaranensis | the enzyme forms an alternative urea degradation pathway with the urea carboxylase, EC 6.3.4.6 | NH3 + CO2 | - |
ir | |
| allophanate + H2O | Oleomonas sagaranensis HD-1 | the enzyme forms an alternative urea degradation pathway with the urea carboxylase, EC 6.3.4.6 | NH3 + CO2 | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oleomonas sagaranensis | - |
isolated from an oil field in Sagara, Japan | - |
| Oleomonas sagaranensis HD-1 | - |
isolated from an oil field in Sagara, Japan | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) by ultracentrifucation, ion exchange chromatography, and gel filtration, 2.8fold to homogeneity | Oleomonas sagaranensis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| urea-1-carboxylate + H2O = 2 CO2 + 2 NH3 | the catalytic triad consists of residues Ser177, Ser153, and Lys79 | Oleomonas sagaranensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 108 | - |
purified recombinant enzyme, substrate allophanate | Oleomonas sagaranensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allophanate + H2O | the enzyme forms an alternative urea degradation pathway with the urea carboxylase, EC 6.3.4.6 | Oleomonas sagaranensis | NH3 + CO2 | - |
ir | |
| allophanate + H2O | - |
Oleomonas sagaranensis | NH3 + CO2 | - |
ir | |
| allophanate + H2O | the enzyme forms an alternative urea degradation pathway with the urea carboxylase, EC 6.3.4.6 | Oleomonas sagaranensis HD-1 | NH3 + CO2 | - |
ir | |
| allophanate + H2O | - |
Oleomonas sagaranensis HD-1 | NH3 + CO2 | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 61999, sequence calculation | Oleomonas sagaranensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Oleomonas sagaranensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Oleomonas sagaranensis |
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