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Literature summary for 3.5.1.54 extracted from

  • Shapir, N.; Cheng, G.; Sadowsky, M.J.; Wackett, L.P.
    Purification and characterization of TrzF: biuret hydrolysis by allophanate hydrolase supports growth (2006), Appl. Environ. Microbiol., 72, 2491-2495.
    View publication on PubMed
Search for more literature for 3.5.1.54

Cloned(Commentary)

Cloned (Comment) Organism
gene trzF, overexpression of His-tagged enzyme in presence of a chaperone in Escherichia coli strain BL21(DE3) Enterobacter cloacae

Inhibitors

Inhibitors Comment Organism Structure
phenyl phosphorodiamidate
-
 Enterobacter cloacae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.68
-
 allophanate pH 7.4, 25°C, recombinant enzyme Enterobacter cloacae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme contains no metal ions Enterobacter cloacae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65401
-
 2 * 68000, His-tagged recombinant enzyme, SDS-PAGE, 2 * 65401, sequence calculation Enterobacter cloacae
68000
-
 2 * 68000, His-tagged recombinant enzyme, SDS-PAGE, 2 * 65401, sequence calculation Enterobacter cloacae
144000
-
 His-tagged recombinant enzyme, gel filtration Enterobacter cloacae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
allophanate + H2O Enterobacter cloacae
-
 NH3 + CO2
-
 ir
allophanate + H2O Enterobacter cloacae 99
-
 NH3 + CO2
-
 ir

Organism

Organism UniProt Comment Textmining
Enterobacter cloacae Q9ALV2 gene trzF
-
Enterobacter cloacae 99 Q9ALV2 gene trzF
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.3
-
 purified recombinant enzyme Enterobacter cloacae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
allophanate + H2O
-
 Enterobacter cloacae NH3 + CO2
-
 ir
allophanate + H2O
-
 Enterobacter cloacae NH3 + CO2
-
 ?
allophanate + H2O
-
 Enterobacter cloacae 99 NH3 + CO2
-
 ir
allophanate + H2O
-
 Enterobacter cloacae 99 NH3 + CO2
-
 ?
biuret + H2O
-
 Enterobacter cloacae ?
-
 ?
biuret + H2O
-
 Enterobacter cloacae 99 ?
-
 ?
malonamic acid + H2O
-
 Enterobacter cloacae ?
-
 ?
malonamic acid + H2O
-
 Enterobacter cloacae 99 ?
-
 ?
malonamide + H2O
-
 Enterobacter cloacae ?
-
 ?
malonamide + H2O
-
 Enterobacter cloacae 99 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 68000, His-tagged recombinant enzyme, SDS-PAGE, 2 * 65401, sequence calculation Enterobacter cloacae

Synonyms

Synonyms Comment Organism
AtzF
-
 Enterobacter cloacae
TrzF
-
 Enterobacter cloacae
urea amidolyase
-
 Enterobacter cloacae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Enterobacter cloacae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6
-
 allophanate pH 7.4, 25°C, recombinant enzyme Enterobacter cloacae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Enterobacter cloacae