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Literature summary for 3.5.1.54 extracted from
- Purification and characterization of TrzF: biuret hydrolysis by allophanate hydrolase supports growth (2006), Appl. Environ. Microbiol., 72, 2491-2495.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene trzF, overexpression of His-tagged enzyme in presence of a chaperone in Escherichia coli strain BL21(DE3) | Enterobacter cloacae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phenyl phosphorodiamidate | - |
Enterobacter cloacae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.68 | - |
allophanate | pH 7.4, 25°C, recombinant enzyme | Enterobacter cloacae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme contains no metal ions | Enterobacter cloacae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65401 | - |
2 * 68000, His-tagged recombinant enzyme, SDS-PAGE, 2 * 65401, sequence calculation | Enterobacter cloacae |
| 68000 | - |
2 * 68000, His-tagged recombinant enzyme, SDS-PAGE, 2 * 65401, sequence calculation | Enterobacter cloacae |
| 144000 | - |
His-tagged recombinant enzyme, gel filtration | Enterobacter cloacae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allophanate + H2O | Enterobacter cloacae | - |
NH3 + CO2 | - |
ir | |
| allophanate + H2O | Enterobacter cloacae 99 | - |
NH3 + CO2 | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterobacter cloacae | Q9ALV2 | gene trzF | - |
| Enterobacter cloacae 99 | Q9ALV2 | gene trzF | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.3 | - |
purified recombinant enzyme | Enterobacter cloacae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allophanate + H2O | - |
Enterobacter cloacae | NH3 + CO2 | - |
? | |
| allophanate + H2O | - |
Enterobacter cloacae | NH3 + CO2 | - |
ir | |
| allophanate + H2O | - |
Enterobacter cloacae 99 | NH3 + CO2 | - |
? | |
| allophanate + H2O | - |
Enterobacter cloacae 99 | NH3 + CO2 | - |
ir | |
| biuret + H2O | - |
Enterobacter cloacae | ? | - |
? | |
| biuret + H2O | - |
Enterobacter cloacae 99 | ? | - |
? | |
| malonamic acid + H2O | - |
Enterobacter cloacae | ? | - |
? | |
| malonamic acid + H2O | - |
Enterobacter cloacae 99 | ? | - |
? | |
| malonamide + H2O | - |
Enterobacter cloacae | ? | - |
? | |
| malonamide + H2O | - |
Enterobacter cloacae 99 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 68000, His-tagged recombinant enzyme, SDS-PAGE, 2 * 65401, sequence calculation | Enterobacter cloacae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtzF | - |
Enterobacter cloacae |
| TrzF | - |
Enterobacter cloacae |
| urea amidolyase | - |
Enterobacter cloacae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Enterobacter cloacae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6 | - |
allophanate | pH 7.4, 25°C, recombinant enzyme | Enterobacter cloacae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Enterobacter cloacae |
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