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Literature summary for 3.5.1.54 extracted from
- Allophanate hydrolase, not urease, functions in bacterial cyanuric acid metabolism (2005), Appl. Environ. Microbiol., 71, 4437-4445.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| sequence analysis and comparison, and phylogenetic analysis | Agrobacterium tumefaciens |
| sequence analysis and comparison, and phylogenetic analysis | Enterobacter cloacae |
| sequence analysis and comparison, and phylogenetic analysis | Herbaspirillum huttiense |
| sequence analysis and comparison, and phylogenetic analysis | Ralstonia pickettii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allophanate + H2O | Pseudomonas sp. | the enzyme is involved in bacterial cyanuric acid metabolism | NH3 + CO2 | - |
ir |  |
| allophanate + H2O | Ralstonia pickettii | the enzyme is involved in bacterial cyanuric acid metabolism | NH3 + CO2 | - |
ir | |
| allophanate + H2O | Agrobacterium tumefaciens | the enzyme is involved in bacterial cyanuric acid metabolism | NH3 + CO2 | - |
ir | |
| allophanate + H2O | Herbaspirillum huttiense | the enzyme is involved in bacterial cyanuric acid metabolism | NH3 + CO2 | - |
ir | |
| allophanate + H2O | Enterobacter cloacae | the enzyme is involved in bacterial cyanuric acid metabolism | NH3 + CO2 | - |
ir | |
| allophanate + H2O | Agrobacterium tumefaciens J14a | the enzyme is involved in bacterial cyanuric acid metabolism | NH3 + CO2 | - |
ir | |
| allophanate + H2O | Ralstonia pickettii D | the enzyme is involved in bacterial cyanuric acid metabolism | NH3 + CO2 | - |
ir | |
| allophanate + H2O | Enterobacter cloacae 99 | the enzyme is involved in bacterial cyanuric acid metabolism | NH3 + CO2 | - |
ir | |
| allophanate + H2O | Herbaspirillum huttiense NRRL B-12228 | the enzyme is involved in bacterial cyanuric acid metabolism | NH3 + CO2 | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium tumefaciens | - |
gene atzF | - |
| Agrobacterium tumefaciens J14a | - |
gene atzF | - |
| Enterobacter cloacae | Q9ALV2 | - |
- |
| Enterobacter cloacae 99 | Q9ALV2 | - |
- |
| Herbaspirillum huttiense | - |
gene atzF | - |
| Herbaspirillum huttiense NRRL B-12228 | - |
gene atzF | - |
| Pseudomonas sp. | Q936X2 | gene atzF | - |
| Ralstonia pickettii | - |
gene atzF | - |
| Ralstonia pickettii D | - |
gene atzF | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allophanate + H2O | the enzyme is involved in bacterial cyanuric acid metabolism | Pseudomonas sp. | NH3 + CO2 | - |
ir | |
| allophanate + H2O | the enzyme is involved in bacterial cyanuric acid metabolism | Ralstonia pickettii | NH3 + CO2 | - |
ir | |
| allophanate + H2O | the enzyme is involved in bacterial cyanuric acid metabolism | Agrobacterium tumefaciens | NH3 + CO2 | - |
ir | |
| allophanate + H2O | the enzyme is involved in bacterial cyanuric acid metabolism | Herbaspirillum huttiense | NH3 + CO2 | - |
ir | |
| allophanate + H2O | the enzyme is involved in bacterial cyanuric acid metabolism | Enterobacter cloacae | NH3 + CO2 | - |
ir | |
| allophanate + H2O | - |
Pseudomonas sp. | NH3 + CO2 | - |
ir | |
| allophanate + H2O | - |
Ralstonia pickettii | NH3 + CO2 | - |
ir | |
| allophanate + H2O | - |
Agrobacterium tumefaciens | NH3 + CO2 | - |
ir | |
| allophanate + H2O | - |
Herbaspirillum huttiense | NH3 + CO2 | - |
ir | |
| allophanate + H2O | - |
Enterobacter cloacae | NH3 + CO2 | - |
ir | |
| allophanate + H2O | the enzyme is involved in bacterial cyanuric acid metabolism | Agrobacterium tumefaciens J14a | NH3 + CO2 | - |
ir | |
| allophanate + H2O | - |
Agrobacterium tumefaciens J14a | NH3 + CO2 | - |
ir | |
| allophanate + H2O | the enzyme is involved in bacterial cyanuric acid metabolism | Ralstonia pickettii D | NH3 + CO2 | - |
ir | |
| allophanate + H2O | - |
Ralstonia pickettii D | NH3 + CO2 | - |
ir | |
| allophanate + H2O | the enzyme is involved in bacterial cyanuric acid metabolism | Enterobacter cloacae 99 | NH3 + CO2 | - |
ir | |
| allophanate + H2O | - |
Enterobacter cloacae 99 | NH3 + CO2 | - |
ir | |
| allophanate + H2O | the enzyme is involved in bacterial cyanuric acid metabolism | Herbaspirillum huttiense NRRL B-12228 | NH3 + CO2 | - |
ir | |
| allophanate + H2O | - |
Herbaspirillum huttiense NRRL B-12228 | NH3 + CO2 | - |
ir | |
| additional information | allophanate also shows nonenzymatic decomposition, half-life at pH 8.0 is 50 h | Pseudomonas sp. | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtzF | - |
Pseudomonas sp. |
| AtzF | - |
Ralstonia pickettii |
| AtzF | - |
Agrobacterium tumefaciens |
| AtzF | - |
Herbaspirillum huttiense |
| AtzF | - |
Enterobacter cloacae |
| TrzF | - |
Enterobacter cloacae |
| urea amidolyase | - |
Enterobacter cloacae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Pseudomonas sp. |
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