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Literature summary for 3.5.1.54 extracted from
- Purification and properties of the allophanate hydrolase from Chlamydomonas reinhardii (1982), Biochim. Biophys. Acta, 714, 486-491.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Chlamydomonas reinhardtii |  |
| acetoacetic acid | Ki 0.3 mM | Chlamydomonas reinhardtii | |
| malonic acid | - |
Chlamydomonas reinhardtii | |
| NEM | allophanate protects | Chlamydomonas reinhardtii | |
| oxaloacetic acid | - |
Chlamydomonas reinhardtii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.55 | - |
allophanate | - |
Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
gel filtration | Chlamydomonas reinhardtii |
| 110000 | - |
PAGE | Chlamydomonas reinhardtii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allophanate + H2O | Chlamydomonas reinhardtii | 2nd step in the degradation of urea | CO2 + NH3 | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.3 | - |
- |
Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allophanate + H2O | - |
Chlamydomonas reinhardtii | CO2 + NH3 | - |
ir | |
| allophanate + H2O | 2nd step in the degradation of urea | Chlamydomonas reinhardtii | CO2 + NH3 | - |
ir | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Chlamydomonas reinhardtii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | pH 7.0: about 65% of maximal activity, pH 10.0: about 30% of maximal activity | Chlamydomonas reinhardtii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
acetoacetic acid | - |
Chlamydomonas reinhardtii | |
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