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Literature summary for 3.5.1.53 extracted from

  • Liao, S.; Poonpairoj, P.; Ko, K.; Takatuska, Y.; Yamaguchi, Y.; Abe, N.; Kaneko, J.; Kamio, Y.
    Occurrence of agmatine pathway for putrescine synthesis in Selenomonas ruminatium (2008), Biosci. Biotechnol. Biochem., 72, 445-455.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Selenomonas ruminantium
expression in Escherichia coli strain DE3 Selenomonas ruminantium

Protein Variants

Protein Variants Comment Organism
C153A inactive mutant enzyme Selenomonas ruminantium
C153A mutant without enzyme activity Selenomonas ruminantium
E160A inactive mutant enzyme Selenomonas ruminantium
E160A mutant without enzyme activity Selenomonas ruminantium

General Stability

General Stability Organism
in 50 mM PIPES buffer at pH 6.5 stable for 30 min up to 70°C Selenomonas ruminantium

Inhibitors

Inhibitors Comment Organism Structure
Co2+ strong inhibitor Selenomonas ruminantium
Fe2+ strong inhibitor Selenomonas ruminantium
Hg2+ strong inhibitor Selenomonas ruminantium
iodoacetamide strong inhibitor Selenomonas ruminantium
Mn2+ strong inhibitor Selenomonas ruminantium
Zn2+ strong inhibitor Selenomonas ruminantium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
N-Carbamoylputrescine
-
Selenomonas ruminantium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32532
-
2 * 32532, calculated from sequence Selenomonas ruminantium
37000
-
SDS-PAGE Selenomonas ruminantium
37000
-
2 * 37000, SDS-PAGE Selenomonas ruminantium
80000
-
gel filtration Selenomonas ruminantium

Organism

Organism UniProt Comment Textmining
Selenomonas ruminantium
-
-
-
Selenomonas ruminantium Q5KR04
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Selenomonas ruminantium
recombinant Selenomonas ruminantium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-carbamoylputrescine + H2O
-
Selenomonas ruminantium putrescine + CO2 + NH3
-
?
N-carbamoylputrescine + H2O 10 mM, assay at pH 6.5, 10 min, 40°C Selenomonas ruminantium putrescine + CO2 + NH3
-
?

Subunits

Subunits Comment Organism
dimer 2 * 37000, SDS-PAGE Selenomonas ruminantium
dimer 2 * 32532, calculated from sequence Selenomonas ruminantium
homodimer
-
Selenomonas ruminantium

Synonyms

Synonyms Comment Organism
AguB
-
Selenomonas ruminantium
N-carbamoyl-putrescine amidohydrolase
-
Selenomonas ruminantium
N-carbamoylputrescine amidohydrolase
-
Selenomonas ruminantium
NCP amidohydrolase
-
Selenomonas ruminantium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Selenomonas ruminantium
45
-
assay at pH 6.5, 10 min, 40°C Selenomonas ruminantium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
AguB activity is stable at up to 70°C for 30 min in 50 mM PIPES buffer, pH 6.5 Selenomonas ruminantium
70
-
up to 70°C, stable for 30 min at pH 6.5, 50 mM PIPES buffer Selenomonas ruminantium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Selenomonas ruminantium
7
-
assay at pH 6.5, 10 min, 40°C Selenomonas ruminantium

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8
-
Selenomonas ruminantium
6 8 stable Selenomonas ruminantium