Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Selenomonas ruminantium |
expression in Escherichia coli strain DE3 | Selenomonas ruminantium |
Protein Variants | Comment | Organism |
---|---|---|
C153A | inactive mutant enzyme | Selenomonas ruminantium |
C153A | mutant without enzyme activity | Selenomonas ruminantium |
E160A | inactive mutant enzyme | Selenomonas ruminantium |
E160A | mutant without enzyme activity | Selenomonas ruminantium |
General Stability | Organism |
---|---|
in 50 mM PIPES buffer at pH 6.5 stable for 30 min up to 70°C | Selenomonas ruminantium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | strong inhibitor | Selenomonas ruminantium | |
Fe2+ | strong inhibitor | Selenomonas ruminantium | |
Hg2+ | strong inhibitor | Selenomonas ruminantium | |
iodoacetamide | strong inhibitor | Selenomonas ruminantium | |
Mn2+ | strong inhibitor | Selenomonas ruminantium | |
Zn2+ | strong inhibitor | Selenomonas ruminantium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
N-Carbamoylputrescine | - |
Selenomonas ruminantium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32532 | - |
2 * 32532, calculated from sequence | Selenomonas ruminantium |
37000 | - |
SDS-PAGE | Selenomonas ruminantium |
37000 | - |
2 * 37000, SDS-PAGE | Selenomonas ruminantium |
80000 | - |
gel filtration | Selenomonas ruminantium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Selenomonas ruminantium | - |
- |
- |
Selenomonas ruminantium | Q5KR04 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Selenomonas ruminantium |
recombinant | Selenomonas ruminantium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbamoylputrescine + H2O | - |
Selenomonas ruminantium | putrescine + CO2 + NH3 | - |
? | |
N-carbamoylputrescine + H2O | 10 mM, assay at pH 6.5, 10 min, 40°C | Selenomonas ruminantium | putrescine + CO2 + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, SDS-PAGE | Selenomonas ruminantium |
dimer | 2 * 32532, calculated from sequence | Selenomonas ruminantium |
homodimer | - |
Selenomonas ruminantium |
Synonyms | Comment | Organism |
---|---|---|
AguB | - |
Selenomonas ruminantium |
N-carbamoyl-putrescine amidohydrolase | - |
Selenomonas ruminantium |
N-carbamoylputrescine amidohydrolase | - |
Selenomonas ruminantium |
NCP amidohydrolase | - |
Selenomonas ruminantium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Selenomonas ruminantium |
45 | - |
assay at pH 6.5, 10 min, 40°C | Selenomonas ruminantium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
AguB activity is stable at up to 70°C for 30 min in 50 mM PIPES buffer, pH 6.5 | Selenomonas ruminantium |
70 | - |
up to 70°C, stable for 30 min at pH 6.5, 50 mM PIPES buffer | Selenomonas ruminantium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Selenomonas ruminantium |
7 | - |
assay at pH 6.5, 10 min, 40°C | Selenomonas ruminantium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Selenomonas ruminantium |
6 | 8 | stable | Selenomonas ruminantium |