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Literature summary for 3.5.1.52 extracted from
- Evidence for an essential deglycosylation-independent activity of PNGase in Drosophila melanogaster (2010), PLoS ONE, 5, e10545.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Sf21 insect cells | Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C165T | inactive | Saccharomyces cerevisiae |
| C165T/N166V/R167C | inactive | Saccharomyces cerevisiae |
| C191A | inactive | Saccharomyces cerevisiae |
| N166V/R167C | the mutant shows 79% of wild type activity | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Drosophila melanogaster | 5829 | - |
| cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
x * 74000, SDS-PAGE | Drosophila melanogaster |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| larva | - |
Drosophila melanogaster | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the Drosophila ortholog of PNGase (PngI) does not possess deglycosylation activity but retains carbohydrate-binding activity. The transglutaminase domain of Pngl participates in some type of catalytic activity even though the protein does not have the conventional deglycosylation activity | Drosophila melanogaster | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 74000, SDS-PAGE | Drosophila melanogaster |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| peptide:N-glycanase | - |
Drosophila melanogaster |
| peptide:N-glycanase | - |
Saccharomyces cerevisiae |
| PNG1 | - |
Saccharomyces cerevisiae |
| PNGase | - |
Drosophila melanogaster |
| PNGase | - |
Saccharomyces cerevisiae |
| Pngl | - |
Drosophila melanogaster |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | homozygous deletion mutants show developmental defect | Drosophila melanogaster |
| physiological function | the enzyme plays a critical role during larval development and metamorphosis | Drosophila melanogaster |
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Release 2023.1 (January 2023)
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