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Literature summary for 3.5.1.52 extracted from

  • Hossain, M.; Nakano, R.; Nakamura, K.; Kimura, Y.
    Molecular identification and characterization of an acidic peptide:N-glycanase from tomato (Lycopersicum esculentum) fruits (2010), J. Biochem., 147, 157-165.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain JM109 and in Pichia pastoris strain GS115 Solanum lycopersicum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not require any metal ions for full enzymatic activity Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000, SDS-PAGE Solanum lycopersicum
65800
-
x * 65800, calculated from amino acid sequence Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Solanum lycopersicum the enzyme hydrolyses the beta-aspartyl-glycosylamine bond of N-linked glycoproteins/glycopeptides and releases free N-glycans ?
-
?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum D0QU16 cultivar Momotaro
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme possesses 10 N-glycosylation sites at positions of 220, 266, 386, 397, 447, 481, 489, 551, 556 and 570 in the deduced polypeptide chain Solanum lycopersicum

Purification (Commentary)

Purification (Comment) Organism
Ni-nitrilotriacetate column chromatography Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Cys-Gly-Leu-Val-Pro-Val-Leu-Ala-Glu-Asn-Tyr-Asn(Man3Gal2GlcNAc4NeuAc2)-Lys + H2O
-
Solanum lycopersicum ?
-
?
human transferrin glycopeptide + H2O
-
Solanum lycopersicum ?
-
?
additional information the enzyme hydrolyses the beta-aspartyl-glycosylamine bond of N-linked glycoproteins/glycopeptides and releases free N-glycans Solanum lycopersicum ?
-
?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Solanum lycopersicum
? x * 65800, calculated from amino acid sequence Solanum lycopersicum

Synonyms

Synonyms Comment Organism
acidic peptide:N-glycanase
-
Solanum lycopersicum
acidic PNGase
-
Solanum lycopersicum
glycoamidase
-
Solanum lycopersicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Solanum lycopersicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 enzyme activity declines rapidly above 40°C (80% activity at 50°C, 55% activity at 60°C, 30% activity at 70°C, no activity at 80°C) Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Solanum lycopersicum

pH Range

pH Minimum pH Maximum Comment Organism
3 6 enzyme activity declines rapidly above pH 6.0 Solanum lycopersicum

General Information

General Information Comment Organism
physiological function the enzyme is involved in a different de-N-glycosylation mechanism associated with plant growth and development Solanum lycopersicum