Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM109 and in Pichia pastoris strain GS115 | Solanum lycopersicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require any metal ions for full enzymatic activity | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, SDS-PAGE | Solanum lycopersicum |
65800 | - |
x * 65800, calculated from amino acid sequence | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Solanum lycopersicum | the enzyme hydrolyses the beta-aspartyl-glycosylamine bond of N-linked glycoproteins/glycopeptides and releases free N-glycans | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | D0QU16 | cultivar Momotaro | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme possesses 10 N-glycosylation sites at positions of 220, 266, 386, 397, 447, 481, 489, 551, 556 and 570 in the deduced polypeptide chain | Solanum lycopersicum |
Purification (Comment) | Organism |
---|---|
Ni-nitrilotriacetate column chromatography | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cys-Gly-Leu-Val-Pro-Val-Leu-Ala-Glu-Asn-Tyr-Asn(Man3Gal2GlcNAc4NeuAc2)-Lys + H2O | - |
Solanum lycopersicum | ? | - |
? | |
human transferrin glycopeptide + H2O | - |
Solanum lycopersicum | ? | - |
? | |
additional information | the enzyme hydrolyses the beta-aspartyl-glycosylamine bond of N-linked glycoproteins/glycopeptides and releases free N-glycans | Solanum lycopersicum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Solanum lycopersicum |
? | x * 65800, calculated from amino acid sequence | Solanum lycopersicum |
Synonyms | Comment | Organism |
---|---|---|
acidic peptide:N-glycanase | - |
Solanum lycopersicum |
acidic PNGase | - |
Solanum lycopersicum |
glycoamidase | - |
Solanum lycopersicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Solanum lycopersicum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | enzyme activity declines rapidly above 40°C (80% activity at 50°C, 55% activity at 60°C, 30% activity at 70°C, no activity at 80°C) | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Solanum lycopersicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | enzyme activity declines rapidly above pH 6.0 | Solanum lycopersicum |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in a different de-N-glycosylation mechanism associated with plant growth and development | Solanum lycopersicum |