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Literature summary for 3.5.1.52 extracted from

  • Maerz, S.; Funakoshi, Y.; Negishi, Y.; Suzuki, T.; Seiler, S.
    The Neurospora peptide:N-glycanase ortholog PNG1 is essential for cell polarity despite its lack of enzymatic activity (2010), J. Biol. Chem., 285, 2326-2332.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.52

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Neurospora crassa

Protein Variants

Protein Variants Comment Organism
A208C inactive Neurospora crassa
A208C/Y235H inactive Neurospora crassa
C221T mutant with more than 50% wild type activity Saccharomyces cerevisiae
D179E/P180A mutant with more than 50% wild type activity Saccharomyces cerevisiae
D208R/V209A mutant with 10-50% wild type activity Saccharomyces cerevisiae
E185R mutant with 10-50% wild type activity Saccharomyces cerevisiae
E185R/T186V mutant with 10-50% wild type activity Saccharomyces cerevisiae
E193D inactive Saccharomyces cerevisiae
E193D/W194F inactive Saccharomyces cerevisiae
F224Y mutant with more than 50% wild type activity Saccharomyces cerevisiae
G206D/L207I mutant with 10-50% wild type activity Saccharomyces cerevisiae
I181W mutant with more than 50% wild type activity Saccharomyces cerevisiae
I181W/K182Q inactive Saccharomyces cerevisiae
K182Q mutant with more than 50% wild type activity Saccharomyces cerevisiae
L198V mutant with more than 50% wild type activity Saccharomyces cerevisiae
L200M mutant with more than 50% wild type activity Saccharomyces cerevisiae
L200M/I201L mutant with more than 50% wild type activity Saccharomyces cerevisiae
N178T mutant with 10-50% wild type activity Saccharomyces cerevisiae
N214C/R215Q mutant with 10-50% wild type activity Saccharomyces cerevisiae
N266F/F227H mutant with more than 50% wild type activity Saccharomyces cerevisiae
R187K/K188R mutant with more than 50% wild type activity Saccharomyces cerevisiae
V219L mutant with 1-10% wild type activity Saccharomyces cerevisiae
W194F/C195A mutant with 10-50% wild type activity Saccharomyces cerevisiae
Y211W mutant with more than 50% wild type activity Saccharomyces cerevisiae
Y223I mutant with 10-50% wild type activity Saccharomyces cerevisiae
Y235H inactive Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-
Saccharomyces cerevisiae
-
-
-

Synonyms

Synonyms Comment Organism
peptide:N-glycanase
-
 Saccharomyces cerevisiae
peptide:N-glycanase
-
 Neurospora crassa
PNG1
-
 Saccharomyces cerevisiae
PNG1 wild type PNG1 has substitutions in essential catalytic amino acids, and its deglycosylation activity is lost Neurospora crassa
PNGase
-
 Saccharomyces cerevisiae
PNGase
-
 Neurospora crassa

General Information

General Information Comment Organism
malfunction PNG1 deletion results in strong polarity defects Neurospora crassa
physiological function the peptide:N-glycanase ortholog PNG1 is essential for cell polarity despite its lack of enzymatic activity, PNG1 is involved in cell wall integrity Neurospora crassa