Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | 10 mM, absence causes inactivation of L-929 PNGase, suggesting S-H group essential for enzyme activity | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Mus musculus | |
Fe3+ | - |
Mus musculus | |
fucose residue alpha-1-3-linked or alpha-1-6-linked to the proximal GlnNAc residue | complete inhibition | Mus musculus | |
Glycoasparagine | 1 mM causes 86% inhibition | Mus musculus | |
liberated glycan reaction product | causes 82% inhibition | Mus musculus | |
Monoiodoacetic acid | 2 mM causes 64% inhibition | Mus musculus | |
Monoiodoacetic acid | 5 mM causes 82% inhibition of the neutral PNGase M | Oryzias latipes | |
Zn2+ | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.114 | - |
Fetuin glycopeptide | - |
Mus musculus | |
0.871 | - |
asiolo-ovomucoid glycopeptide | - |
Elizabethkingia meningoseptica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
2 * 105000, homodimer, SDS-PAGE, 41% content of hydrophobic acids | Mus musculus |
212000 | - |
gel filtration | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Elizabethkingia meningoseptica | - |
- |
- |
Mus musculus | - |
- |
- |
Oryzias latipes | - |
medaka fish | - |
Purification (Comment) | Organism |
---|---|
460fold | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | C3H mouse-derived L-929 fibroblast cells | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
asiolo-ovomucoid glycopeptide + H2O | - |
Elizabethkingia meningoseptica | ? | - |
? | |
bovine fetuin glycopeptide + H2O | - |
Elizabethkingia meningoseptica | ? | - |
? | |
bovine fetuin glycopeptide + H2O | Leu-Asn(Man3Gal3GlcNAc5)-Asp-Ser-Arg | Mus musculus | ? | - |
? | |
fetuin glycopeptide + H2O | - |
Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 105000, homodimer, SDS-PAGE, 41% content of hydrophobic acids | Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Mus musculus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | - |
Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
rapidly reduced stability above 37°C | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
L-929 PNGase, brain PNGase, kidney PNGase | Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7 | - |
Mus musculus |