Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.52 extracted from

  • Suzuki, T.; Seko, A.; Kitajima, K.; Inoue, Y.; Inoue, S.
    Purification and enzymatic properties of peptide:N-glycanase from C3H mouse-derived L-929 fibroblast cells (1994), J. Biol. Chem., 269, 17611-17618.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
DTT 10 mM, absence causes inactivation of L-929 PNGase, suggesting S-H group essential for enzyme activity Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Mus musculus
Fe3+
-
Mus musculus
fucose residue alpha-1-3-linked or alpha-1-6-linked to the proximal GlnNAc residue complete inhibition Mus musculus
Glycoasparagine 1 mM causes 86% inhibition Mus musculus
liberated glycan reaction product causes 82% inhibition Mus musculus
Monoiodoacetic acid 2 mM causes 64% inhibition Mus musculus
Monoiodoacetic acid 5 mM causes 82% inhibition of the neutral PNGase M Oryzias latipes
Zn2+
-
Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.114
-
Fetuin glycopeptide
-
Mus musculus
0.871
-
asiolo-ovomucoid glycopeptide
-
Elizabethkingia meningoseptica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Mus musculus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
2 * 105000, homodimer, SDS-PAGE, 41% content of hydrophobic acids Mus musculus
212000
-
gel filtration Mus musculus

Organism

Organism UniProt Comment Textmining
Elizabethkingia meningoseptica
-
-
-
Mus musculus
-
-
-
Oryzias latipes
-
medaka fish
-

Purification (Commentary)

Purification (Comment) Organism
460fold Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast C3H mouse-derived L-929 fibroblast cells Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
asiolo-ovomucoid glycopeptide + H2O
-
Elizabethkingia meningoseptica ?
-
?
bovine fetuin glycopeptide + H2O
-
Elizabethkingia meningoseptica ?
-
?
bovine fetuin glycopeptide + H2O Leu-Asn(Man3Gal3GlcNAc5)-Asp-Ser-Arg Mus musculus ?
-
?
fetuin glycopeptide + H2O
-
Mus musculus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 105000, homodimer, SDS-PAGE, 41% content of hydrophobic acids Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Mus musculus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 37
-
Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
rapidly reduced stability above 37°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
L-929 PNGase, brain PNGase, kidney PNGase Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
6.8 7
-
Mus musculus