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Literature summary for 3.5.1.52 extracted from

  • Takahashi, N.; Nishibe, H.
    Some characteristics of a new glycopeptidase acting on aspartylglycosylamine linkages (1978), J. Biochem., 84, 1467-1473.
    View publication on PubMed
Search for more literature for 3.5.1.52

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Prunus amygdalus var. dulcis
Fe3+
-
 Prunus amygdalus var. dulcis
additional information no effect: thiol inhibitors, iodoacetamide, N-ethylmaleimide, actinomycete protease inhibitors, leupeptins, chymostatin, pepstatin, Mg2+, Ca2+, Mn2+, EDTA, L-cysteine, phenylmethylsulfonyl fluoride, gamma-D-gluconolactone, yeast mannan, triomannose Prunus amygdalus var. dulcis
Zn2+
-
 Prunus amygdalus var. dulcis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
 bromelain glycopeptide stem bromelain undecapeptide, glycopeptidase group C Prunus amygdalus var. dulcis

Organism

Organism UniProt Comment Textmining
Prunus amygdalus var. dulcis
-
 almond
-

Reaction

Reaction Comment Organism Reaction ID
Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc-Asn-Asp-Glu-Ser-Ser + H2O = Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc + Asn-Asp-Glu-Ser-Ser Asn-Asn(oligosaccharide)-Glu-Ser-Ser + H2O = Asn-Asp-Glu-Ser-Ser + 1-amino-N-acetylglucosamine-oligosaccharide, step 1, catalysed by the enzyme, 1-amino-N-acetylglucosamine-oligosaccharide + H2O = N-acetylglucosamine-oligosaccharide + NH3, step 2 Prunus amygdalus var. dulcis
a

Storage Stability

Storage Stability Organism
2 months at -20°C, activity loss less than 50% Prunus amygdalus var. dulcis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bromelain glycopeptide + H2O
-
 Prunus amygdalus var. dulcis ?
-
 ?
glycopeptide + H2O with 3-11 amino acid residues Prunus amygdalus var. dulcis ?
-
 ?
additional information
-
 Prunus amygdalus var. dulcis ?
-
 ?
pineapple stem bromelain glycopeptide + H2O Asn-Asn-(oligosaccharide)-Glu-Ser-Ser Prunus amygdalus var. dulcis ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
 Prunus amygdalus var. dulcis