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Literature summary for 3.5.1.51 extracted from

  • Chsugi, M.; Kahn, J.; Hensley, C.; Chew, S.; Barker, H.A.
    Metabolism of L-beta-lysine by a Pseudomonas. Purification and properties of a deacetylase-thiolesterase utilizing 4-acetamidobutyryl CoA and related compounds (1981), J. Biol. Chem., 256, 7642-7651.
    View publication on PubMed
Search for more literature for 3.5.1.51

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 10% inhibition at 0.5 mM Pseudomonas sp.
EDTA 26% inhibition at 62 mM Pseudomonas sp.
ZnCl2 30% inhibition at 0.5 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 5-acetamidovaleryl CoA
-
 Pseudomonas sp.
0.005 0.012 propionyl-CoA estimated from rates at lower and higher substrate concentration Pseudomonas sp.
0.007 0.02 butyryl-CoA estimated from rates at lower and higher substrate concentration Pseudomonas sp.
0.007 0.026 valeryl-CoA estimated from rates at lower and higher substrate concentration Pseudomonas sp.
0.016
-
 DL-3-aminobutyryl-CoA
-
 Pseudomonas sp.
0.02
-
 4-butyramidobutyryl-CoA
-
 Pseudomonas sp.
0.048
-
 3-acetamidopropionyl-CoA
-
 Pseudomonas sp.
0.05
-
 4-propionamidobutyryl-CoA
-
 Pseudomonas sp.
0.059
-
 beta-alanyl-CoA
-
 Pseudomonas sp.
0.077
-
 2-acetamidoacetyl-CoA
-
 Pseudomonas sp.
1
-
 acetoacetyl-CoA
-
 Pseudomonas sp.
1.5
-
 4-acetamidobutyryl-pantetheine
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36500
-
 8 * 36500, SDS-PAGE Pseudomonas sp.
275000
-
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-acetamidobutanoyl-CoA + H2O Pseudomonas sp.
-
 acetate + 4-aminobutanoyl-CoA
-
 ir

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
over 50fold Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, 10 mM potassium phosphate, pH 7, 40 mM KCl or -75°C, 2 years, 50% loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-acetamidoacetyl-CoA + H2O
-
 Pseudomonas sp. acetate + aminoacetyl-CoA
-
 ?
3-acetamidopropionyl-CoA + H2O
-
 Pseudomonas sp. acetate + 3-aminopropionyl-CoA
-
 ?
4-acetamidobutanoyl-CoA + H2O
-
 Pseudomonas sp. acetate + 4-aminobutanoyl-CoA
-
 ir
4-acetamidobutyryl pantetheine + H2O
-
 Pseudomonas sp. acetate + 4-aminobutyrylpantetheine
-
 ?
4-aminobutanoyl-CoA + H2O
-
 Pseudomonas sp. 4-aminobutanoate + coenzyme A
-
 ?
4-butyramidobutyryl-CoA + H2O
-
 Pseudomonas sp. butanoate + 4-aminobutyryl-CoA
-
 ?
4-propionamidobutyryl-CoA + H2O
-
 Pseudomonas sp. propionate + 4-aminobutyryl-CoA
-
 ?
5-acetamidovaleryl-CoA + H2O
-
 Pseudomonas sp. acetate + aminovaleryl-CoA
-
 ?
acetoacetyl-CoA + H2O
-
 Pseudomonas sp. ?
-
 ?
acetyl-CoA + H2O very poor substrate Pseudomonas sp. ?
-
 ?
beta-alanyl-CoA + H2O
-
 Pseudomonas sp. beta alanine + CoA
-
 ?
butyryl-CoA + H2O
-
 Pseudomonas sp. butanoate + CoA
-
 ?
DL-3-aminobutyryl-CoA + H2O
-
 Pseudomonas sp. DL-3-aminobutyrate + CoA
-
 ?
propionyl-CoA + H2O
-
 Pseudomonas sp. ?
-
 ?
valeryl-CoA + H2O
-
 Pseudomonas sp. valerate + CoA
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 36500, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.6 8.3
-
 Pseudomonas sp.