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Literature summary for 3.5.1.5 extracted from

  • Zhang, Q.; Zha, X.; Zhou, N.; Tian, Y.
    Preparation of crosslinked enzyme aggregates (CLEAs) of acid urease with urethanase activity and their application (2016), J. Basic Microbiol., 56, 422-431 .
    View publication on PubMed
Search for more literature for 3.5.1.5

Protein Variants

Protein Variants Comment Organism
additional information crosslinked enzyme aggregates of Providencia rettgeri urease (PRU-CLEAs) are prepared using genipin as crosslinking agent, method, overview. Optimal at 0.3 g/l of bovine serum albumin. The aggregates remove urea, but the treatment with PRU-CLEAs reveals no significant change of volatile flavor substances in Chinese rice wine. By using urea as the substrate, the values of Km and Vmax of free urease from Providencia rettgeri JN-B815 are estimated to be 5.99 mmol/l and 840 nmol/min, while those of immobilized urease are 13.54 mmol/l and 940 nmol/min, respectively. By using urethane as the substrate, the Km and Vmax value of free urethanase are determined to be 183.82 mmol/l and 970 nmol/min, while those of immobilized enzyme are 705.78 mmol/l and 650 nmol/min, respectively Providencia rettgeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Providencia rettgeri the enzyme exhibits not only urease activity, but also urethanase (EC 3.5.1.75) activity ?
-
 ?
additional information Providencia rettgeri JN-B815 the enzyme exhibits not only urease activity, but also urethanase (EC 3.5.1.75) activity ?
-
 ?
additional information Providencia rettgeri CGMCC 8326 the enzyme exhibits not only urease activity, but also urethanase (EC 3.5.1.75) activity ?
-
 ?
urea + H2O Providencia rettgeri
-
 CO2 + 2 NH3
-
 ?
urea + H2O Providencia rettgeri JN-B815
-
 CO2 + 2 NH3
-
 ?
urea + H2O Providencia rettgeri CGMCC 8326
-
 CO2 + 2 NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Providencia rettgeri A0A1U8X5K7
-
-
Providencia rettgeri CGMCC 8326 A0A1U8X5K7
-
-
Providencia rettgeri JN-B815 A0A1U8X5K7
-
-

Purification (Commentary)

Purification (Comment) Organism
native urease 2.9fold by ethanol precipitation, and anion exchange chromatography Providencia rettgeri

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.4
-
 purified enzyme, urease activity, pH 4.5, 37°C Providencia rettgeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme exhibits not only urease activity, but also urethanase (EC 3.5.1.75) activity Providencia rettgeri ?
-
 ?
additional information the enzyme exhibits not only urease activity, but also urethanase (EC 3.5.1.75) activity Providencia rettgeri JN-B815 ?
-
 ?
additional information the enzyme exhibits not only urease activity, but also urethanase (EC 3.5.1.75) activity Providencia rettgeri CGMCC 8326 ?
-
 ?
urea + H2O
-
 Providencia rettgeri CO2 + 2 NH3
-
 ?
urea + H2O
-
 Providencia rettgeri JN-B815 CO2 + 2 NH3
-
 ?
urea + H2O
-
 Providencia rettgeri CGMCC 8326 CO2 + 2 NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 61570, SDS-PAGE Providencia rettgeri

Synonyms

Synonyms Comment Organism
acid urease
-
 Providencia rettgeri
More also see for EC 3.5.1.75 Providencia rettgeri
UreC
-
 Providencia rettgeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Providencia rettgeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 assay at Providencia rettgeri