KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
Urea | pH 7.2, 37°C, soluble enzyme | Lysinibacillus sphaericus | |
3.33 | - |
Urea | pH 7.2, 37°C, immobilized enzyme | Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
185000 | - |
- |
Lysinibacillus sphaericus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | Lysinibacillus sphaericus | - |
CO2 + 2 NH3 | - |
? | |
urea + H2O | Lysinibacillus sphaericus MTCC 5100 | - |
CO2 + 2 NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
- |
- |
Lysinibacillus sphaericus MTCC 5100 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
salt precipitation followed by ion exchange chromatography followed by gel filtration chromatography | Lysinibacillus sphaericus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32.5 | - |
pH 7.2, 37°C | Lysinibacillus sphaericus |
Storage Stability | Organism |
---|---|
4°C, PBS buffer, pH 7.2, enzyme immobilized by cross linking using glutaraldehyde, about 50% loss of activity after 60 days | Lysinibacillus sphaericus |
4°C, PBS buffer, pH 7.2, soluble enzyme loses about 40% of its activity after 10 days | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
Lysinibacillus sphaericus | CO2 + 2 NH3 | - |
? | |
urea + H2O | - |
Lysinibacillus sphaericus MTCC 5100 | CO2 + 2 NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 70000 + 1 * 60000 + 1 * 55000, SDS-PAGE | Lysinibacillus sphaericus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Lysinibacillus sphaericus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 20°C: about 40% of maximal activity, 25°C: about 70% of maximal activity, 45°C: about 50% of maximal activity, 50°C: about 30% of maximal activity | Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Lysinibacillus sphaericus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5.0: about 60% of maximal activity, pH 8.0: about 60% of maximal activity | Lysinibacillus sphaericus |