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Literature summary for 3.5.1.5 extracted from

  • Singh, A.; Singh, M.; Verma, N.
    Extraction, purification, kinetic characterization and immobilization of urease from Bacillus sphaericus MTCC 5100 (2017), Biocatal. Agricult. Biotechnol., 12, 341-347 .
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
Urea pH 7.2, 37°C, soluble enzyme Lysinibacillus sphaericus
3.33
-
Urea pH 7.2, 37°C, immobilized enzyme Lysinibacillus sphaericus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
185000
-
-
Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urea + H2O Lysinibacillus sphaericus
-
CO2 + 2 NH3
-
?
urea + H2O Lysinibacillus sphaericus MTCC 5100
-
CO2 + 2 NH3
-
?

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
-
-
Lysinibacillus sphaericus MTCC 5100
-
-
-

Purification (Commentary)

Purification (Comment) Organism
salt precipitation followed by ion exchange chromatography followed by gel filtration chromatography Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32.5
-
pH 7.2, 37°C Lysinibacillus sphaericus

Storage Stability

Storage Stability Organism
4°C, PBS buffer, pH 7.2, enzyme immobilized by cross linking using glutaraldehyde, about 50% loss of activity after 60 days Lysinibacillus sphaericus
4°C, PBS buffer, pH 7.2, soluble enzyme loses about 40% of its activity after 10 days Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urea + H2O
-
Lysinibacillus sphaericus CO2 + 2 NH3
-
?
urea + H2O
-
Lysinibacillus sphaericus MTCC 5100 CO2 + 2 NH3
-
?

Subunits

Subunits Comment Organism
trimer 1 * 70000 + 1 * 60000 + 1 * 55000, SDS-PAGE Lysinibacillus sphaericus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Lysinibacillus sphaericus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 40% of maximal activity, 25°C: about 70% of maximal activity, 45°C: about 50% of maximal activity, 50°C: about 30% of maximal activity Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Lysinibacillus sphaericus

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 60% of maximal activity, pH 8.0: about 60% of maximal activity Lysinibacillus sphaericus