Crystallization (Comment) | Organism |
---|---|
free enzyme and enzyme in complex with inhibitor citrate, hanging drop vapour diffusion method, mixing of 0.001 ml of 11 mg/mL urease in 20 mM sodium N-(2-hydroxyethyl)piperazine-N'-ethanesulphonic acid sodium salt solution, pH 7.5, containing 50 mM Na2SO3, with 0.001 ml of the precipitant solution, equilibration by 1 ml of the precipitant solution, 20°C, the cryoprotectant solution contains 20% ethylene glycol, 2.4 M ammonium sulphate, 100 mM sodium citrate and 50 mM Na2SO3, X-ray diffraction structure determination and analysis at 1.5 A resolution | Sporosarcina pasteurii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | binding of the ligand to the active site involves stabilizing interactions, such as a carboxylate group that binds the nickel ions at the active site and several hydrogen bonds with the surrounding residues | Sporosarcina pasteurii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | dependent on, active site bound | Sporosarcina pasteurii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | Sporosarcina pasteurii | - |
CO2 + 2 NH3 | - |
? | |
urea + H2O | Sporosarcina pasteurii DSM 33 | - |
CO2 + 2 NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sporosarcina pasteurii | P41022 AND P41021 AND P41020 | P41022 i.e. subunit A, P41021 i.e. subunit B, P41020 i.e. subunit C | - |
Sporosarcina pasteurii DSM 33 | P41022 AND P41021 AND P41020 | P41022 i.e. subunit A, P41021 i.e. subunit B, P41020 i.e. subunit C | - |
Purification (Comment) | Organism |
---|---|
native enzyme by gel filtration and anion exchange chromatography | Sporosarcina pasteurii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
Sporosarcina pasteurii | CO2 + 2 NH3 | - |
? | |
urea + H2O | - |
Sporosarcina pasteurii DSM 33 | CO2 + 2 NH3 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Sporosarcina pasteurii |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyses the rapid hydrolysis of urea during the last step of the organic nitrogen mineralization | Sporosarcina pasteurii |
physiological function | the enzyme that catalyses the hydrolysis of urea, is a virulence factor for a large number of ureolytic bacterial human pathogens | Sporosarcina pasteurii |