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Literature summary for 3.5.1.5 extracted from

  • Contreras-Rodriguez, A.; Quiroz-Limon, J.; Martins, A.M.; Peralta, H.; Avila-Calderon, E.; Sriranganathan, N.; Boyle, S.M.; Lopez-Merino, A.
    Enzymatic, immunological and phylogenetic characterization of Brucella suis urease (2008), BMC Microbiol., 8, 121.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.5

Inhibitors

Inhibitors Comment Organism Structure
Acetohydroxamic acid
-
 Brucella suis bv. 1
Hydroxyurea
-
 Brucella suis bv. 1
Thiourea
-
 Brucella suis bv. 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6
-
 Urea by hyperbolic regression, Lineweaver-Burk: 5.24 mM Brucella suis bv. 1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11129
-
 x * 61054 + x * 11377 + x * 11129, calculated from sequence Brucella suis bv. 1
11377
-
 x * 61054 + x * 11377 + x * 11129, calculated from sequence Brucella suis bv. 1
14000
-
 x * 66000 + x * 15500 + x * 14000, SDS-PAGE Brucella suis bv. 1
15500
-
 x * 66000 + x * 15500 + x * 14000, SDS-PAGE Brucella suis bv. 1
61054
-
 x * 61054 + x * 11377 + x * 11129, calculated from sequence Brucella suis bv. 1
66000
-
 x * 66000 + x * 15500 + x * 14000, SDS-PAGE Brucella suis bv. 1
130000 135000 gel filtration Brucella suis bv. 1
157000
-
 nondenaturing PAGE Brucella suis bv. 1

Organism

Organism UniProt Comment Textmining
Brucella suis bv. 1 Q8FZW3 urease subunit beta 2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brucella suis bv. 1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
540
-
-
 Brucella suis bv. 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urea + H2O
-
 Brucella suis bv. 1 CO2 + 2 NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 61054 + x * 11377 + x * 11129, calculated from sequence Brucella suis bv. 1
? x * 66000 + x * 15500 + x * 14000, SDS-PAGE Brucella suis bv. 1

Synonyms

Synonyms Comment Organism
urease
-
 Brucella suis bv. 1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28 35
-
 Brucella suis bv. 1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 45
-
 Brucella suis bv. 1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
 Brucella suis bv. 1

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
 Brucella suis bv. 1

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.77
-
 Acetohydroxamic acid competitive Brucella suis bv. 1
1.04
-
 Hydroxyurea competitive Brucella suis bv. 1
26.12
-
 Thiourea competitive Brucella suis bv. 1

pI Value

Organism Comment pI Value Maximum pI Value
Brucella suis bv. 1
-
-
 5