Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methylophilus methylotrophus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
Formamide | - |
Methylophilus methylotrophus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Methylophilus methylotrophus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
alpha2, 2 * 51000, SDS-PAGE | Methylophilus methylotrophus |
123000 | - |
gel filtration | Methylophilus methylotrophus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylophilus methylotrophus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Methylophilus methylotrophus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | induction of enzyme synthesis using formamide- or acetamide-containing media | Methylophilus methylotrophus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37.4 | - |
- |
Methylophilus methylotrophus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetamide + H2O | very low activity | Methylophilus methylotrophus | acetic acid + NH3 | - |
? | |
acrylamide + H2O | very low activity | Methylophilus methylotrophus | acrylic acid + NH3 | - |
? | |
butyramide + H2O | very low activity | Methylophilus methylotrophus | butyric acid + NH3 | - |
? | |
formamide + H2O | - |
Methylophilus methylotrophus | formate + NH3 | source of nitrogen for growth and contribution to energy economy of the cell | ? | |
propionamide + H2O | very low activity | Methylophilus methylotrophus | propionic acid + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha2, 2 * 51000, SDS-PAGE | Methylophilus methylotrophus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60.7 | - |
Formamide | - |
Methylophilus methylotrophus |