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Literature summary for 3.5.1.49 extracted from
- Molecular characterization of formamidase from Methylophilus methylotrophus (1996), Eur. J. Biochem., 240, 314-322.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Thiourea | competitive inhibition, Ki: 4.6 mM | Methylophilus methylotrophus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44481 | - |
3 * 44481, electrospray mass spectrometry | Methylophilus methylotrophus |
| 133000 | 137000 | gel filtration, non-denaturing PAGE | Methylophilus methylotrophus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylophilus methylotrophus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Methylophilus methylotrophus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Methylophilus methylotrophus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formamide + H2O | - |
Methylophilus methylotrophus | formate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 44481, electrospray mass spectrometry | Methylophilus methylotrophus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.6 | - |
Thiourea | - |
Methylophilus methylotrophus | |
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Release 2023.1 (January 2023)
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