Cloned (Comment) | Organism |
---|---|
gene hdac10, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of TEV-cleavable N-terminal His-MBP-tagged enzyme from codon-optimized HDAC10 gene (residues 2-675) in Escherichia coli strain BL21(DE3), expression of wild-type and mutant enzymes | Danio rerio |
gene HDAC10, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of TEV-cleavable N-terminal His-MBP-tagged enzyme from codon-optimized HDAC10 gene in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified enzyme zHDAC10 mutant Y307F complexed with a polyamine transition state analogue inhibitor AAT, sitting drop vapour diffusion method, mixing of 400 nl of 30 mg/ml Y307F zHDAC10DELTA-AAT complex and 5 mM AAT with 400 nl of precipitant solution containing 0.2 M KH2PO4, and 20% PEG 3350, and equilibration against 0.08 ml precipitant solution, 24 h, 4°C, X-ray diffraction structure determination and analysis at 2.85 A resolution, molecular replacement using structure of the zHDAC6 CD1-TSA complex (PDB ID 5EEF) | Danio rerio |
Protein Variants | Comment | Organism |
---|---|---|
D94A | site-directed mutagenesis, steady-state kinetics | Danio rerio |
E274L | site-directed mutagenesis, steady-state kinetics, mutation of the glutamate gatekeeper residue | Danio rerio |
additional information | construction of deletion mutant DELTAnuA2, steady-state kinetics. The universal 310 helix nuA2 insertion in loop L1 further constricts the HDAC10 active site. Generation of mutant zHDAC10DELTA by treatement with trypsin | Danio rerio |
N93A | site-directed mutagenesis, steady-state kinetics | Danio rerio |
Y307F | site-directed mutagenesis, introduction of the deactivating Y307F mutation facilitates crystallization | Danio rerio |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7-[(3-aminopropyl)amino]-1,1,1-trifluoroheptan-2-one | AAT, enzyme-bound structure analysis, overview. This inhibitor binds as a transition state analogue | Danio rerio | |
7-[(3-aminopropyl)amino]-1,1,1-trifluoroheptan-2-one | AAT, the inhibitor binds as a transition state analogue | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Homo sapiens | |
0.01 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.017 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant D94A | Danio rerio | |
0.022 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.05 | - |
Acetylcadaverine | pH 8.0, 22°C, recombinant mutant N93A | Danio rerio | |
0.06 | - |
Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.06 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant deletion mutant DELTAnuA2 | Danio rerio | |
0.06 | - |
Acetylcadaverine | pH 8.0, 22°C, recombinant mutant E274L | Danio rerio | |
0.06 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant N93A | Danio rerio | |
0.066 | - |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.07 | - |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant E274L | Danio rerio | |
0.07 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant E274L | Danio rerio | |
0.08 | - |
Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.09 | - |
Acetylcadaverine | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.1 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.11 | - |
Acetylcadaverine | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.11 | - |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.11 | - |
acetylputrescine | pH 8.0, 22°C, recombinant mutant D94A | Danio rerio | |
0.11 | - |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant N93A | Danio rerio | |
0.13 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.13 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant mutant E274L | Danio rerio | |
0.13 | - |
acetylputrescine | pH 8.0, 22°C, recombinant mutant N93A | Danio rerio | |
0.14 | - |
Nalpha,Nepsilon-diacetyl-L-lysinamide | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.14 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant mutant D94A | Danio rerio | |
0.15 | - |
N1,N8-diacetylspermidine | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.15 | - |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant D94A | Danio rerio | |
0.15 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant mutant N93A | Danio rerio | |
0.16 | - |
acetylputrescine | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.16 | - |
N-(8-aminooctyl)acetamide | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.16 | - |
acetylputrescine | pH 8.0, 22°C, recombinant mutant E274L | Danio rerio | |
0.17 | - |
N-(8-aminooctyl)acetamide | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.17 | - |
acetylputrescine | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.18 | - |
N1,N8-diacetylspermidine | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.18 | - |
N1-acetylspermine | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.26 | - |
Acetylcadaverine | pH 8.0, 22°C, recombinant mutant D94A | Danio rerio | |
0.27 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant deletion mutant DELTAnuA2 | Danio rerio | |
0.9 | - |
N-butylacetamide | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | catalytic zinc ion, enzyme binding structure, overview | Homo sapiens | |
Zn2+ | catalytic zinc ion, enzyme binding structure, overview | Danio rerio |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N8-acetylspermidine + H2O | Homo sapiens | - |
acetate + spermidine | - |
? | |
N8-acetylspermidine + H2O | Danio rerio | - |
acetate + spermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | F1QCV2 | - |
- |
Homo sapiens | Q969S8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant TEV-cleavable N-terminal His-MBP-tagged enzyme from Escherichia coli strain BL21(DE3) by amylose affinity chromatography and anion exchange chromatography, the MBP-tag is not cleaved off | Homo sapiens |
recombinant TEV-cleavable N-terminal His-MBP-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by amylose affinity chromatography, TEV protease is used to remove the His-MBP-tag, followed by anion exchange chromatography and gel filtration | Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin + H2O | very low activity | Danio rerio | ? | - |
? | |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin + H2O | low activity | Homo sapiens | ? | - |
? | |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin + H2O | very low activity | Danio rerio | ? | - |
? | |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin + H2O | low activity | Homo sapiens | ? | - |
? | |
acetylcadaverine + H2O | high acitivity | Homo sapiens | acetate + cadaverine | - |
? | |
acetylcadaverine + H2O | high acitivity | Danio rerio | acetate + cadaverine | - |
? | |
acetylputrescine + H2O | high acitivity | Homo sapiens | acetate + putrescine | - |
? | |
acetylputrescine + H2O | high acitivity | Danio rerio | acetate + putrescine | - |
? | |
additional information | the conserved glutamate gatekeeper and a sterically constricted active site confer specificity for N8-acetylspermidine hydrolysis and disfavour acetyllysine hydrolysis. Analysis of the intermolecular interactions of N8-acetylspermidine in the active site of hHDAC10 based on the structure of the Danio rerio enzyme-inhibitor crystal structure zHDAC10-AAT complex. No activity with N-(3-aminopropyl)acetamide, TK(ac)PIW, AK(ac)P, GAK(ac), AK(ac), K(ac)NL, K(ac)NL, and GAK(ac)NLQ | Homo sapiens | ? | - |
? | |
additional information | zebrafish HDAC10 complexed with a transition-state analogue inhibitor reveals that a glutamate gatekeeper and a sterically constricted active site confer specificity for N8-acetylspermidine hydrolysis and disfavour acetyllysine hydrolysis. The N4 amino group of N8-acetylspermidine is recognized by a direct hydrogen bond with E117. No activity with TK(ac)PIW, AK(ac)P, GAK(ac), AK(ac), AK(ac)A, K(ac)NL, K(ac)NL, and GAK(ac)NLQ | Danio rerio | ? | - |
? | |
N-(3-aminopropyl)acetamide + H2O | very low activity | Danio rerio | propane-1,3-diamine + acetate | - |
? | |
N-(8-aminooctyl)acetamide + H2O | moderate activity | Homo sapiens | octane-1,8-diamine + acetate | - |
? | |
N-(8-aminooctyl)acetamide + H2O | moderate to high activity | Danio rerio | octane-1,8-diamine + acetate | - |
? | |
N-butylacetamide + H2O | very low activity | Danio rerio | butan-1-amine + acetate | - |
? | |
N-butylacetamide + H2O | low activity | Homo sapiens | butan-1-amine + acetate | - |
? | |
N1,N8-diacetylspermidine + 2 H2O | moderate activity | Homo sapiens | 2 acetate + spermidine | - |
? | |
N1,N8-diacetylspermidine + H2O | high activity | Danio rerio | 2 acetate + spermidine | - |
? | |
N1-acetylspermidine + H2O | very low activity | Danio rerio | acetate + spermidine | - |
? | |
N1-acetylspermine + H2O | very low activity | Danio rerio | acetate + spermine | - |
? | |
N1-acetylspermine + H2O | low activity | Homo sapiens | acetate + spermine | - |
? | |
N8-acetylspermidine + H2O | - |
Homo sapiens | acetate + spermidine | - |
? | |
N8-acetylspermidine + H2O | - |
Danio rerio | acetate + spermidine | - |
? | |
N8-acetylspermidine + H2O | best substrate | Homo sapiens | acetate + spermidine | - |
? | |
N8-acetylspermidine + H2O | best substrate | Danio rerio | acetate + spermidine | - |
? | |
Nalpha,Nepsilon-diacetyl-L-lysinamide + H2O | low activity | Homo sapiens | ? | - |
? | |
Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide + H2O | low activity | Homo sapiens | ? | - |
? | |
Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide + H2O | low activity | Danio rerio | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HDAC10 | - |
Homo sapiens |
HDAC10 | - |
Danio rerio |
histone deacetylase 10 | - |
Homo sapiens |
histone deacetylase 10 | - |
Danio rerio |
zHDAC10 | - |
Danio rerio |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
22 | - |
assay at room temperature | Danio rerio |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics of wild-type and mutant enzymes | Danio rerio | |
0.00007 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant N93A | Danio rerio | |
0.00033 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.00038 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.00046 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant D94A | Danio rerio | |
0.0006 | - |
Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.0013 | - |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant D94A | Danio rerio | |
0.0013 | - |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant N93A | Danio rerio | |
0.0015 | - |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.008 | - |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.0092 | - |
Nalpha,Nepsilon-diacetyl-L-lysinamide | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.011 | - |
N1-acetylspermine | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.013 | - |
Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.02 | - |
Acetylcadaverine | pH 8.0, 22°C, recombinant mutant E274L | Danio rerio | |
0.021 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant deletion mutant DELTAnuA2 | Danio rerio | |
0.028 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant mutant E274L | Danio rerio | |
0.034 | - |
acetylputrescine | pH 8.0, 22°C, recombinant mutant E274L | Danio rerio | |
0.051 | - |
Acetylcadaverine | pH 8.0, 22°C, recombinant mutant N93A | Danio rerio | |
0.053 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant E274L | Danio rerio | |
0.06 | - |
N-butylacetamide | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.082 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant deletion mutant DELTAnuA2 | Danio rerio | |
0.1 | - |
N-(8-aminooctyl)acetamide | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.123 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant mutant N93A | Danio rerio | |
0.126 | - |
acetylputrescine | pH 8.0, 22°C, recombinant mutant N93A | Danio rerio | |
0.14 | - |
Acetylcadaverine | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.14 | - |
N1,N8-diacetylspermidine | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.21 | - |
N-(8-aminooctyl)acetamide | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.21 | - |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant E274L | Danio rerio | |
0.24 | - |
acetylputrescine | pH 8.0, 22°C, recombinant mutant D94A | Danio rerio | |
0.28 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.29 | - |
Acetylcadaverine | pH 8.0, 22°C, recombinant mutant D94A | Danio rerio | |
0.35 | - |
acetylputrescine | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.37 | - |
Acetylcadaverine | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.48 | - |
N1,N8-diacetylspermidine | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.58 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.7 | - |
acetylputrescine | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.9 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant mutant D94A | Danio rerio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
8 | - |
assay at | Danio rerio |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
7-[(3-aminopropyl)amino]-1,1,1-trifluoroheptan-2-one | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.0007 | - |
7-[(3-aminopropyl)amino]-1,1,1-trifluoroheptan-2-one | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio |
General Information | Comment | Organism |
---|---|---|
physiological function | cationic polyamines such as spermidine and spermine are critical in all forms of life, as they regulate the function of biological macromolecules. Intracellular polyamine metabolism is regulated by reversible acetylation and dysregulated polyamine metabolism is associated with neoplastic diseases such as colon cancer, prostate cancer and neuroblastoma. Both HDAC10 and its product spermidine are known to promote cellular survival through autophagy | Homo sapiens |
physiological function | Cationic polyamines such as spermidine and spermine are critical in all forms of life, as they regulate the function of biological macromolecules. Intracellular polyamine metabolism is regulated by reversible acetylation. Both HDAC10 and its product spermidine are known to promote cellular survival through autophagy | Danio rerio |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant N93A | Danio rerio | |
0.0087 | - |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant D94A | Danio rerio | |
0.01 | - |
Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.012 | - |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant N93A | Danio rerio | |
0.015 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.023 | - |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.027 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant D94A | Danio rerio | |
0.038 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.061 | - |
N1-acetylspermine | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.066 | - |
Nalpha,Nepsilon-diacetyl-L-lysinamide | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.067 | - |
N-butylacetamide | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.072 | - |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.14 | - |
N1,N8-diacetylspermidine | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.163 | - |
Nalpha-acetyl-L-alanyl-Nepsilon-acetyl-L-lysyl-L-alaninamide | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
0.213 | - |
acetylputrescine | pH 8.0, 22°C, recombinant mutant E274L | Danio rerio | |
0.215 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant mutant E274L | Danio rerio | |
0.304 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant deletion mutant DELTAnuA2 | Danio rerio | |
0.333 | - |
Acetylcadaverine | pH 8.0, 22°C, recombinant mutant E274L | Danio rerio | |
0.35 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant deletion mutant DELTAnuA2 | Danio rerio | |
0.757 | - |
7-(Nalpha-acetyl-glycyl-L-alanyl-Nepsilon-acetyl-L-lysyl)amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant E274L | Danio rerio | |
0.82 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant mutant N93A | Danio rerio | |
0.93 | - |
N-(8-aminooctyl)acetamide | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
0.97 | - |
acetylputrescine | pH 8.0, 22°C, recombinant mutant N93A | Danio rerio | |
1.02 | - |
Acetylcadaverine | pH 8.0, 22°C, recombinant mutant N93A | Danio rerio | |
1.16 | - |
Acetylcadaverine | pH 8.0, 22°C, recombinant mutant D94A | Danio rerio | |
1.24 | - |
N-(8-aminooctyl)acetamide | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
1.27 | - |
Acetylcadaverine | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
2.06 | - |
acetylputrescine | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
2.18 | - |
acetylputrescine | pH 8.0, 22°C, recombinant mutant D94A | Danio rerio | |
2.8 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant enzyme | Homo sapiens | |
4.11 | - |
Acetylcadaverine | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
4.375 | - |
acetylputrescine | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
4.46 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant wild-type enzyme | Danio rerio | |
6.43 | - |
N8-Acetylspermidine | pH 8.0, 22°C, recombinant mutant D94A | Danio rerio | |
8.84 | - |
7-(Nalpha-acetyl-L-arginyl-glycyl-Nepsilon-acetyl-L-lysyl)-amino-4-methylcoumarin | pH 8.0, 22°C, recombinant mutant E274L | Danio rerio |