Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | weak activator | Azotobacter vinelandii | |
diphosphate | weak activator | Azotobacter vinelandii | |
phosphate | weak activator | Azotobacter vinelandii |
General Stability | Organism |
---|---|
DTT required during purification | Azotobacter vinelandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-nicotinic acid adenine dinucleotide | non-competitive inhibitor, Ki: 8 mM | Azotobacter vinelandii | |
alpha-nicotinic acid mononucleotide | non-competitive inhibitor, Ki: 21 mM | Azotobacter vinelandii | |
beta-nicotinic acid adenine dinucleotide | non-competitive inhibitor, Ki: 7 mM | Azotobacter vinelandii | |
Cu2+ | strong inhibitor | Azotobacter vinelandii | |
Isonicotinic acid | non-competitive inhibitor, Ki: 8.3 mM | Azotobacter vinelandii | |
nicotinic acid | non-competitive inhibitor, Ki: 4.4 mM | Azotobacter vinelandii | |
p-chloromercuribenzoate | - |
Azotobacter vinelandii | |
Picolinic acid | non-competitive inhibitor, Ki: 9.8 mM | Azotobacter vinelandii | |
quinolinic acid | non-competitive inhibitor, Ki: 3.9 mM | Azotobacter vinelandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.05 | - |
beta-nicotinamide D-ribonucleotide | - |
Azotobacter vinelandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | significant stimulation | Azotobacter vinelandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25700 | 26300 | gel filtration | Azotobacter vinelandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-nicotinamide D-ribonucleotide + H2O | Escherichia coli | - |
beta-nicotinate D-ribonucleotide + NH3 | - |
? | |
beta-nicotinamide D-ribonucleotide + H2O | Azotobacter vinelandii | involvement in NAD metabolism | beta-nicotinate D-ribonucleotide + NH3 | - |
ir | |
nicotinamide ribonucleoside + H2O | Azotobacter vinelandii | 14% activity compared to nicotinamide D-ribonucleotide | nicotinic acid ribonucleoside + NH3 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Escherichia coli | - |
- |
- |
no activity in Oryctolagus cuniculus | - |
- |
- |
no activity in Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Azotobacter vinelandii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Escherichia coli | - |
cell culture | - |
Azotobacter vinelandii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.22 | - |
- |
Azotobacter vinelandii |
Storage Stability | Organism |
---|---|
4°C, Tris-HCl, pH 7.5, 1 week | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-nicotinamide D-ribonucleotide + H2O | - |
Escherichia coli | beta-nicotinate D-ribonucleotide + NH3 | - |
? | |
beta-nicotinamide D-ribonucleotide + H2O | - |
Azotobacter vinelandii | beta-nicotinate D-ribonucleotide + NH3 | - |
ir | |
beta-nicotinamide D-ribonucleotide + H2O | involvement in NAD metabolism | Azotobacter vinelandii | beta-nicotinate D-ribonucleotide + NH3 | - |
ir | |
nicotinamide ribonucleoside + H2O | 14% activity compared to nicotinamide D-ribonucleotide | Azotobacter vinelandii | nicotinic acid ribonucleoside + NH3 | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
64 | - |
- |
Azotobacter vinelandii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
64 | - |
preincubation without substrate: complete denaturation within 32 min | Azotobacter vinelandii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.2 | - |
Azotobacter vinelandii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
quinolinic acid | - |
Azotobacter vinelandii | |
4.4 | - |
nicotinic acid | - |
Azotobacter vinelandii | |
7 | - |
beta-nicotinic acid adenine dinucleotide | - |
Azotobacter vinelandii | |
8 | - |
alpha-nicotinic acid adenine dinucleotide | - |
Azotobacter vinelandii | |
8.3 | - |
Isonicotinic acid | - |
Azotobacter vinelandii | |
9.8 | - |
Picolinic acid | - |
Azotobacter vinelandii | |
21 | - |
alpha-nicotinic acid mononucleotide | - |
Azotobacter vinelandii |