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Literature summary for 3.5.1.42 extracted from

  • Imai, T.
    Purification and properties of nicotinamide mononucleotide amidohydrolase from Azotobacter vinelandii (1973), J. Biochem., 73, 139-153.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP weak activator Azotobacter vinelandii
diphosphate weak activator Azotobacter vinelandii
phosphate weak activator Azotobacter vinelandii

General Stability

General Stability Organism
DTT required during purification Azotobacter vinelandii

Inhibitors

Inhibitors Comment Organism Structure
alpha-nicotinic acid adenine dinucleotide non-competitive inhibitor, Ki: 8 mM Azotobacter vinelandii
alpha-nicotinic acid mononucleotide non-competitive inhibitor, Ki: 21 mM Azotobacter vinelandii
beta-nicotinic acid adenine dinucleotide non-competitive inhibitor, Ki: 7 mM Azotobacter vinelandii
Cu2+ strong inhibitor Azotobacter vinelandii
Isonicotinic acid non-competitive inhibitor, Ki: 8.3 mM Azotobacter vinelandii
nicotinic acid non-competitive inhibitor, Ki: 4.4 mM Azotobacter vinelandii
p-chloromercuribenzoate
-
Azotobacter vinelandii
Picolinic acid non-competitive inhibitor, Ki: 9.8 mM Azotobacter vinelandii
quinolinic acid non-competitive inhibitor, Ki: 3.9 mM Azotobacter vinelandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.05
-
beta-nicotinamide D-ribonucleotide
-
Azotobacter vinelandii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ significant stimulation Azotobacter vinelandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25700 26300 gel filtration Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-nicotinamide D-ribonucleotide + H2O Escherichia coli
-
beta-nicotinate D-ribonucleotide + NH3
-
?
beta-nicotinamide D-ribonucleotide + H2O Azotobacter vinelandii involvement in NAD metabolism beta-nicotinate D-ribonucleotide + NH3
-
ir
nicotinamide ribonucleoside + H2O Azotobacter vinelandii 14% activity compared to nicotinamide D-ribonucleotide nicotinic acid ribonucleoside + NH3
-
ir

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Escherichia coli
-
-
-
no activity in Oryctolagus cuniculus
-
-
-
no activity in Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Azotobacter vinelandii

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Escherichia coli
-
cell culture
-
Azotobacter vinelandii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.22
-
-
Azotobacter vinelandii

Storage Stability

Storage Stability Organism
4°C, Tris-HCl, pH 7.5, 1 week Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-nicotinamide D-ribonucleotide + H2O
-
Escherichia coli beta-nicotinate D-ribonucleotide + NH3
-
?
beta-nicotinamide D-ribonucleotide + H2O
-
Azotobacter vinelandii beta-nicotinate D-ribonucleotide + NH3
-
ir
beta-nicotinamide D-ribonucleotide + H2O involvement in NAD metabolism Azotobacter vinelandii beta-nicotinate D-ribonucleotide + NH3
-
ir
nicotinamide ribonucleoside + H2O 14% activity compared to nicotinamide D-ribonucleotide Azotobacter vinelandii nicotinic acid ribonucleoside + NH3
-
ir

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
64
-
-
Azotobacter vinelandii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
64
-
preincubation without substrate: complete denaturation within 32 min Azotobacter vinelandii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.2
-
Azotobacter vinelandii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.9
-
quinolinic acid
-
Azotobacter vinelandii
4.4
-
nicotinic acid
-
Azotobacter vinelandii
7
-
beta-nicotinic acid adenine dinucleotide
-
Azotobacter vinelandii
8
-
alpha-nicotinic acid adenine dinucleotide
-
Azotobacter vinelandii
8.3
-
Isonicotinic acid
-
Azotobacter vinelandii
9.8
-
Picolinic acid
-
Azotobacter vinelandii
21
-
alpha-nicotinic acid mononucleotide
-
Azotobacter vinelandii