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Literature summary for 3.5.1.41 extracted from
- Identification and characterization of a chitin deacetylase from a metagenomic library of deep-sea sediments of the arctic ocean (2016), Gene, 590, 79-84 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| identification and characterization of a chitin deacetylase from a metagenomic library of deep-sea sediments of the arctic ocean, genotyping, phylogenetic analysis of CdaYJ, cdaYJ gene is subcloned into the pET-28a vector and recombinantly overexpressed in Escherichia coli strain BL21(DE3) | uncultured organism |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured organism | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitroacetanilide + H2O | - |
uncultured organism | 4-nitroaniline + acetate | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, recombinant enzyme, SDS-PAGE | uncultured organism |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cdaYJ | - |
uncultured organism |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
- |
uncultured organism |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 35 | over 20% of maximal activity within this range | uncultured organism |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
uncultured organism |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | over 50% of maximal activity within this range | uncultured organism |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the CdaYJ is homologous to some known chitin deacetylases and contains conserved chitin deacetylase active sites. CdaYJ protein exhibits a long N-terminal and a relative short C-terminal. Phylogenetic analysis reveals that CdaYJ shows highest homology to CDAs from Alphaproteobacteria. Construction of metagenomic library of the Arctic deep-sea sediments, phylogenetic analysis of CdaYJ | uncultured organism |
| additional information | identification and characterization of a chitin deacetylase from a metagenomic library of deep-sea sediments of the arctic ocean, genotyping | uncultured organism |
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Release 2023.1 (January 2023)
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