Cloned (Comment) | Organism |
---|---|
gene nodB, recombinant overexpression of His-tagged enzyme NodB in Escherichia coli strain BL21(DE3), recombinant expression of Trx-NodB. The recombinant enzyme expressed in a soluble and catalytically active form is then efficiently used as biocatalyst | Sinorhizobium meliloti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.379 | - |
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc | pH 7.2, 30°C, recombinant His-tagged enzyme | Sinorhizobium meliloti | |
1.53 | - |
GlcNAc-beta-(1-4)-GlcNAc | pH 7.2, 30°C, recombinant His-tagged enzyme | Sinorhizobium meliloti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | P02963 | i.e. Ensifer meliloti or Sinorhizobium meliloti | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylated chitosan polymer + H2O | increasing activity with increasing chain length | Sinorhizobium meliloti | ? | - |
? | |
GlcNAc-beta-(1-4)-GlcNAc + H2O | - |
Sinorhizobium meliloti | GlcN-beta-(1-4)-GlcN + 2 acetate | - |
? | |
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc + H2O | - |
Sinorhizobium meliloti | ? | - |
? | |
additional information | the reaction mechanism governs the regioselectivity | Sinorhizobium meliloti | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28500, recombinant His-tagged enzyme, SDS-PAGE | Sinorhizobium meliloti |
Synonyms | Comment | Organism |
---|---|---|
NodB | - |
Sinorhizobium meliloti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant enzyme | Sinorhizobium meliloti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
GlcNAc-beta-(1-4)-GlcNAc | pH 7.2, 30°C, recombinant His-tagged enzyme | Sinorhizobium meliloti | |
0.45 | - |
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc | pH 7.2, 30°C, recombinant His-tagged enzyme | Sinorhizobium meliloti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | recombinant enzyme | Sinorhizobium meliloti |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.248 | - |
GlcNAc-beta-(1-4)-GlcNAc | pH 7.2, 30°C, recombinant His-tagged enzyme | Sinorhizobium meliloti | |
1.19 | - |
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc | pH 7.2, 30°C, recombinant His-tagged enzyme | Sinorhizobium meliloti |