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Literature summary for 3.5.1.41 extracted from

  • Chambon, R.; Pradeau, S.; Fort, S.; Cottaz, S.; Armand, S.
    High yield production of Rhizobium NodB chitin deacetylase and its use for in vitro synthesis of lipo-chitinoligosaccharide precursors (2017), Carbohydr. Res., 442, 25-30 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene nodB, recombinant overexpression of His-tagged enzyme NodB in Escherichia coli strain BL21(DE3), recombinant expression of Trx-NodB. The recombinant enzyme expressed in a soluble and catalytically active form is then efficiently used as biocatalyst Sinorhizobium meliloti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.379
-
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc pH 7.2, 30°C, recombinant His-tagged enzyme Sinorhizobium meliloti
1.53
-
GlcNAc-beta-(1-4)-GlcNAc pH 7.2, 30°C, recombinant His-tagged enzyme Sinorhizobium meliloti

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti P02963 i.e. Ensifer meliloti or Sinorhizobium meliloti
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylated chitosan polymer + H2O increasing activity with increasing chain length Sinorhizobium meliloti ?
-
?
GlcNAc-beta-(1-4)-GlcNAc + H2O
-
Sinorhizobium meliloti GlcN-beta-(1-4)-GlcN + 2 acetate
-
?
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc + H2O
-
Sinorhizobium meliloti ?
-
?
additional information the reaction mechanism governs the regioselectivity Sinorhizobium meliloti ?
-
?

Subunits

Subunits Comment Organism
? x * 28500, recombinant His-tagged enzyme, SDS-PAGE Sinorhizobium meliloti

Synonyms

Synonyms Comment Organism
NodB
-
Sinorhizobium meliloti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
recombinant enzyme Sinorhizobium meliloti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
GlcNAc-beta-(1-4)-GlcNAc pH 7.2, 30°C, recombinant His-tagged enzyme Sinorhizobium meliloti
0.45
-
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc pH 7.2, 30°C, recombinant His-tagged enzyme Sinorhizobium meliloti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 recombinant enzyme Sinorhizobium meliloti

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.248
-
GlcNAc-beta-(1-4)-GlcNAc pH 7.2, 30°C, recombinant His-tagged enzyme Sinorhizobium meliloti
1.19
-
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcN-beta-(1-4)-GlcNAc pH 7.2, 30°C, recombinant His-tagged enzyme Sinorhizobium meliloti