Cloned (Comment) | Organism |
---|---|
phylogenetic tree, recombinant expression of Strep-tagged enzyme in Hansenula polymorpha strain RB11 | Podospora anserina |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of enzyme deletion mutants lacking one or both chitin-binding domains, CBDs | Podospora anserina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 80.9% inhibition at 10 mM, 19.4% at 1 mM | Podospora anserina | |
EDTA | moderate inhibition at 10 mM | Podospora anserina | |
Fe2+ | 70.5% inhibition at 10 mM | Podospora anserina | |
Mn2+ | 9% activating at 1 mM, 22.6% inhibition at 10 mM | Podospora anserina | |
additional information | no inhibition by 100 mM acetate | Podospora anserina | |
Zn2+ | 98.3% inhibition at 10 mM, 68.3% at 1 mM | Podospora anserina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 9% activating at 1 mM, 22.6% inhibition at 10 mM | Podospora anserina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Podospora anserina | B2AAQ0 | i.e. Pleurage anserina | - |
Podospora anserina ATCC MYA-4624 | B2AAQ0 | i.e. Pleurage anserina | - |
Podospora anserina DSM 980 | B2AAQ0 | i.e. Pleurage anserina | - |
Podospora anserina FGSC 10383 | B2AAQ0 | i.e. Pleurage anserina | - |
Podospora anserina S | B2AAQ0 | i.e. Pleurage anserina | - |
Purification (Comment) | Organism |
---|---|
recombinant Strep-tagged enzyme from Hansenula polymorpha strain RB11 by StrepTactin affinity chromatography | Podospora anserina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylated chitin oligomer + H2O | - |
Podospora anserina | ? | - |
? | |
acetylated chitin oligomer + H2O | - |
Podospora anserina ATCC MYA-4624 | ? | - |
? | |
acetylated chitin oligomer + H2O | - |
Podospora anserina DSM 980 | ? | - |
? | |
acetylated chitin oligomer + H2O | - |
Podospora anserina FGSC 10383 | ? | - |
? | |
acetylated chitin oligomer + H2O | - |
Podospora anserina S | ? | - |
? | |
acetylated chitosan oligomer + H2O | increasing activity with increasing degree of acetylation | Podospora anserina | ? | - |
? | |
alpha-chitin + H2O | - |
Podospora anserina | ? | - |
? | |
alpha-chitin + H2O | - |
Podospora anserina ATCC MYA-4624 | ? | - |
? | |
alpha-chitin + H2O | - |
Podospora anserina DSM 980 | ? | - |
? | |
alpha-chitin + H2O | - |
Podospora anserina FGSC 10383 | ? | - |
? | |
alpha-chitin + H2O | - |
Podospora anserina S | ? | - |
? | |
beta-chitin + H2O | - |
Podospora anserina | ? | - |
? | |
beta-chitin + H2O | - |
Podospora anserina ATCC MYA-4624 | ? | - |
? | |
beta-chitin + H2O | - |
Podospora anserina DSM 980 | ? | - |
? | |
beta-chitin + H2O | - |
Podospora anserina FGSC 10383 | ? | - |
? | |
beta-chitin + H2O | - |
Podospora anserina S | ? | - |
? | |
chitin pentamer + H2O | - |
Podospora anserina | ? | - |
? | |
colloidal chitin + H2O | high activity | Podospora anserina | ? | - |
? | |
colloidal chitin + H2O | high activity | Podospora anserina ATCC MYA-4624 | ? | - |
? | |
colloidal chitin + H2O | high activity | Podospora anserina DSM 980 | ? | - |
? | |
colloidal chitin + H2O | high activity | Podospora anserina FGSC 10383 | ? | - |
? | |
colloidal chitin + H2O | high activity | Podospora anserina S | ? | - |
? | |
additional information | analysis of the enzyme's mode of action on chitin oligomers by quantitative mass-spectrometric sequencing | Podospora anserina | ? | - |
? | |
additional information | analysis of the enzyme's mode of action on chitin oligomers by quantitative mass-spectrometric sequencing | Podospora anserina ATCC MYA-4624 | ? | - |
? | |
additional information | analysis of the enzyme's mode of action on chitin oligomers by quantitative mass-spectrometric sequencing | Podospora anserina DSM 980 | ? | - |
? | |
additional information | analysis of the enzyme's mode of action on chitin oligomers by quantitative mass-spectrometric sequencing | Podospora anserina FGSC 10383 | ? | - |
? | |
additional information | analysis of the enzyme's mode of action on chitin oligomers by quantitative mass-spectrometric sequencing | Podospora anserina S | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, about, sequence calculation, x * 35500, recombinant enzyme, SDS-PAGE | Podospora anserina |
Synonyms | Comment | Organism |
---|---|---|
CDA | - |
Podospora anserina |
PaCDA | - |
Podospora anserina |
PODANS_1_4780 | - |
Podospora anserina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
broad optimum at 40-60°C | Podospora anserina |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 90 | activity range, profile overview | Podospora anserina |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
purified recombinant enzyme, half-life is 11.8 min | Podospora anserina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
- |
Podospora anserina |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 12 | activity range, almost linear increase of activity over the pH range 4.0-10.5 | Podospora anserina |
General Information | Comment | Organism |
---|---|---|
evolution | the CDA from Podospora anserina (PaCDA) is closely related to Colletotrichum lindemuthianum CDA in the catalytic domain, but unique in having two chitin-binding domains. The catalytic domain of PaCDA is also functionally similar to Colletotrichum lindemuthianum CDA, though differing in detail | Podospora anserina |
additional information | mode of action on chitin, overview | Podospora anserina |
physiological function | proposed function of the enzyme in conversion of the insoluble substrate colloidal chitin | Podospora anserina |