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Literature summary for 3.5.1.41 extracted from

  • Naqvi, S.; Cord-Landwehr, S.; Singh, R.; Bernard, F.; Kolkenbrock, S.; El Gueddari, N.E.; Moerschbacher, B.M.
    A recombinant fungal chitin deacetylase produces fully defined chitosan oligomers with novel patterns of acetylation (2016), Appl. Environ. Microbiol., 82, 6645-6655 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.41

Application

Application Comment Organism
synthesis recombinant enzyme is used to produce partially acetylated chitosan oligosaccharides from chitin oligomers, whereby the pronounced regioselectivity of the enzyme leads to the production of defined products with novel patterns of acetylation Puccinia graminis f. sp. tritici

Cloned(Commentary)

Cloned (Comment) Organism
gene cda1, sequence comparison with ClCDA from Colletotrichum lindemuthianum, recombinant expression in Escherichia coli and secretion into the periplasmic space of the bacterial host, recombinant expression of MBP-tagged, StrepII-tagged, or His-tagged enzyme in Escherichia coli strains BL21(DE3), ER2508, or Rosetta 2(DE3), optimal expression in Escherichia coli strain Rosetta2(DE3)/pLysSRARE as MBP-tagged enzyme Puccinia graminis f. sp. tritici

Organism

Organism UniProt Comment Textmining
Puccinia graminis f. sp. tritici B0B0H7
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 activity of recombinant PgtCDA produced in different bacterial expression strains with chitin hexamer at pH 8.0 and 37°C, overview Puccinia graminis f. sp. tritici

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylated chitin polymer + H2O increasing activity with increasing chain length Puccinia graminis f. sp. tritici ?
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 ?
acetylated chitosan polymer + H2O increasing activity with increasing degree of acetylation Puccinia graminis f. sp. tritici ?
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 ?
alpha-chitin + H2O
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 Puccinia graminis f. sp. tritici ?
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 ?
beta-chitin + H2O
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 Puccinia graminis f. sp. tritici ?
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 ?
chitin hexamer + H2O
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 Puccinia graminis f. sp. tritici ?
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 ?
colloidal chitin + H2O high activity Puccinia graminis f. sp. tritici ?
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 ?
additional information mass spectrometric sequencing of the products obtained by enzymatic deacetylation of chitin oligomers, i.e. tetramers to hexamers, revealing that PgtCDA generates paCOS with specific acetylation patterns of A-A-D-D, A-A-D-D-D, and A-A-D-D-D-D, respectively (A = GlcNAc, D = GlcN), indicating that PgtCDA cannot deacetylate the two GlcNAc units closest to the oligomer's nonreducing end. Detailed product analysis, detection method, overview. Pronounced regioselectivity of the enzyme Puccinia graminis f. sp. tritici ?
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Synonyms

Synonyms Comment Organism
CDA1
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 Puccinia graminis f. sp. tritici
PgtCDA
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 Puccinia graminis f. sp. tritici

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 recombinant enzyme Puccinia graminis f. sp. tritici

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 activity range, profile overview, recombinant enzyme Puccinia graminis f. sp. tritici

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9 recombinant enzyme Puccinia graminis f. sp. tritici

pH Range

pH Minimum pH Maximum Comment Organism
5 10 activity range, profile overview, recombinant enzyme Puccinia graminis f. sp. tritici

General Information

General Information Comment Organism
additional information molecular structure-function relationships, three-dimensional modeling. Bioinformatic analysis of the chitin deacetylase PgtCDA gene Puccinia graminis f. sp. tritici