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Literature summary for 3.5.1.41 extracted from
- Novel characteristics of chitin deacetylase from Colletotrichum lindemuthianum: production of fully acetylated chitooligomers, and hydrolysis of deacetylated chitooligomers (2014), Process Biochem., 49, 1936-1940.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression in Pichia pastoris | Colletotrichum lindemuthianum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Colletotrichum lindemuthianum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| monoacetylated chitopentaose + H2O | - |
Colletotrichum lindemuthianum | chitopentaose + acetate | - |
? |  |
| monoacetylated chitotetraose + H2O | - |
Colletotrichum lindemuthianum | chitotetraose + acetate | - |
? | |
| monoacetylated chitotriose + H2O | - |
Colletotrichum lindemuthianum | chitotriose + acetate | - |
? | |
| additional information | the recombinant enzyme deacetylates the complex oligosaccharide substrates with various acetyls produced by endo-chitosanase from Aspergillus fumigatus hydrolyzing chitosan. Substrate and product identification by mass spectrometric analysis, overview. (GlcNAc)4 is fully deacetylated into (GlcN)4, and the enzyme deacetylates (GlcNAc)4 and (GlcNAc)5 much faster than (GlcNAc)3 and (GlcNAc)2. Chitotriose is also fully deacetylated through a random deacetylation process, while chitobiose (GlcNAc)2, is only deacetylated at the non-reducing GlcNAc residue. The enzyme exclusively produces Glc-NGlcNAc, and cannot deacetylate GlcNAc | Colletotrichum lindemuthianum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ClCDA | - |
Colletotrichum lindemuthianum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Colletotrichum lindemuthianum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Colletotrichum lindemuthianum |
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Release 2023.1 (January 2023)
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