Application | Comment | Organism |
---|---|---|
synthesis | the enzyme might be useful for large scale production of chitosan from chitin | Colletotrichum lindemuthianum |
Cloned (Comment) | Organism |
---|---|
expression of His6-tagged enzyme in Pichia pastoris strain GS115, secretion of the recombinant enzyme to the culture supernatant | Colletotrichum lindemuthianum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibitory at higher concentration | Colletotrichum lindemuthianum | |
Cu2+ | highly inhibitory at higher concentration | Colletotrichum lindemuthianum | |
Fe2+ | highly inhibitory at higher concentration | Colletotrichum lindemuthianum | |
Mg2+ | inhibitory at higher concentration | Colletotrichum lindemuthianum | |
Ni2+ | potent inhibition | Colletotrichum lindemuthianum | |
Zn2+ | potent inhibition | Colletotrichum lindemuthianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
hexa-N-acetyl-chitohexaose | pH 8.0, 50°C, recombinant enzyme | Colletotrichum lindemuthianum | |
5.7 | - |
penta-N-acetyl-chitopentaose | pH 8.0, 50°C, recombinant enzyme | Colletotrichum lindemuthianum | |
8.9 | - |
tetra-N-acetyl-chitotetraose | pH 8.0, 50°C, recombinant enzyme | Colletotrichum lindemuthianum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates by 26% | Colletotrichum lindemuthianum | |
additional information | Li+ and Mn2+ have no effect on enzyme activity | Colletotrichum lindemuthianum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25200 | - |
x * 25200, recombinant His6-tagged enzyme, SDS-PAGE | Colletotrichum lindemuthianum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colletotrichum lindemuthianum | Q6DWK3 | - |
- |
Colletotrichum lindemuthianum UPS9 | Q6DWK3 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | recombinant His6-tagged enzyme | Colletotrichum lindemuthianum |
proteolytic modification | the enzyme contains a signal sequence which is cleaved at Gln28 | Colletotrichum lindemuthianum |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme 364fold from Pichia pastoris strain GS115 culture supernatant by the single step process of nickel affinity chromatography | Colletotrichum lindemuthianum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity | Colletotrichum lindemuthianum |
0.2 | - |
recombinant His6-tagged enzyme in Pichia pastoris cell culture supernatant | Colletotrichum lindemuthianum |
135.6 | - |
purified recombinant His6-tagged enzyme, substrate penta-N-acetyl-chitopentaose | Colletotrichum lindemuthianum |
138.5 | - |
purified recombinant His6-tagged enzyme, substrate tetra-N-acetyl-chitotetraose | Colletotrichum lindemuthianum |
184.4 | - |
purified recombinant His6-tagged enzyme, substrate hexa-N-acetyl-chitohexaose | Colletotrichum lindemuthianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hexa-N-acetyl-chitohexaose + H2O | - |
Colletotrichum lindemuthianum | chitohexaose + acetate | - |
? | |
hexa-N-acetyl-chitohexaose + H2O | - |
Colletotrichum lindemuthianum UPS9 | chitohexaose + acetate | - |
? | |
additional information | substrate specificity, overview, no activity with chitotriose | Colletotrichum lindemuthianum | ? | - |
? | |
additional information | substrate specificity, overview, no activity with chitotriose | Colletotrichum lindemuthianum UPS9 | ? | - |
? | |
penta-N-acetyl-chitopentaose + H2O | - |
Colletotrichum lindemuthianum | chitopentaose + acetate | - |
? | |
penta-N-acetyl-chitopentaose + H2O | - |
Colletotrichum lindemuthianum UPS9 | chitopentaose + acetate | - |
? | |
tetra-N-acetyl-chitotetraose + H2O | - |
Colletotrichum lindemuthianum | chitotetraose + acetate | - |
? | |
tetra-N-acetyl-chitotetraose + H2O | - |
Colletotrichum lindemuthianum UPS9 | chitotetraose + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25200, recombinant His6-tagged enzyme, SDS-PAGE | Colletotrichum lindemuthianum |
More | comparison of enzyme amino acid sequences of strain UPS9 with those of strain ATCC 56676, overview | Colletotrichum lindemuthianum |
Synonyms | Comment | Organism |
---|---|---|
CDA | - |
Colletotrichum lindemuthianum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
with substrate tetra-N-acetyl-chitotetraose | Colletotrichum lindemuthianum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 75 | half-maximal activity at 40°C and 75°C, 30% of maximal activity at 80°C | Colletotrichum lindemuthianum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life: 1 h | Colletotrichum lindemuthianum |
90 | - |
half-life: 30 min | Colletotrichum lindemuthianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
with substrate tetra-N-acetyl-chitotetraose | Colletotrichum lindemuthianum |